Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71
Subunit vaccines consist of non-genetic material, such as peptides or proteins. They are considered safe because they have fewer side effects; however, they have low immunogenicity when used alone. We aimed to enhance the immune response of peptide-based vaccines by using self-assembled multimeric p...
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doaj-6998df84da234913ae17ca6240f61cbf2020-11-27T08:04:47ZengMDPI AGNanomaterials2079-49912020-11-01102342234210.3390/nano10122342Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71Yu-Gyeong Kim0Yunsu Lee1Joo Hee Kim2Sun-Young Chang3Jong-Wha Jung4Woo-Jae Chung5Hyo-Eon Jin6College of Pharmacy, Ajou University, Suwon 16499, KoreaCollege of Pharmacy, Ajou University, Suwon 16499, KoreaCollege of Pharmacy, Ajou University, Suwon 16499, KoreaCollege of Pharmacy, Ajou University, Suwon 16499, KoreaResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Kyungpook National University, Daegu 41566, KoreaDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, KoreaCollege of Pharmacy, Ajou University, Suwon 16499, KoreaSubunit vaccines consist of non-genetic material, such as peptides or proteins. They are considered safe because they have fewer side effects; however, they have low immunogenicity when used alone. We aimed to enhance the immune response of peptide-based vaccines by using self-assembled multimeric peptide amphiphiles (PAs). We designed two epitope PAs by conjugating epitope peptides from Enterovirus 71 (EV71) virus particle (VP) 1 and VP3 capsid proteins with different fatty acid chain lengths (VP1PA and VP3PA). These PAs self-assembled into supramolecular structures at a physiological pH, and the resulting structures were characterized using atomic force microscopy. Multi-epitope PAs (m-PAs) consisted of a 1:1 mixture of VP1PA and VP3PA solutions. To evaluate immunogenicity, m-PA constructs were injected with adjuvant subcutaneously into female Balb/c mice. Levels of antigen-specific immunoglobulin G (IgG) and IgG1 in m-PA-injected mice serum samples were analyzed using ELISA and Western blotting. Additionally, cytokine production stimulated by each antigen was measured in splenocytes cultured from immunized mice groups. We found that m-PA showed improved humoral and cellular immune responses compared to the control and peptide groups. The sera from m-PA immunized mice group could neutralize EV71 infection and protect host cells. Thus, self-assembled m-PAs can promote a protective immune response and can be developed as a potential platform technology to produce peptide vaccines against infectious viral diseases.https://www.mdpi.com/2079-4991/10/12/2342peptide amphiphileimmune stimulatorvaccineviral infectious diseaseEnterovirus 71 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu-Gyeong Kim Yunsu Lee Joo Hee Kim Sun-Young Chang Jong-Wha Jung Woo-Jae Chung Hyo-Eon Jin |
spellingShingle |
Yu-Gyeong Kim Yunsu Lee Joo Hee Kim Sun-Young Chang Jong-Wha Jung Woo-Jae Chung Hyo-Eon Jin Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71 Nanomaterials peptide amphiphile immune stimulator vaccine viral infectious disease Enterovirus 71 |
author_facet |
Yu-Gyeong Kim Yunsu Lee Joo Hee Kim Sun-Young Chang Jong-Wha Jung Woo-Jae Chung Hyo-Eon Jin |
author_sort |
Yu-Gyeong Kim |
title |
Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71 |
title_short |
Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71 |
title_full |
Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71 |
title_fullStr |
Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71 |
title_full_unstemmed |
Self-Assembled Multi-Epitope Peptide Amphiphiles Enhance the Immune Response against Enterovirus 71 |
title_sort |
self-assembled multi-epitope peptide amphiphiles enhance the immune response against enterovirus 71 |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2020-11-01 |
description |
Subunit vaccines consist of non-genetic material, such as peptides or proteins. They are considered safe because they have fewer side effects; however, they have low immunogenicity when used alone. We aimed to enhance the immune response of peptide-based vaccines by using self-assembled multimeric peptide amphiphiles (PAs). We designed two epitope PAs by conjugating epitope peptides from Enterovirus 71 (EV71) virus particle (VP) 1 and VP3 capsid proteins with different fatty acid chain lengths (VP1PA and VP3PA). These PAs self-assembled into supramolecular structures at a physiological pH, and the resulting structures were characterized using atomic force microscopy. Multi-epitope PAs (m-PAs) consisted of a 1:1 mixture of VP1PA and VP3PA solutions. To evaluate immunogenicity, m-PA constructs were injected with adjuvant subcutaneously into female Balb/c mice. Levels of antigen-specific immunoglobulin G (IgG) and IgG1 in m-PA-injected mice serum samples were analyzed using ELISA and Western blotting. Additionally, cytokine production stimulated by each antigen was measured in splenocytes cultured from immunized mice groups. We found that m-PA showed improved humoral and cellular immune responses compared to the control and peptide groups. The sera from m-PA immunized mice group could neutralize EV71 infection and protect host cells. Thus, self-assembled m-PAs can promote a protective immune response and can be developed as a potential platform technology to produce peptide vaccines against infectious viral diseases. |
topic |
peptide amphiphile immune stimulator vaccine viral infectious disease Enterovirus 71 |
url |
https://www.mdpi.com/2079-4991/10/12/2342 |
work_keys_str_mv |
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