High-density lipoprotein (HDL) particle size and concentration changes in septic shock patients
Abstract Background Sepsis is associated with systemic inflammation that may impact lipoprotein function. In particular, high-density lipoproteins (HDLs) that display pleiotropic protective roles may be dysfunctional in septic conditions. The aim of this study was to evaluate the HDL profile and the...
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
SpringerOpen
2019-06-01
|
Series: | Annals of Intensive Care |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13613-019-0541-8 |
id |
doaj-69d2a34325f9497cb01a335bfa12c496 |
---|---|
record_format |
Article |
spelling |
doaj-69d2a34325f9497cb01a335bfa12c4962020-11-25T02:16:49ZengSpringerOpenAnnals of Intensive Care2110-58202019-06-01911910.1186/s13613-019-0541-8High-density lipoprotein (HDL) particle size and concentration changes in septic shock patientsSébastien Tanaka0Dévy Diallo1Sandrine Delbosc2Claire Genève3Nathalie Zappella4Jennyfer Yong-Sang5Jessica Patche6Anatole Harrois7Sophie Hamada8Erick Denamur9Philippe Montravers10Jacques Duranteau11Olivier Meilhac12INSERM, UMR 1188 Diabète athérothombose Réunion Océan Indien (DéTROI), Université de La RéunionInserm U1148, Laboratory for Vascular, Translational Science Bichat HospitalInserm U1148, Laboratory for Vascular, Translational Science Bichat HospitalAP-HP, Service d’Anesthésie-Réanimation, CHU Bichat-Claude BernardAP-HP, Service d’Anesthésie-Réanimation, CHU Bichat-Claude BernardINSERM, UMR 1188 Diabète athérothombose Réunion Océan Indien (DéTROI), Université de La RéunionINSERM, UMR 1188 Diabète athérothombose Réunion Océan Indien (DéTROI), Université de La RéunionAP-HP, Service d’Anesthésie-Réanimation, Hôpitaux Universitaires Paris-Sud, Université Paris-Sud, Hôpital de BicêtreAP-HP, Service d’Anesthésie-Réanimation, Hôpitaux Universitaires Paris-Sud, Université Paris-Sud, Hôpital de BicêtreUMR1137 IAME, Inserm, Laboratoire de Génétique Moléculaire, Université Paris Diderot and AP-HP, Hôpital BichatAP-HP, Service d’Anesthésie-Réanimation, CHU Bichat-Claude BernardAP-HP, Service d’Anesthésie-Réanimation, Hôpitaux Universitaires Paris-Sud, Université Paris-Sud, Hôpital de BicêtreINSERM, UMR 1188 Diabète athérothombose Réunion Océan Indien (DéTROI), Université de La RéunionAbstract Background Sepsis is associated with systemic inflammation that may impact lipoprotein function. In particular, high-density lipoproteins (HDLs) that display pleiotropic protective roles may be dysfunctional in septic conditions. The aim of this study was to evaluate the HDL profile and the inflammatory context in septic shock patients admitted to our intensive care unit (ICU). Methods In this study, 20 septic shock patients and 20 controls (ICU patients without septic shock) were included. Plasma samples were collected on days 1, 2 and 7. Total cholesterol and lipoprotein concentrations were determined. HDL profiles were obtained using the Lipoprint® System (non-denaturing electrophoresis). Quantification of pro-inflammatory cytokines (interleukin 1b, 6 and 8), cell-free DNA and lipopolysaccharide-binding protein was also performed. Results HDL concentration was statistically lower in septic shock patients than in controls. At days 1 and 2, septic patients had significantly more large-sized HDL than control patients. Patients recovered a normal lipid profile at day 7. Conclusions Our results emphasize that HDL levels are dramatically decreased in the acute phase of septic shock and that there is a shift toward large HDL particles, which may reflect a major dysfunction of these lipoproteins. Further mechanistic studies are required to explore this shift observed during sepsis.http://link.springer.com/article/10.1186/s13613-019-0541-8InflammationIntensive care unitLipoprintLipid profileSepsisHigh-density lipoproteins |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sébastien Tanaka Dévy Diallo Sandrine Delbosc Claire Genève Nathalie Zappella Jennyfer Yong-Sang Jessica Patche Anatole Harrois Sophie Hamada Erick Denamur Philippe Montravers Jacques Duranteau Olivier Meilhac |
spellingShingle |
