Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM
We describe an approach to non-invasively map spatiotemporal biochemical and physiological changes in 3D cell culture using Forster Resonance Energy Transfer (FRET) biosensors expressed in tumour spheroids. In particular, we present an improved Adenosine Monophosphate (AMP) Activated Protein Kinase...
Main Authors: | George Chennell, Robin J. W. Willows, Sean C. Warren, David Carling, Paul M. W. French, Chris Dunsby, Alessandro Sardini |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2016-08-01
|
Series: | Sensors |
Subjects: | |
Online Access: | http://www.mdpi.com/1424-8220/16/8/1312 |
Similar Items
-
Single-Cell Biochemical Multiplexing by Multidimensional Phasor Demixing and Spectral Fluorescence Lifetime Imaging Microscopy
by: Kalina T. Haas, et al.
Published: (2021-05-01) -
Quantitative FLIM-FRET Measurement of Voltage Dependent Prestin Conformational Changes
by: Mooney, Chance
Published: (2013) -
Fast Timing Techniques in FLIM Applications
by: Liisa M. Hirvonen, et al.
Published: (2020-05-01) -
Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM
by: Gregor P. C. Drummen, et al.
Published: (2012-04-01) -
Optimizing metastatic-cascade-dependent Rac1 targeting in breast cancer: Guidance using optical window intravital FRET imaging
by: Alessia Floerchinger, et al.
Published: (2021-09-01)