Effect of milrinone on the inflammatory response and NF-kB activation in renal ischemia-reperfusion injury in mice
BackgroundMilrinone increases intracellular adenosine 3',5'-cyclic monophosphate concentration and enhances vascular relaxation. Nuclear factor-kappa B (NF-kB) plays a key role in inflammatory responses during ischemia-reperfusion (I/R) injury. We aimed to investigate the effect of milrino...
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doaj-6a0abd7e0fe848a4a2d80f8baad87fae2020-11-25T03:57:44ZengKorean Society of AnesthesiologistsKorean Journal of Anesthesiology2005-64192005-75632014-02-0166213614210.4097/kjae.2014.66.2.1367816Effect of milrinone on the inflammatory response and NF-kB activation in renal ischemia-reperfusion injury in miceHong Soo Jung0Jin-Deok Joo1Dae-Woo Kim2Jang Hyeok In3Misun Roh4Jong-Tae Jeong5Seung June Noh6Jin Woo Choi7Department of Anesthesiology and Pain Medicine, College of Medicine, St. Vincent's Hospital, The Catholic University of Korea, Suwon, Korea.Department of Anesthesiology and Pain Medicine, College of Medicine, St. Vincent's Hospital, The Catholic University of Korea, Suwon, Korea.Department of Anesthesiology and Pain Medicine, College of Medicine, St. Vincent's Hospital, The Catholic University of Korea, Suwon, Korea.Department of Anesthesiology and Pain Medicine, College of Medicine, St. Vincent's Hospital, The Catholic University of Korea, Suwon, Korea.Department of Anesthesiology and Pain Medicine, College of Medicine, St. Vincent's Hospital, The Catholic University of Korea, Suwon, Korea.Department of Anesthesiology and Pain Medicine, College of Medicine, St. Vincent's Hospital, The Catholic University of Korea, Suwon, Korea.The Research Institute of Medical Science, St. Vincent's Hospital, The Catholic University of Korea, Suwon, Korea.Department of Anesthesiology and Pain Medicine, College of Medicine, St. Vincent's Hospital, The Catholic University of Korea, Suwon, Korea.BackgroundMilrinone increases intracellular adenosine 3',5'-cyclic monophosphate concentration and enhances vascular relaxation. Nuclear factor-kappa B (NF-kB) plays a key role in inflammatory responses during ischemia-reperfusion (I/R) injury. We aimed to investigate the effect of milrinone on the inflammatory responses and NF-kB activation in renal I/R injury in mice.MethodsThirty C57BL/6 mice were allocated into 3 groups. In group S (n = 10), only right nephrectomy was done. In group C (n = 10), the left kidney was subjected to 30 min of ischemia after right nephrectomy. In group M (n = 10), milrinone (5 µg/kg) was administered before ischemia. After 24 hours of reperfusion, the serum creatinine was measured, kidney samples were obtained for histology, and expressions of NF-kB and proinflammatory cytokines were analyzed.ResultsIn group C, the serum creatinine concentration was markedly elevated, compared with group S. Creatinine concentration in group M was also elevated, but it was significantly lower than that in group C. Histologic evidence of renal damage was severe in group C, but it was improved in group M. In groups C and M, expression of NF-kB, tumor necrosis factor-α (TNF-α), intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-2 (MIP-2) mRNA increased significantly compared with group S (P < 0.05). But group M showed a lower expression of NF-kB, TNF-α, ICAM-1, MCP-1 and MIP-2 mRNA than group C (P < 0.05).ConclusionsMilrinone treatment attenuates the renal inflammatory response and activation of NF-kB, resulting in improvement of renal function and tissue injury.http://ekja.org/upload/pdf/kjae-66-136.pdfischemia-reperfusion injurymilrinonenuclear-factor-kappa b |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hong Soo Jung Jin-Deok Joo Dae-Woo Kim Jang Hyeok In Misun Roh Jong-Tae Jeong Seung June Noh Jin Woo Choi |
spellingShingle |
Hong Soo Jung Jin-Deok Joo Dae-Woo Kim Jang Hyeok In Misun Roh Jong-Tae Jeong Seung June Noh Jin Woo Choi Effect of milrinone on the inflammatory response and NF-kB activation in renal ischemia-reperfusion injury in mice Korean Journal of Anesthesiology ischemia-reperfusion injury milrinone nuclear-factor-kappa b |
author_facet |
Hong Soo Jung Jin-Deok Joo Dae-Woo Kim Jang Hyeok In Misun Roh Jong-Tae Jeong Seung June Noh Jin Woo Choi |
author_sort |
Hong Soo Jung |
title |
Effect of milrinone on the inflammatory response and NF-kB activation in renal ischemia-reperfusion injury in mice |
title_short |
Effect of milrinone on the inflammatory response and NF-kB activation in renal ischemia-reperfusion injury in mice |
title_full |
Effect of milrinone on the inflammatory response and NF-kB activation in renal ischemia-reperfusion injury in mice |
title_fullStr |
Effect of milrinone on the inflammatory response and NF-kB activation in renal ischemia-reperfusion injury in mice |
title_full_unstemmed |
Effect of milrinone on the inflammatory response and NF-kB activation in renal ischemia-reperfusion injury in mice |
title_sort |
effect of milrinone on the inflammatory response and nf-kb activation in renal ischemia-reperfusion injury in mice |
publisher |
Korean Society of Anesthesiologists |
series |
Korean Journal of Anesthesiology |
issn |
2005-6419 2005-7563 |
publishDate |
2014-02-01 |
description |
BackgroundMilrinone increases intracellular adenosine 3',5'-cyclic monophosphate concentration and enhances vascular relaxation. Nuclear factor-kappa B (NF-kB) plays a key role in inflammatory responses during ischemia-reperfusion (I/R) injury. We aimed to investigate the effect of milrinone on the inflammatory responses and NF-kB activation in renal I/R injury in mice.MethodsThirty C57BL/6 mice were allocated into 3 groups. In group S (n = 10), only right nephrectomy was done. In group C (n = 10), the left kidney was subjected to 30 min of ischemia after right nephrectomy. In group M (n = 10), milrinone (5 µg/kg) was administered before ischemia. After 24 hours of reperfusion, the serum creatinine was measured, kidney samples were obtained for histology, and expressions of NF-kB and proinflammatory cytokines were analyzed.ResultsIn group C, the serum creatinine concentration was markedly elevated, compared with group S. Creatinine concentration in group M was also elevated, but it was significantly lower than that in group C. Histologic evidence of renal damage was severe in group C, but it was improved in group M. In groups C and M, expression of NF-kB, tumor necrosis factor-α (TNF-α), intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-2 (MIP-2) mRNA increased significantly compared with group S (P < 0.05). But group M showed a lower expression of NF-kB, TNF-α, ICAM-1, MCP-1 and MIP-2 mRNA than group C (P < 0.05).ConclusionsMilrinone treatment attenuates the renal inflammatory response and activation of NF-kB, resulting in improvement of renal function and tissue injury. |
topic |
ischemia-reperfusion injury milrinone nuclear-factor-kappa b |
url |
http://ekja.org/upload/pdf/kjae-66-136.pdf |
work_keys_str_mv |
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