Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia

Acellular vaccines against Bordetella pertussis were introduced in Australia in 1997. By 2000, these vaccines had replaced whole-cell vaccines. During 2008–2012, a large outbreak of pertussis occurred. During this period, 30% (96/320) of B. pertussis isolates did not express the vaccine antigen pert...

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Main Authors: Connie Lam, Sophie Octavia, Lawrence Ricafort, Vitali Sintchenko, Gwendolyn L. Gilbert, Nicholas Wood, Peter McIntyre, Helen Marshall, Nicole Guiso, Anthony D. Keil, Andrew Lawrence, Jenny Robson, Geoff Hogg, Ruiting Lan
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2014-04-01
Series:Emerging Infectious Diseases
Subjects:
Online Access:https://wwwnc.cdc.gov/eid/article/20/4/13-1478_article
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spelling doaj-6a2ac41948fe4318befa5a13f08b99782020-11-24T21:44:22ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592014-04-0120462663310.3201/eid2004.131478Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, AustraliaConnie LamSophie OctaviaLawrence RicafortVitali SintchenkoGwendolyn L. GilbertNicholas WoodPeter McIntyreHelen MarshallNicole GuisoAnthony D. KeilAndrew LawrenceJenny RobsonGeoff HoggRuiting LanAcellular vaccines against Bordetella pertussis were introduced in Australia in 1997. By 2000, these vaccines had replaced whole-cell vaccines. During 2008–2012, a large outbreak of pertussis occurred. During this period, 30% (96/320) of B. pertussis isolates did not express the vaccine antigen pertactin (prn). Multiple mechanisms of prn inactivation were documented, including IS481 and IS1002 disruptions, a variation within a homopolymeric tract, and deletion of the prn gene. The mechanism of lack of expression of prn in 16 (17%) isolates could not be determined at the sequence level. These findings suggest that B. pertussis not expressing prn arose independently multiple times since 2008, rather than by expansion of a single prn-negative clone. All but 1 isolate had ptxA1, prn2, and ptxP3, the alleles representative of currently circulating strains in Australia. This pattern is consistent with continuing evolution of B. pertussis in response to vaccine selection pressure.https://wwwnc.cdc.gov/eid/article/20/4/13-1478_articleBordetella pertussisbacteriapertactinevolutionvaccineAustralia
collection DOAJ
language English
format Article
sources DOAJ
author Connie Lam
Sophie Octavia
Lawrence Ricafort
Vitali Sintchenko
Gwendolyn L. Gilbert
Nicholas Wood
Peter McIntyre
Helen Marshall
Nicole Guiso
Anthony D. Keil
Andrew Lawrence
Jenny Robson
Geoff Hogg
Ruiting Lan
spellingShingle Connie Lam
Sophie Octavia
Lawrence Ricafort
Vitali Sintchenko
Gwendolyn L. Gilbert
Nicholas Wood
Peter McIntyre
Helen Marshall
Nicole Guiso
Anthony D. Keil
Andrew Lawrence
Jenny Robson
Geoff Hogg
Ruiting Lan
Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia
Emerging Infectious Diseases
Bordetella pertussis
bacteria
pertactin
evolution
vaccine
Australia
author_facet Connie Lam
Sophie Octavia
Lawrence Ricafort
Vitali Sintchenko
Gwendolyn L. Gilbert
Nicholas Wood
Peter McIntyre
Helen Marshall
Nicole Guiso
Anthony D. Keil
Andrew Lawrence
Jenny Robson
Geoff Hogg
Ruiting Lan
author_sort Connie Lam
title Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia
title_short Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia
title_full Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia
title_fullStr Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia
title_full_unstemmed Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia
title_sort rapid increase in pertactin-deficient bordetella pertussis isolates, australia
publisher Centers for Disease Control and Prevention
series Emerging Infectious Diseases
issn 1080-6040
1080-6059
publishDate 2014-04-01
description Acellular vaccines against Bordetella pertussis were introduced in Australia in 1997. By 2000, these vaccines had replaced whole-cell vaccines. During 2008–2012, a large outbreak of pertussis occurred. During this period, 30% (96/320) of B. pertussis isolates did not express the vaccine antigen pertactin (prn). Multiple mechanisms of prn inactivation were documented, including IS481 and IS1002 disruptions, a variation within a homopolymeric tract, and deletion of the prn gene. The mechanism of lack of expression of prn in 16 (17%) isolates could not be determined at the sequence level. These findings suggest that B. pertussis not expressing prn arose independently multiple times since 2008, rather than by expansion of a single prn-negative clone. All but 1 isolate had ptxA1, prn2, and ptxP3, the alleles representative of currently circulating strains in Australia. This pattern is consistent with continuing evolution of B. pertussis in response to vaccine selection pressure.
topic Bordetella pertussis
bacteria
pertactin
evolution
vaccine
Australia
url https://wwwnc.cdc.gov/eid/article/20/4/13-1478_article
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