Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles
Abstract It is well known that pancreatic recovery after a single episode of injury such as an isolated bout of pancreatitis occurs rapidly. It is unclear, however, what changes are inflicted in such conditions to the molecular landscape of the pancreas. In the caerulein hyperstimulation model of pa...
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doaj-69e80f3f1fcb461aaae46aed4750a6cc2020-12-08T03:52:38ZengNature Publishing GroupScientific Reports2045-23222018-01-018111210.1038/s41598-018-19392-0Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profilesKristy Boggs0Ting Wang1Abrahim I. Orabi2Amitava Mukherjee3John F. Eisses4Tao Sun5Li Wen6Tanveer A. Javed7Farzad Esni8Wei Chen9Sohail Z. Husain10Department of Pediatrics, School of Medicine, University of PittsburghDepartment of Pediatrics, School of Medicine, University of PittsburghDepartment of Pediatrics, School of Medicine, University of PittsburghDepartment of Pediatrics, School of Medicine, University of PittsburghDepartment of Pediatrics, School of Medicine, University of PittsburghDepartment of Biostatistics, School of Public Health, University of PittsburghDepartment of Pediatrics, School of Medicine, University of PittsburghDepartment of Pediatrics, School of Medicine, University of PittsburghDepartment of Surgery, School of Medicine, University of PittsburghDepartment of Pediatrics, School of Medicine, University of PittsburghDepartment of Pediatrics, School of Medicine, University of PittsburghAbstract It is well known that pancreatic recovery after a single episode of injury such as an isolated bout of pancreatitis occurs rapidly. It is unclear, however, what changes are inflicted in such conditions to the molecular landscape of the pancreas. In the caerulein hyperstimulation model of pancreatitis, the murine pancreas has the ability to recover within one week based on histological appearance. In this study, we sought to characterize by RNA-sequencing (RNA-seq) the transcriptional profile of the recovering pancreas up to two weeks post-injury. We found that one week after injury there were 319 differentially expressed genes (DEGs) compared with baseline and that after two weeks there were 53 DEGs. Forty (12.5%) of the DEGs persisted from week one to week two, and another 13 DEGs newly emerged in the second week. Amongst the top up-regulated DEGs were several trypsinogen genes (trypsinogen 4, 5, 12, 15, and 16). To our knowledge, this is the first characterization of the transcriptome during pancreatic recovery by deep sequencing, and it reveals on a molecular basis that there is an ongoing recovery of the pancreas even after apparent histological resolution. The findings also raise the possibility of an emerging novel transcriptome upon pancreatic recovery.https://doi.org/10.1038/s41598-018-19392-0 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kristy Boggs Ting Wang Abrahim I. Orabi Amitava Mukherjee John F. Eisses Tao Sun Li Wen Tanveer A. Javed Farzad Esni Wei Chen Sohail Z. Husain |
spellingShingle |
Kristy Boggs Ting Wang Abrahim I. Orabi Amitava Mukherjee John F. Eisses Tao Sun Li Wen Tanveer A. Javed Farzad Esni Wei Chen Sohail Z. Husain Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles Scientific Reports |
author_facet |
Kristy Boggs Ting Wang Abrahim I. Orabi Amitava Mukherjee John F. Eisses Tao Sun Li Wen Tanveer A. Javed Farzad Esni Wei Chen Sohail Z. Husain |
author_sort |
Kristy Boggs |
title |
Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles |
title_short |
Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles |
title_full |
Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles |
title_fullStr |
Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles |
title_full_unstemmed |
Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles |
title_sort |
pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2018-01-01 |
description |
Abstract It is well known that pancreatic recovery after a single episode of injury such as an isolated bout of pancreatitis occurs rapidly. It is unclear, however, what changes are inflicted in such conditions to the molecular landscape of the pancreas. In the caerulein hyperstimulation model of pancreatitis, the murine pancreas has the ability to recover within one week based on histological appearance. In this study, we sought to characterize by RNA-sequencing (RNA-seq) the transcriptional profile of the recovering pancreas up to two weeks post-injury. We found that one week after injury there were 319 differentially expressed genes (DEGs) compared with baseline and that after two weeks there were 53 DEGs. Forty (12.5%) of the DEGs persisted from week one to week two, and another 13 DEGs newly emerged in the second week. Amongst the top up-regulated DEGs were several trypsinogen genes (trypsinogen 4, 5, 12, 15, and 16). To our knowledge, this is the first characterization of the transcriptome during pancreatic recovery by deep sequencing, and it reveals on a molecular basis that there is an ongoing recovery of the pancreas even after apparent histological resolution. The findings also raise the possibility of an emerging novel transcriptome upon pancreatic recovery. |
url |
https://doi.org/10.1038/s41598-018-19392-0 |
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