<it>In vitro</it> infection with classical swine fever virus inhibits the transcription of immune response genes
<p>Abstract</p> <p>Background</p> <p>Classical swine fever virus (CSFV) can evade the immune response and establish chronic infection under natural and experimental conditions. Some genes related to antigen processing and presentation and to cytokine regulation are know...
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doaj-69ec9d52de164d3884f1ec7596d07ef02020-11-24T22:22:36ZengBMCVirology Journal1743-422X2012-08-019117510.1186/1743-422X-9-175<it>In vitro</it> infection with classical swine fever virus inhibits the transcription of immune response genesFeng LiLi Xiao-QuanLi Xiao-ningLi JunMeng Xian-MingZhang Hong-YunLiang Jing-JingLi HuiSun Shi-KaiCai Xin-BinSu Li-JuanYin ShanLi Yan-ShengLuo Ting<p>Abstract</p> <p>Background</p> <p>Classical swine fever virus (CSFV) can evade the immune response and establish chronic infection under natural and experimental conditions. Some genes related to antigen processing and presentation and to cytokine regulation are known to be involved in this response, but the precise mechanism through which each gene responds to CSFV infection remains unclear.</p> <p>Results</p> <p>In this study, the amplification standard curve and corresponding linear regression equations for the genes SLA-2, TAP1, SLA-DR, Ii, CD40, CD80, CD86, IFN-α, and IFN-β were established successfully. Real-time RT-PCR was used to quantify the immune response gene transcription in PK-15 cells post CSFV infection. Results showed that: (1) immune response genes were generally down-regulated as a result of CSFV infection, and (2) the expression of SLA-2, SLA-DR, Ii and CD80 was significantly decreased (p<0.001).</p> <p>Conclusion</p> <p>We conclude that in vitro infection with CSFV inhibits the transcription of host immune response genes. These findings may facilitate the development of effective strategies for controlling CSF.</p> http://www.virologyj.com/content/9/1/175Classical swine fever virus (CSFV)Immune response genesReal-time RT-PCR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Feng Li Li Xiao-Quan Li Xiao-ning Li Jun Meng Xian-Ming Zhang Hong-Yun Liang Jing-Jing Li Hui Sun Shi-Kai Cai Xin-Bin Su Li-Juan Yin Shan Li Yan-Sheng Luo Ting |
spellingShingle |
Feng Li Li Xiao-Quan Li Xiao-ning Li Jun Meng Xian-Ming Zhang Hong-Yun Liang Jing-Jing Li Hui Sun Shi-Kai Cai Xin-Bin Su Li-Juan Yin Shan Li Yan-Sheng Luo Ting <it>In vitro</it> infection with classical swine fever virus inhibits the transcription of immune response genes Virology Journal Classical swine fever virus (CSFV) Immune response genes Real-time RT-PCR |
author_facet |
Feng Li Li Xiao-Quan Li Xiao-ning Li Jun Meng Xian-Ming Zhang Hong-Yun Liang Jing-Jing Li Hui Sun Shi-Kai Cai Xin-Bin Su Li-Juan Yin Shan Li Yan-Sheng Luo Ting |
author_sort |
Feng Li |
title |
<it>In vitro</it> infection with classical swine fever virus inhibits the transcription of immune response genes |
title_short |
<it>In vitro</it> infection with classical swine fever virus inhibits the transcription of immune response genes |
title_full |
<it>In vitro</it> infection with classical swine fever virus inhibits the transcription of immune response genes |
title_fullStr |
<it>In vitro</it> infection with classical swine fever virus inhibits the transcription of immune response genes |
title_full_unstemmed |
<it>In vitro</it> infection with classical swine fever virus inhibits the transcription of immune response genes |
title_sort |
<it>in vitro</it> infection with classical swine fever virus inhibits the transcription of immune response genes |
publisher |
BMC |
series |
Virology Journal |
issn |
1743-422X |
publishDate |
2012-08-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Classical swine fever virus (CSFV) can evade the immune response and establish chronic infection under natural and experimental conditions. Some genes related to antigen processing and presentation and to cytokine regulation are known to be involved in this response, but the precise mechanism through which each gene responds to CSFV infection remains unclear.</p> <p>Results</p> <p>In this study, the amplification standard curve and corresponding linear regression equations for the genes SLA-2, TAP1, SLA-DR, Ii, CD40, CD80, CD86, IFN-α, and IFN-β were established successfully. Real-time RT-PCR was used to quantify the immune response gene transcription in PK-15 cells post CSFV infection. Results showed that: (1) immune response genes were generally down-regulated as a result of CSFV infection, and (2) the expression of SLA-2, SLA-DR, Ii and CD80 was significantly decreased (p<0.001).</p> <p>Conclusion</p> <p>We conclude that in vitro infection with CSFV inhibits the transcription of host immune response genes. These findings may facilitate the development of effective strategies for controlling CSF.</p> |
topic |
Classical swine fever virus (CSFV) Immune response genes Real-time RT-PCR |
url |
http://www.virologyj.com/content/9/1/175 |
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