Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death

Abstract Cell death, autophagy and endosomal sorting contribute to many physiological, developmental and immunological processes in plants. They are mechanistically interconnected and interdependent, but the molecular basis of their mutual regulation has only begun to emerge in plants. Here, we desc...

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Main Authors:	Arpaporn Sutipatanasomboon, Stefanie Herberth, Ellen G. Alwood, Heidrun Häweker, Britta Müller, Mojgan Shahriari, Anke Y. Zienert, Birger Marin, Silke Robatzek, Gerrit J. K. Praefcke, Kathryn R. Ayscough, Martin Hülskamp, Swen Schellmann
Format:	Article
Language:	English
Published:	Nature Publishing Group 2017-08-01
Series:	Scientific Reports
Online Access:	https://doi.org/10.1038/s41598-017-08577-8

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spelling	doaj-6992c0ebf240486fa7c47bdd6f4fefa62020-12-08T02:45:06ZengNature Publishing GroupScientific Reports2045-23222017-08-017111410.1038/s41598-017-08577-8Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell deathArpaporn Sutipatanasomboon0Stefanie Herberth1Ellen G. Alwood2Heidrun Häweker3Britta Müller4Mojgan Shahriari5Anke Y. Zienert6Birger Marin7Silke Robatzek8Gerrit J. K. Praefcke9Kathryn R. Ayscough10Martin Hülskamp11Swen Schellmann12Botanik III, Biocenter, Universtiy of CologneBotanik III, Biocenter, Universtiy of CologneDepartment of Biomedical Science, The University of SheffieldThe Sainsbury Laboratory, Norwich Research ParkBotanik III, Biocenter, Universtiy of CologneBotanik III, Biocenter, Universtiy of CologneInstitut für Genetik, Universtiy of CologneBotanik I, Biocenter, Universtiy of CologneThe Sainsbury Laboratory, Norwich Research ParkInstitut für Genetik, Universtiy of CologneDepartment of Biomedical Science, The University of SheffieldBotanik III, Biocenter, Universtiy of CologneBotanik III, Biocenter, Universtiy of CologneAbstract Cell death, autophagy and endosomal sorting contribute to many physiological, developmental and immunological processes in plants. They are mechanistically interconnected and interdependent, but the molecular basis of their mutual regulation has only begun to emerge in plants. Here, we describe the identification and molecular characterization of CELL DEATH RELATED ENDOSOMAL FYVE/SYLF PROTEIN 1 (CFS1). The CFS1 protein interacts with the ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT I (ESCRT-I) component ELCH (ELC) and is localized at ESCRT-I-positive late endosomes likely through its PI3P and actin binding SH3YL1 Ysc84/Lsb4p Lsb3p plant FYVE (SYLF) domain. Mutant alleles of cfs1 exhibit auto-immune phenotypes including spontaneous lesions that show characteristics of hypersensitive response (HR). Autoimmunity in cfs1 is dependent on ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)-mediated effector-triggered immunity (ETI) but independent from salicylic acid. Additionally, cfs1 mutants accumulate the autophagy markers ATG8 and NBR1 independently from EDS1. We hypothesize that CFS1 acts at the intersection of autophagosomes and endosomes and contributes to cellular homeostasis by mediating autophagosome turnover.https://doi.org/10.1038/s41598-017-08577-8
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Arpaporn Sutipatanasomboon Stefanie Herberth Ellen G. Alwood Heidrun Häweker Britta Müller Mojgan Shahriari Anke Y. Zienert Birger Marin Silke Robatzek Gerrit J. K. Praefcke Kathryn R. Ayscough Martin Hülskamp Swen Schellmann
spellingShingle	Arpaporn Sutipatanasomboon Stefanie Herberth Ellen G. Alwood Heidrun Häweker Britta Müller Mojgan Shahriari Anke Y. Zienert Birger Marin Silke Robatzek Gerrit J. K. Praefcke Kathryn R. Ayscough Martin Hülskamp Swen Schellmann Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death Scientific Reports
author_facet	Arpaporn Sutipatanasomboon Stefanie Herberth Ellen G. Alwood Heidrun Häweker Britta Müller Mojgan Shahriari Anke Y. Zienert Birger Marin Silke Robatzek Gerrit J. K. Praefcke Kathryn R. Ayscough Martin Hülskamp Swen Schellmann
author_sort	Arpaporn Sutipatanasomboon
title	Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death
title_short	Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death
title_full	Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death
title_fullStr	Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death
title_full_unstemmed	Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death
title_sort	disruption of the plant-specific cfs1 gene impairs autophagosome turnover and triggers eds1-dependent cell death
publisher	Nature Publishing Group
series	Scientific Reports
issn	2045-2322
publishDate	2017-08-01
description	Abstract Cell death, autophagy and endosomal sorting contribute to many physiological, developmental and immunological processes in plants. They are mechanistically interconnected and interdependent, but the molecular basis of their mutual regulation has only begun to emerge in plants. Here, we describe the identification and molecular characterization of CELL DEATH RELATED ENDOSOMAL FYVE/SYLF PROTEIN 1 (CFS1). The CFS1 protein interacts with the ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT I (ESCRT-I) component ELCH (ELC) and is localized at ESCRT-I-positive late endosomes likely through its PI3P and actin binding SH3YL1 Ysc84/Lsb4p Lsb3p plant FYVE (SYLF) domain. Mutant alleles of cfs1 exhibit auto-immune phenotypes including spontaneous lesions that show characteristics of hypersensitive response (HR). Autoimmunity in cfs1 is dependent on ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)-mediated effector-triggered immunity (ETI) but independent from salicylic acid. Additionally, cfs1 mutants accumulate the autophagy markers ATG8 and NBR1 independently from EDS1. We hypothesize that CFS1 acts at the intersection of autophagosomes and endosomes and contributes to cellular homeostasis by mediating autophagosome turnover.
url	https://doi.org/10.1038/s41598-017-08577-8
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Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death

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