Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo

The present study was designed to investigate the combined effects of cyclooxygenase (COX)-1 and COX-2 selective inhibitors on human ovarian SKOV-3 carcinoma cells xenograft-bearing mice. The animals were treated with 3 mg/kg SC-560 (a COX-1 selective inhibitor) alone, 25 mg/kg celecoxib (a COX-2 se...

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Main Authors: Jun Zhang, Mei-Lin Liu, Ling-Yun Zhai, Hong-Ru Jiang, Xiao-Li Xu, Jie Wang, Wei Li
Format: Article
Language:English
Published: MDPI AG 2011-01-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:http://www.mdpi.com/1422-0067/12/1/668/
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spelling doaj-6a41172923084fceb69c66fd362c38162020-11-24T21:12:36ZengMDPI AGInternational Journal of Molecular Sciences1422-00672011-01-0112166868110.3390/ijms12010668Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in VivoJun ZhangMei-Lin LiuLing-Yun ZhaiHong-Ru JiangXiao-Li XuJie WangWei LiThe present study was designed to investigate the combined effects of cyclooxygenase (COX)-1 and COX-2 selective inhibitors on human ovarian SKOV-3 carcinoma cells xenograft-bearing mice. The animals were treated with 3 mg/kg SC-560 (a COX-1 selective inhibitor) alone, 25 mg/kg celecoxib (a COX-2 selective inhibitor) alone, or SC-560/celecoxib by gavage, twice a day for three weeks. To test the mechanism of inhibition of tumor growth by COX selective inhibitors, the index of proliferating cells in tumor tissues was determined by immunostaining and the index of apoptotic cells by the terminal-deoxynucleotidyl-transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) method. The inhibitory rate on tumor growth in the combination group was 35.54% which is significant statistically compared with that of the control group (P < 0.05). In the combination group, the index of cell proliferation and apoptosis were 12.40% and 51.03% respectively, which are significant statistically compared with those of the control group (22.56%, 19.07%, all P < 0.05). These studies indicate that synergism between two COX inhibitors and inhibitor combination treatment has particular potential for chemoprevention of ovarian cancer growth. http://www.mdpi.com/1422-0067/12/1/668/ovarian carcinomamicecyclooxygenase selective inhibitorcell proliferationapoptosis
collection DOAJ
language English
format Article
sources DOAJ
author Jun Zhang
Mei-Lin Liu
Ling-Yun Zhai
Hong-Ru Jiang
Xiao-Li Xu
Jie Wang
Wei Li
spellingShingle Jun Zhang
Mei-Lin Liu
Ling-Yun Zhai
Hong-Ru Jiang
Xiao-Li Xu
Jie Wang
Wei Li
Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo
International Journal of Molecular Sciences
ovarian carcinoma
mice
cyclooxygenase selective inhibitor
cell proliferation
apoptosis
author_facet Jun Zhang
Mei-Lin Liu
Ling-Yun Zhai
Hong-Ru Jiang
Xiao-Li Xu
Jie Wang
Wei Li
author_sort Jun Zhang
title Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo
title_short Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo
title_full Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo
title_fullStr Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo
title_full_unstemmed Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo
title_sort combined effects of cyclooxygenase-1 and cyclooxygenase-2 selective inhibitors on ovarian carcinoma in vivo
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2011-01-01
description The present study was designed to investigate the combined effects of cyclooxygenase (COX)-1 and COX-2 selective inhibitors on human ovarian SKOV-3 carcinoma cells xenograft-bearing mice. The animals were treated with 3 mg/kg SC-560 (a COX-1 selective inhibitor) alone, 25 mg/kg celecoxib (a COX-2 selective inhibitor) alone, or SC-560/celecoxib by gavage, twice a day for three weeks. To test the mechanism of inhibition of tumor growth by COX selective inhibitors, the index of proliferating cells in tumor tissues was determined by immunostaining and the index of apoptotic cells by the terminal-deoxynucleotidyl-transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) method. The inhibitory rate on tumor growth in the combination group was 35.54% which is significant statistically compared with that of the control group (P < 0.05). In the combination group, the index of cell proliferation and apoptosis were 12.40% and 51.03% respectively, which are significant statistically compared with those of the control group (22.56%, 19.07%, all P < 0.05). These studies indicate that synergism between two COX inhibitors and inhibitor combination treatment has particular potential for chemoprevention of ovarian cancer growth.
topic ovarian carcinoma
mice
cyclooxygenase selective inhibitor
cell proliferation
apoptosis
url http://www.mdpi.com/1422-0067/12/1/668/
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