Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia
Acellular vaccines against Bordetella pertussis were introduced in Australia in 1997. By 2000, these vaccines had replaced whole-cell vaccines. During 2008–2012, a large outbreak of pertussis occurred. During this period, 30% (96/320) of B. pertussis isolates did not express the vaccine antigen pert...
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2014-04-01
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doaj-6a2ac41948fe4318befa5a13f08b99782020-11-24T21:44:22ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592014-04-0120462663310.3201/eid2004.131478Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, AustraliaConnie LamSophie OctaviaLawrence RicafortVitali SintchenkoGwendolyn L. GilbertNicholas WoodPeter McIntyreHelen MarshallNicole GuisoAnthony D. KeilAndrew LawrenceJenny RobsonGeoff HoggRuiting LanAcellular vaccines against Bordetella pertussis were introduced in Australia in 1997. By 2000, these vaccines had replaced whole-cell vaccines. During 2008–2012, a large outbreak of pertussis occurred. During this period, 30% (96/320) of B. pertussis isolates did not express the vaccine antigen pertactin (prn). Multiple mechanisms of prn inactivation were documented, including IS481 and IS1002 disruptions, a variation within a homopolymeric tract, and deletion of the prn gene. The mechanism of lack of expression of prn in 16 (17%) isolates could not be determined at the sequence level. These findings suggest that B. pertussis not expressing prn arose independently multiple times since 2008, rather than by expansion of a single prn-negative clone. All but 1 isolate had ptxA1, prn2, and ptxP3, the alleles representative of currently circulating strains in Australia. This pattern is consistent with continuing evolution of B. pertussis in response to vaccine selection pressure.https://wwwnc.cdc.gov/eid/article/20/4/13-1478_articleBordetella pertussisbacteriapertactinevolutionvaccineAustralia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Connie Lam Sophie Octavia Lawrence Ricafort Vitali Sintchenko Gwendolyn L. Gilbert Nicholas Wood Peter McIntyre Helen Marshall Nicole Guiso Anthony D. Keil Andrew Lawrence Jenny Robson Geoff Hogg Ruiting Lan |
spellingShingle |
Connie Lam Sophie Octavia Lawrence Ricafort Vitali Sintchenko Gwendolyn L. Gilbert Nicholas Wood Peter McIntyre Helen Marshall Nicole Guiso Anthony D. Keil Andrew Lawrence Jenny Robson Geoff Hogg Ruiting Lan Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia Emerging Infectious Diseases Bordetella pertussis bacteria pertactin evolution vaccine Australia |
author_facet |
Connie Lam Sophie Octavia Lawrence Ricafort Vitali Sintchenko Gwendolyn L. Gilbert Nicholas Wood Peter McIntyre Helen Marshall Nicole Guiso Anthony D. Keil Andrew Lawrence Jenny Robson Geoff Hogg Ruiting Lan |
author_sort |
Connie Lam |
title |
Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia |
title_short |
Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia |
title_full |
Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia |
title_fullStr |
Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia |
title_full_unstemmed |
Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia |
title_sort |
rapid increase in pertactin-deficient bordetella pertussis isolates, australia |
publisher |
Centers for Disease Control and Prevention |
series |
Emerging Infectious Diseases |
issn |
1080-6040 1080-6059 |
publishDate |
2014-04-01 |
description |
Acellular vaccines against Bordetella pertussis were introduced in Australia in 1997. By 2000, these vaccines had replaced whole-cell vaccines. During 2008–2012, a large outbreak of pertussis occurred. During this period, 30% (96/320) of B. pertussis isolates did not express the vaccine antigen pertactin (prn). Multiple mechanisms of prn inactivation were documented, including IS481 and IS1002 disruptions, a variation within a homopolymeric tract, and deletion of the prn gene. The mechanism of lack of expression of prn in 16 (17%) isolates could not be determined at the sequence level. These findings suggest that B. pertussis not expressing prn arose independently multiple times since 2008, rather than by expansion of a single prn-negative clone. All but 1 isolate had ptxA1, prn2, and ptxP3, the alleles representative of currently circulating strains in Australia. This pattern is consistent with continuing evolution of B. pertussis in response to vaccine selection pressure. |
topic |
Bordetella pertussis bacteria pertactin evolution vaccine Australia |
url |
https://wwwnc.cdc.gov/eid/article/20/4/13-1478_article |
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