A Panel of Plasma Exosomal miRNAs as Potential Biomarkers for Differential Diagnosis of Thyroid Nodules
Background: A liquid biopsy using circulating exosomal genetic materials provides new insights for thyroid cancer diagnosis. This study aimed to identify plasma-derived exosomal biomarkers that could be used for early detection of papillary thyroid carcinoma (PTC).Method: Exosomal miRNAs in plasma w...
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doaj-69a4328f69a14145b5cb6ae95ed5a5582020-11-25T02:37:13ZengFrontiers Media S.A.Frontiers in Genetics1664-80212020-05-011110.3389/fgene.2020.00449539614A Panel of Plasma Exosomal miRNAs as Potential Biomarkers for Differential Diagnosis of Thyroid NodulesMeihua Liang0Siming Yu1Shuli Tang2Lu Bai3Jianan Cheng4Yuanlong Gu5Shuang Li6Xin Zheng7Lian Duan8Liang Wang9Yanqiao Zhang10Xiaoyi Huang11Department of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Pharmacy, Drug Clinical Trails Institution, Peking University Shenzhen Hospital, Shenzhen, ChinaDepartment of Gastrointestinal Medical Oncology, Harbin Medical University Cancer Hospital, Harbin, ChinaBiotherapy Center, Harbin Medical University Cancer Hospital, Harbin, ChinaDepartment of Gastrointestinal Medical Oncology, Harbin Medical University Cancer Hospital, Harbin, ChinaHematology Oncology, Taizhou Municipal Hospital, Taizhou, ChinaDepartment of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin, ChinaCollege of Bioinformatics Science and Technology, Harbin Medical University, Harbin, ChinaCollege of Bioinformatics Science and Technology, Harbin Medical University, Harbin, ChinaMoffitt Cancer Center, Tampa, FL, United StatesDepartment of Gastrointestinal Medical Oncology, Harbin Medical University Cancer Hospital, Harbin, ChinaBiotherapy Center, Harbin Medical University Cancer Hospital, Harbin, ChinaBackground: A liquid biopsy using circulating exosomal genetic materials provides new insights for thyroid cancer diagnosis. This study aimed to identify plasma-derived exosomal biomarkers that could be used for early detection of papillary thyroid carcinoma (PTC).Method: Exosomal miRNAs in plasma were isolated from patients with benign thyroid nodules and patients with PTC. Profiling of exosomal miRNA was performed using RNA sequencing (RNA-seq) to identify miRNA candidates and differentiate the benign from malignant. The validation cohort consisted of 30 patients with benign thyroid nodules, 35 PTC patients, and 31 healthy individuals. Real-time PCR was used to quantify the expression of miRNA candidates. The diagnostic potential of the candidates was evaluated by receiver operating characteristic (ROC) curves.Results: After RNA-seq, eight plasma exosomal miRNAs were selected as candidates. Further validation indicated that the levels of exosomal miR-16-2-3p, miR-223-5p, miR-34c-5p, miR-182-5p, miR-223-3p, and miR-146b-5p were significantly lower in nodules compared to healthy controls (p < 0.0001), whereas miR-16-2-3p and miR-223-5p were significantly higher in the PTC cases than in those with benign nodules (p < 0.05). ROC analyses revealed that the above six miRNAs were potent indicators for detection of thyroid nodules. Meanwhile, miR-16-2-3p and miR-223-5p can be utilized for detecting PTC from benign nodules. Additionally, combined miRNA panels showed increased diagnostic sensitivities and specificities compared to single miRNA markers.Conclusion: Six aberrantly expressed plasma exosomal miRNAs may be used as diagnostic biomarkers to differentiate thyroid nodules from healthy individuals. The panel consisting of miR-16-2-3p, miR-223-5p, miR-101-3p, and miR-34c-5p are eligible for discriminating benign from malignant thyroid nodules.https://www.frontiersin.org/article/10.3389/fgene.2020.00449/fullthyroid cancerthyroid noduleexosomemiRNAliquid biopsycancer diagnosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Meihua Liang Siming Yu Shuli Tang Lu Bai Jianan Cheng Yuanlong Gu Shuang Li Xin Zheng Lian Duan Liang Wang Yanqiao Zhang Xiaoyi Huang |
