Telomere Lengths and Telomerase Activity in Dog Tissues: A Potential Model System to Study Human Telomere and Telomerase Biology
Studies on telomere and telomerase biology are fundamental to the understanding of aging and age-related diseases such as cancer. However, human studies have been hindered by differences in telomere biology between humans and the classical murine animal model system. In this paper, we describe basi...
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2001-01-01
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doaj-69835f14e7234cd885467eb1fc6328482020-11-24T22:26:54ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022001-01-013435135910.1038/sj.neo.7900173Telomere Lengths and Telomerase Activity in Dog Tissues: A Potential Model System to Study Human Telomere and Telomerase BiologyLubna Nasir0Pauline Devlin1Tom Mckevitt2Gerard Rutteman3David J. Argyle4Department of Veterinary Clinical Studies, University of Glasgow Veterinary School, Bearsden Road, Glasgow G61 10H, UKWattham Centre for Pet Nutrition, Waltham on the Wolds, Melton Mobray, Leicestershire, UKDepartment of Veterinary Clinical Studies, University of Glasgow Veterinary School, Bearsden Road, Glasgow G61 10H, UKDepartment of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, PO Box 80.154, Utrecht 3508 TD, The NetherlandsDepartment of Veterinary Clinical Studies, University of Glasgow Veterinary School, Bearsden Road, Glasgow G61 10H, UK Studies on telomere and telomerase biology are fundamental to the understanding of aging and age-related diseases such as cancer. However, human studies have been hindered by differences in telomere biology between humans and the classical murine animal model system. In this paper, we describe basic studies of telomere length and telomerase activity in canine normal and neoplastic tissues and propose the dog as an alternative model system. Briefly, telomere lengths were measured in normal canine peripheral blood mononuclear cells (PBMCs), a range of normal canine tissues, and in a panel of naturally occurring soft tissue tumours by terminal restriction fragment (TRF) analysis. Further, telomerase activity was measured in canine cell lines and multiple canine tissues using a combined polymerase chain reaction/enzyme-linked immunosorbent assay method. TRF analysis in canine PBMCs and tissues demonstrated mean TRF lengths to range between 12 and 23 kbp with heterogeneity in telomere lengths being observed in a range of normal somatic tissues. In soft tissue sarcomas, two subgroups were identified with mean TRFs of 22.2 and 18.2 kbp. Telomerase activity in canine tissue was present in tumour tissue and testis with little or no activity in normal somatic tissues. These results suggest that the dog telomere biology is similar to that in humans and may represent an alternative model system for studying telomere biology and telomerase-targeted anticancer therapies. http://www.sciencedirect.com/science/article/pii/S1476558601800385telomeretelomerasedogcancermodel |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lubna Nasir Pauline Devlin Tom Mckevitt Gerard Rutteman David J. Argyle |
spellingShingle |
Lubna Nasir Pauline Devlin Tom Mckevitt Gerard Rutteman David J. Argyle Telomere Lengths and Telomerase Activity in Dog Tissues: A Potential Model System to Study Human Telomere and Telomerase Biology Neoplasia: An International Journal for Oncology Research telomere telomerase dog cancer model |
author_facet |
Lubna Nasir Pauline Devlin Tom Mckevitt Gerard Rutteman David J. Argyle |
author_sort |
Lubna Nasir |
title |
Telomere Lengths and Telomerase Activity in Dog Tissues: A Potential Model System to Study Human Telomere and Telomerase Biology |
title_short |
Telomere Lengths and Telomerase Activity in Dog Tissues: A Potential Model System to Study Human Telomere and Telomerase Biology |
title_full |
Telomere Lengths and Telomerase Activity in Dog Tissues: A Potential Model System to Study Human Telomere and Telomerase Biology |
title_fullStr |
Telomere Lengths and Telomerase Activity in Dog Tissues: A Potential Model System to Study Human Telomere and Telomerase Biology |
title_full_unstemmed |
Telomere Lengths and Telomerase Activity in Dog Tissues: A Potential Model System to Study Human Telomere and Telomerase Biology |
title_sort |
telomere lengths and telomerase activity in dog tissues: a potential model system to study human telomere and telomerase biology |
publisher |
Elsevier |
series |
Neoplasia: An International Journal for Oncology Research |
issn |
1476-5586 1522-8002 |
publishDate |
2001-01-01 |
description |
Studies on telomere and telomerase biology are fundamental to the understanding of aging and age-related diseases such as cancer. However, human studies have been hindered by differences in telomere biology between humans and the classical murine animal model system. In this paper, we describe basic studies of telomere length and telomerase activity in canine normal and neoplastic tissues and propose the dog as an alternative model system. Briefly, telomere lengths were measured in normal canine peripheral blood mononuclear cells (PBMCs), a range of normal canine tissues, and in a panel of naturally occurring soft tissue tumours by terminal restriction fragment (TRF) analysis. Further, telomerase activity was measured in canine cell lines and multiple canine tissues using a combined polymerase chain reaction/enzyme-linked immunosorbent assay method. TRF analysis in canine PBMCs and tissues demonstrated mean TRF lengths to range between 12 and 23 kbp with heterogeneity in telomere lengths being observed in a range of normal somatic tissues. In soft tissue sarcomas, two subgroups were identified with mean TRFs of 22.2 and 18.2 kbp. Telomerase activity in canine tissue was present in tumour tissue and testis with little or no activity in normal somatic tissues. These results suggest that the dog telomere biology is similar to that in humans and may represent an alternative model system for studying telomere biology and telomerase-targeted anticancer therapies.
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topic |
telomere telomerase dog cancer model |
url |
http://www.sciencedirect.com/science/article/pii/S1476558601800385 |
work_keys_str_mv |
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