Hyperspectral Imagery for Assessing Laser-Induced Thermal State Change in Liver

This work presents the potential of hyperspectral imaging (HSI) to monitor the thermal outcome of laser ablation therapy used for minimally invasive tumor removal. Our main goal is the establishment of indicators of the thermal damage of living tissues, which can be used to assess the effect of the...

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Main Authors: Martina De Landro, Ignacio Espíritu García-Molina, Manuel Barberio, Eric Felli, Vincent Agnus, Margherita Pizzicannella, Michele Diana, Emanuele Zappa, Paola Saccomandi
Format: Article
Language:English
Published: MDPI AG 2021-01-01
Series:Sensors
Subjects:
Online Access:https://www.mdpi.com/1424-8220/21/2/643
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spelling doaj-69f5ea0b960e450290765670ee3e71b92021-01-19T00:04:23ZengMDPI AGSensors1424-82202021-01-012164364310.3390/s21020643Hyperspectral Imagery for Assessing Laser-Induced Thermal State Change in LiverMartina De Landro0Ignacio Espíritu García-Molina1Manuel Barberio2Eric Felli3Vincent Agnus4Margherita Pizzicannella5Michele Diana6Emanuele Zappa7Paola Saccomandi8Department of Mechanical Engineering, Politecnico di Milano, 20156 Milan, ItalyDepartment of Mechanical Engineering, Politecnico di Milano, 20156 Milan, ItalyIHU-Strasbourg, 67000 Strasbourg, FranceIHU-Strasbourg, 67000 Strasbourg, FranceIHU-Strasbourg, 67000 Strasbourg, FranceIHU-Strasbourg, 67000 Strasbourg, FranceIHU-Strasbourg, 67000 Strasbourg, FranceDepartment of Mechanical Engineering, Politecnico di Milano, 20156 Milan, ItalyDepartment of Mechanical Engineering, Politecnico di Milano, 20156 Milan, ItalyThis work presents the potential of hyperspectral imaging (HSI) to monitor the thermal outcome of laser ablation therapy used for minimally invasive tumor removal. Our main goal is the establishment of indicators of the thermal damage of living tissues, which can be used to assess the effect of the procedure. These indicators rely on the spectral variation of temperature-dependent tissue chromophores, i.e., oxyhemoglobin, deoxyhemoglobin, methemoglobin, and water. Laser treatment was performed at specific temperature thresholds (from 60 to 110 °C) on in-vivo animal liver and was assessed with a hyperspectral camera (500–995 nm) during and after the treatment. The indicators were extracted from the hyperspectral images after the following processing steps: the breathing motion compensation and the spectral and spatial filtering, the selection of spectral bands corresponding to specific tissue chromophores, and the analysis of the areas under the curves for each spectral band. Results show that properly combining spectral information related to deoxyhemoglobin, methemoglobin, lipids, and water allows for the segmenting of different zones of the laser-induced thermal damage. This preliminary investigation provides indicators for describing the thermal state of the liver, which can be employed in the future as clinical endpoints of the procedure outcome.https://www.mdpi.com/1424-8220/21/2/643Hyperspectral imagingimage processingimage segmentationlaser ablationtissue chromophoresthermal damage
collection DOAJ
language English
format Article
sources DOAJ
author Martina De Landro
Ignacio Espíritu García-Molina
Manuel Barberio
Eric Felli
Vincent Agnus
Margherita Pizzicannella
Michele Diana
Emanuele Zappa
Paola Saccomandi
spellingShingle Martina De Landro
Ignacio Espíritu García-Molina
Manuel Barberio
Eric Felli
Vincent Agnus
Margherita Pizzicannella
Michele Diana
Emanuele Zappa
Paola Saccomandi
Hyperspectral Imagery for Assessing Laser-Induced Thermal State Change in Liver
Sensors
Hyperspectral imaging
image processing
image segmentation
laser ablation
tissue chromophores
thermal damage
author_facet Martina De Landro
Ignacio Espíritu García-Molina
Manuel Barberio
Eric Felli
Vincent Agnus
Margherita Pizzicannella
Michele Diana
Emanuele Zappa
Paola Saccomandi
author_sort Martina De Landro
title Hyperspectral Imagery for Assessing Laser-Induced Thermal State Change in Liver
title_short Hyperspectral Imagery for Assessing Laser-Induced Thermal State Change in Liver
title_full Hyperspectral Imagery for Assessing Laser-Induced Thermal State Change in Liver
title_fullStr Hyperspectral Imagery for Assessing Laser-Induced Thermal State Change in Liver
title_full_unstemmed Hyperspectral Imagery for Assessing Laser-Induced Thermal State Change in Liver
title_sort hyperspectral imagery for assessing laser-induced thermal state change in liver
publisher MDPI AG
series Sensors
issn 1424-8220
publishDate 2021-01-01
description This work presents the potential of hyperspectral imaging (HSI) to monitor the thermal outcome of laser ablation therapy used for minimally invasive tumor removal. Our main goal is the establishment of indicators of the thermal damage of living tissues, which can be used to assess the effect of the procedure. These indicators rely on the spectral variation of temperature-dependent tissue chromophores, i.e., oxyhemoglobin, deoxyhemoglobin, methemoglobin, and water. Laser treatment was performed at specific temperature thresholds (from 60 to 110 °C) on in-vivo animal liver and was assessed with a hyperspectral camera (500–995 nm) during and after the treatment. The indicators were extracted from the hyperspectral images after the following processing steps: the breathing motion compensation and the spectral and spatial filtering, the selection of spectral bands corresponding to specific tissue chromophores, and the analysis of the areas under the curves for each spectral band. Results show that properly combining spectral information related to deoxyhemoglobin, methemoglobin, lipids, and water allows for the segmenting of different zones of the laser-induced thermal damage. This preliminary investigation provides indicators for describing the thermal state of the liver, which can be employed in the future as clinical endpoints of the procedure outcome.
topic Hyperspectral imaging
image processing
image segmentation
laser ablation
tissue chromophores
thermal damage
url https://www.mdpi.com/1424-8220/21/2/643
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