spellingShingle |
Meihua Liang Siming Yu Shuli Tang Lu Bai Jianan Cheng Yuanlong Gu Shuang Li Xin Zheng Lian Duan Liang Wang Yanqiao Zhang Xiaoyi Huang A Panel of Plasma Exosomal miRNAs as Potential Biomarkers for Differential Diagnosis of Thyroid Nodules Frontiers in Genetics thyroid cancer thyroid nodule exosome miRNA liquid biopsy cancer diagnosis |
author_facet |
Meihua Liang Siming Yu Shuli Tang Lu Bai Jianan Cheng Yuanlong Gu Shuang Li Xin Zheng Lian Duan Liang Wang Yanqiao Zhang Xiaoyi Huang |
author_sort |
Meihua Liang |
title |
A Panel of Plasma Exosomal miRNAs as Potential Biomarkers for Differential Diagnosis of Thyroid Nodules |
title_short |
A Panel of Plasma Exosomal miRNAs as Potential Biomarkers for Differential Diagnosis of Thyroid Nodules |
title_full |
A Panel of Plasma Exosomal miRNAs as Potential Biomarkers for Differential Diagnosis of Thyroid Nodules |
title_fullStr |
A Panel of Plasma Exosomal miRNAs as Potential Biomarkers for Differential Diagnosis of Thyroid Nodules |
title_full_unstemmed |
A Panel of Plasma Exosomal miRNAs as Potential Biomarkers for Differential Diagnosis of Thyroid Nodules |
title_sort |
panel of plasma exosomal mirnas as potential biomarkers for differential diagnosis of thyroid nodules |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2020-05-01 |
description |
Background: A liquid biopsy using circulating exosomal genetic materials provides new insights for thyroid cancer diagnosis. This study aimed to identify plasma-derived exosomal biomarkers that could be used for early detection of papillary thyroid carcinoma (PTC).Method: Exosomal miRNAs in plasma were isolated from patients with benign thyroid nodules and patients with PTC. Profiling of exosomal miRNA was performed using RNA sequencing (RNA-seq) to identify miRNA candidates and differentiate the benign from malignant. The validation cohort consisted of 30 patients with benign thyroid nodules, 35 PTC patients, and 31 healthy individuals. Real-time PCR was used to quantify the expression of miRNA candidates. The diagnostic potential of the candidates was evaluated by receiver operating characteristic (ROC) curves.Results: After RNA-seq, eight plasma exosomal miRNAs were selected as candidates. Further validation indicated that the levels of exosomal miR-16-2-3p, miR-223-5p, miR-34c-5p, miR-182-5p, miR-223-3p, and miR-146b-5p were significantly lower in nodules compared to healthy controls (p < 0.0001), whereas miR-16-2-3p and miR-223-5p were significantly higher in the PTC cases than in those with benign nodules (p < 0.05). ROC analyses revealed that the above six miRNAs were potent indicators for detection of thyroid nodules. Meanwhile, miR-16-2-3p and miR-223-5p can be utilized for detecting PTC from benign nodules. Additionally, combined miRNA panels showed increased diagnostic sensitivities and specificities compared to single miRNA markers.Conclusion: Six aberrantly expressed plasma exosomal miRNAs may be used as diagnostic biomarkers to differentiate thyroid nodules from healthy individuals. The panel consisting of miR-16-2-3p, miR-223-5p, miR-101-3p, and miR-34c-5p are eligible for discriminating benign from malignant thyroid nodules. |
topic |
thyroid cancer thyroid nodule exosome miRNA liquid biopsy cancer diagnosis |
url |
https://www.frontiersin.org/article/10.3389/fgene.2020.00449/full |
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