Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima
Abstract Antennal and abdominal transcriptomes of males and females of the coconut hispine beetle Brontispa longissima were sequenced to identify and compare the expression patterns of genes involved in odorant reception and detection. Representative proteins from the chemosensory gene families like...
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doaj-69b6ac78ace949e781843d9497779b582020-12-08T01:05:07ZengNature Publishing GroupScientific Reports2045-23222017-06-017111310.1038/s41598-017-03263-1Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissimaShu-Ying Bin0Meng-Qiu Qu1Ke-Ming Li2Zheng-Qiang Peng3Zhong-Zhen Wu4Jin-Tian Lin5Institute for Management of Invasive Alien Species, 314 Yingdong teaching building, Zhongkai University of Agriculture and EngineeringInstitute for Management of Invasive Alien Species, 314 Yingdong teaching building, Zhongkai University of Agriculture and EngineeringInstitute of Banana and Plantain, Chinese Academy of Tropical Agricultural SciencesInstitute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural SciencesInstitute for Management of Invasive Alien Species, 314 Yingdong teaching building, Zhongkai University of Agriculture and EngineeringInstitute for Management of Invasive Alien Species, 314 Yingdong teaching building, Zhongkai University of Agriculture and EngineeringAbstract Antennal and abdominal transcriptomes of males and females of the coconut hispine beetle Brontispa longissima were sequenced to identify and compare the expression patterns of genes involved in odorant reception and detection. Representative proteins from the chemosensory gene families likely essential for insect olfaction were identified. These include 48 odorant receptors (ORs), 19 ionotropic receptors (IRs), 4 sensory neuron membrane proteins (SNMPs), 34 odorant binding proteins (OBPs) and 16 chemosensory proteins (CSPs). Phylogenetic analysis revealed the evolutionary relationship of these proteins with homologs from Coleopterans or other insects, and led to the identification of putative aggregation pheromone receptors in B. longissima. Comparative expression analysis performed by calculating FPKM values were also validated using quantitative real time-PCR (qPCR). The results revealed that all ORs and antennal IRs, two IR co-receptors (BlonIR8a and BlonIR25a) and one SNMP (BlonSNMP1a) were predominantly expressed in antennae when compared to abdomens, and approximately half of the OBPs (19) and CSPs (7) were enriched in antennae. These findings for the first time reveal the identification of key molecular components in B. longissima olfaction and provide a valuable resource for future functional analyses of olfaction, and identification of potential targets to control this quarantine pest.https://doi.org/10.1038/s41598-017-03263-1 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shu-Ying Bin Meng-Qiu Qu Ke-Ming Li Zheng-Qiang Peng Zhong-Zhen Wu Jin-Tian Lin |
spellingShingle |
Shu-Ying Bin Meng-Qiu Qu Ke-Ming Li Zheng-Qiang Peng Zhong-Zhen Wu Jin-Tian Lin Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima Scientific Reports |
author_facet |
Shu-Ying Bin Meng-Qiu Qu Ke-Ming Li Zheng-Qiang Peng Zhong-Zhen Wu Jin-Tian Lin |
author_sort |
Shu-Ying Bin |
title |
Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima |
title_short |
Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima |
title_full |
Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima |
title_fullStr |
Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima |
title_full_unstemmed |
Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima |
title_sort |
antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, brontispa longissima |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-06-01 |
description |
Abstract Antennal and abdominal transcriptomes of males and females of the coconut hispine beetle Brontispa longissima were sequenced to identify and compare the expression patterns of genes involved in odorant reception and detection. Representative proteins from the chemosensory gene families likely essential for insect olfaction were identified. These include 48 odorant receptors (ORs), 19 ionotropic receptors (IRs), 4 sensory neuron membrane proteins (SNMPs), 34 odorant binding proteins (OBPs) and 16 chemosensory proteins (CSPs). Phylogenetic analysis revealed the evolutionary relationship of these proteins with homologs from Coleopterans or other insects, and led to the identification of putative aggregation pheromone receptors in B. longissima. Comparative expression analysis performed by calculating FPKM values were also validated using quantitative real time-PCR (qPCR). The results revealed that all ORs and antennal IRs, two IR co-receptors (BlonIR8a and BlonIR25a) and one SNMP (BlonSNMP1a) were predominantly expressed in antennae when compared to abdomens, and approximately half of the OBPs (19) and CSPs (7) were enriched in antennae. These findings for the first time reveal the identification of key molecular components in B. longissima olfaction and provide a valuable resource for future functional analyses of olfaction, and identification of potential targets to control this quarantine pest. |
url |
https://doi.org/10.1038/s41598-017-03263-1 |
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