Sébastien Tanaka Dévy Diallo Sandrine Delbosc Claire Genève Nathalie Zappella Jennyfer Yong-Sang Jessica Patche Anatole Harrois Sophie Hamada Erick Denamur Philippe Montravers Jacques Duranteau Olivier Meilhac High-density lipoprotein (HDL) particle size and concentration changes in septic shock patients Annals of Intensive Care Inflammation Intensive care unit Lipoprint Lipid profile Sepsis High-density lipoproteins |
author_facet |
Sébastien Tanaka Dévy Diallo Sandrine Delbosc Claire Genève Nathalie Zappella Jennyfer Yong-Sang Jessica Patche Anatole Harrois Sophie Hamada Erick Denamur Philippe Montravers Jacques Duranteau Olivier Meilhac |
author_sort |
Sébastien Tanaka |
title |
High-density lipoprotein (HDL) particle size and concentration changes in septic shock patients |
title_short |
High-density lipoprotein (HDL) particle size and concentration changes in septic shock patients |
title_full |
High-density lipoprotein (HDL) particle size and concentration changes in septic shock patients |
title_fullStr |
High-density lipoprotein (HDL) particle size and concentration changes in septic shock patients |
title_full_unstemmed |
High-density lipoprotein (HDL) particle size and concentration changes in septic shock patients |
title_sort |
high-density lipoprotein (hdl) particle size and concentration changes in septic shock patients |
publisher |
SpringerOpen |
series |
Annals of Intensive Care |
issn |
2110-5820 |
publishDate |
2019-06-01 |
description |
Abstract Background Sepsis is associated with systemic inflammation that may impact lipoprotein function. In particular, high-density lipoproteins (HDLs) that display pleiotropic protective roles may be dysfunctional in septic conditions. The aim of this study was to evaluate the HDL profile and the inflammatory context in septic shock patients admitted to our intensive care unit (ICU). Methods In this study, 20 septic shock patients and 20 controls (ICU patients without septic shock) were included. Plasma samples were collected on days 1, 2 and 7. Total cholesterol and lipoprotein concentrations were determined. HDL profiles were obtained using the Lipoprint® System (non-denaturing electrophoresis). Quantification of pro-inflammatory cytokines (interleukin 1b, 6 and 8), cell-free DNA and lipopolysaccharide-binding protein was also performed. Results HDL concentration was statistically lower in septic shock patients than in controls. At days 1 and 2, septic patients had significantly more large-sized HDL than control patients. Patients recovered a normal lipid profile at day 7. Conclusions Our results emphasize that HDL levels are dramatically decreased in the acute phase of septic shock and that there is a shift toward large HDL particles, which may reflect a major dysfunction of these lipoproteins. Further mechanistic studies are required to explore this shift observed during sepsis. |
topic |
Inflammation Intensive care unit Lipoprint Lipid profile Sepsis High-density lipoproteins |
url |
http://link.springer.com/article/10.1186/s13613-019-0541-8 |
work_keys_str_mv |
AT sebastientanaka highdensitylipoproteinhdlparticlesizeandconcentrationchangesinsepticshockpatients AT devydiallo highdensitylipoproteinhdlparticlesizeandconcentrationchangesinsepticshockpatients AT sandrinedelbosc highdensitylipoproteinhdlparticlesizeandconcentrationchangesinsepticshockpatients AT clairegeneve highdensitylipoproteinhdlparticlesizeandconcentrationchangesinsepticshockpatients AT nathaliezappella highdensitylipoproteinhdlparticlesizeandconcentrationchangesinsepticshockpatients AT jennyferyongsang highdensitylipoproteinhdlparticlesizeandconcentrationchangesinsepticshockpatients AT jessicapatche highdensitylipoproteinhdlparticlesizeandconcentrationchangesinsepticshockpatients AT anatoleharrois highdensitylipoproteinhdlparticlesizeandconcentrationchangesinsepticshockpatients AT sophiehamada highdensitylipoproteinhdlparticlesizeandconcentrationchangesinsepticshockpatients AT erickdenamur highdensitylipoproteinhdlparticlesizeandconcentrationchangesinsepticshockpatients AT philippemontravers highdensitylipoproteinhdlparticlesizeandconcentrationchangesinsepticshockpatients AT jacquesduranteau highdensitylipoproteinhdlparticlesizeandconcentrationchangesinsepticshockpatients AT oliviermeilhac highdensitylipoproteinhdlparticlesizeandconcentrationchangesinsepticshockpatients |
_version_ |
1724888823576395776 |