Robust detection of rare species using environmental DNA: the importance of primer specificity.

Environmental DNA (eDNA) is being rapidly adopted as a tool to detect rare animals. Quantitative PCR (qPCR) using probe-based chemistries may represent a particularly powerful tool because of the method's sensitivity, specificity, and potential to quantify target DNA. However, there has been li...

Full description

Bibliographic Details
Main Authors: Taylor M Wilcox, Kevin S McKelvey, Michael K Young, Stephen F Jane, Winsor H Lowe, Andrew R Whiteley, Michael K Schwartz
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3608683?pdf=render