Porcine circovirus 2 (PCV2) increases the expression of endothelial adhesion/junction molecules
Abstract Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus disease, a complex multisystem syndrome in domestic pigs. Despite the significant economic losses caused by porcine circovirus disease, the mechanisms of pathogenesis underlying the clinical findings remai...
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doaj-6a3d9d8443b24dd2805695116f3593202020-11-24T23:08:58ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1678-440547487087510.1016/j.bjm.2016.07.001S1517-83822016000400870Porcine circovirus 2 (PCV2) increases the expression of endothelial adhesion/junction moleculesFernanda S. MarksLaura L. AlmeidaDavid DriemeierCláudio CanalDavid E.S.N. BarcellosJorge A. GuimarãesJosé ReckAbstract Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus disease, a complex multisystem syndrome in domestic pigs. Despite the significant economic losses caused by porcine circovirus disease, the mechanisms of pathogenesis underlying the clinical findings remain largely unclear. As various reports have highlighted the potential key role of vascular lesions in the pathogenesis of porcine circovirus disease, the aim of this work was to investigate effects of PCV2 infection on vascular endothelial cells, focusing on cell viability and expression of adhesion/junction molecules. PCV2 infection reduced endothelial cell viability, while viral infection did not affected the viability of several other classical cell lines. Also, PCV2 infection in endothelial cells displayed a dual/biphasic effect: initially, infection increased ICAM-1 expression, which can favor leukocyte recruitment and emigration to tissues and possibly inducing characteristic porcine circovirus disease inflammatory lesions; then, secondarily, infection caused an increase in zonula occludens 1 tight junction protein (ZO-1) expression, which in turn can result in difficulties for cell traffic across the endothelium and a potential impairment the immune response in peripheral tissues. These virus-induced endothelial changes could directly impact the inflammatory process of porcine circovirus disease and associated vascular/immune system disturbances. Data suggest that, among the wide range of effects induced by PCV2 on the host, endothelial modulation can be a pivotal process which can help to explain PCV2 pathogenesis in some porcine circovirus disease presentations.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400870&lng=en&tlng=enSwineInfectious diseasesPathogenesisViral infectionCell adhesion |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fernanda S. Marks Laura L. Almeida David Driemeier Cláudio Canal David E.S.N. Barcellos Jorge A. Guimarães José Reck |
spellingShingle |
Fernanda S. Marks Laura L. Almeida David Driemeier Cláudio Canal David E.S.N. Barcellos Jorge A. Guimarães José Reck Porcine circovirus 2 (PCV2) increases the expression of endothelial adhesion/junction molecules Brazilian Journal of Microbiology Swine Infectious diseases Pathogenesis Viral infection Cell adhesion |
author_facet |
Fernanda S. Marks Laura L. Almeida David Driemeier Cláudio Canal David E.S.N. Barcellos Jorge A. Guimarães José Reck |
author_sort |
Fernanda S. Marks |
title |
Porcine circovirus 2 (PCV2) increases the expression of endothelial adhesion/junction molecules |
title_short |
Porcine circovirus 2 (PCV2) increases the expression of endothelial adhesion/junction molecules |
title_full |
Porcine circovirus 2 (PCV2) increases the expression of endothelial adhesion/junction molecules |
title_fullStr |
Porcine circovirus 2 (PCV2) increases the expression of endothelial adhesion/junction molecules |
title_full_unstemmed |
Porcine circovirus 2 (PCV2) increases the expression of endothelial adhesion/junction molecules |
title_sort |
porcine circovirus 2 (pcv2) increases the expression of endothelial adhesion/junction molecules |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1678-4405 |
description |
Abstract Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus disease, a complex multisystem syndrome in domestic pigs. Despite the significant economic losses caused by porcine circovirus disease, the mechanisms of pathogenesis underlying the clinical findings remain largely unclear. As various reports have highlighted the potential key role of vascular lesions in the pathogenesis of porcine circovirus disease, the aim of this work was to investigate effects of PCV2 infection on vascular endothelial cells, focusing on cell viability and expression of adhesion/junction molecules. PCV2 infection reduced endothelial cell viability, while viral infection did not affected the viability of several other classical cell lines. Also, PCV2 infection in endothelial cells displayed a dual/biphasic effect: initially, infection increased ICAM-1 expression, which can favor leukocyte recruitment and emigration to tissues and possibly inducing characteristic porcine circovirus disease inflammatory lesions; then, secondarily, infection caused an increase in zonula occludens 1 tight junction protein (ZO-1) expression, which in turn can result in difficulties for cell traffic across the endothelium and a potential impairment the immune response in peripheral tissues. These virus-induced endothelial changes could directly impact the inflammatory process of porcine circovirus disease and associated vascular/immune system disturbances. Data suggest that, among the wide range of effects induced by PCV2 on the host, endothelial modulation can be a pivotal process which can help to explain PCV2 pathogenesis in some porcine circovirus disease presentations. |
topic |
Swine Infectious diseases Pathogenesis Viral infection Cell adhesion |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400870&lng=en&tlng=en |
work_keys_str_mv |
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