Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment
Introduction: Obstructive sleep apnoea (OSA) is common in obesity and is associated with cardiovascular and metabolic complications. Continuous positive airway pressure (CPAP) in OSA may lead to physiological changes reflected in the urinary proteome. The aim of this study was to characterise the ur...
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2015-04-01
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doaj-6a43a7c325a146419e56822396467fb12020-11-24T22:39:47ZengBrazilian Association of Sleep and Latin American Federation of Sleep Societies Sleep Science1984-00632015-04-0182586710.1016/j.slsci.2015.06.004Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatmentIan W Seetho0Adela Ramírez-Torres1Amaya Albalat2William Mullen3Harald Mischak4Robert J Parker5Sonya Craig6Nick Duffy7Kevin J Hardy8Jatin G Burniston9John PH Wilding10Department of Obesity & Endocrinology, University of Liverpool, UKMosaiques Diagnostics GmbH, Hannover, GermanyBHF Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, UKBHF Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, UKBHF Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, UKDepartment of Respiratory Medicine, University Hospital Aintree, Liverpool, UKDepartment of Respiratory Medicine, University Hospital Aintree, Liverpool, UKDepartment of Respiratory Medicine, University Hospital Aintree, Liverpool, UKDepartment of Diabetes & Endocrinology, St Helens & Knowsley Teaching Hospitals, UKSchool of Sports & Exercise Sciences, Liverpool John Moores University, UKDepartment of Obesity & Endocrinology, University of Liverpool, UKIntroduction: Obstructive sleep apnoea (OSA) is common in obesity and is associated with cardiovascular and metabolic complications. Continuous positive airway pressure (CPAP) in OSA may lead to physiological changes reflected in the urinary proteome. The aim of this study was to characterise the urinary proteome in severely obese adult subjects with OSA who were receiving CPAP compared with severely obese subjects without OSA. Methods: Severely obese subjects with and without OSA were recruited. Subjects with OSA were receiving CPAP. Body composition and blood pressure measurements were recorded. Urinary samples were analysed by Capillary Electrophoresis–Mass Spectrometry (CE–MS). Results: Twenty-seven subjects with OSA-on-CPAP (age 49±7years, BMI 43±7 kg/m2) and 25 controls without OSA (age 52±9years, BMI 39±4 kg/m2) were studied. Age and BMI were not significantly different between groups. Mean CPAP use for OSA patients was 14.5±1.0 months. Metabolic syndrome was present in 14(52%) of those with OSA compared with 6(24%) of controls (p=0.039). A urinary proteome comprising 15 peptides was identified showing differential expression between the groups (p<0.01). Although correction for multiple testing did not reach significance, sequences were determined for 8 peptides demonstrating origins from collagens, fibrinogen beta chain and T-cadherin that may be associated with underlying cardiovascular disease mechanisms in OSA. Conclusions: The urinary proteome is compared in OSA with CPAP and without OSA in severe obesity. The effects of CPAP on OSA may lead to changes in the urinary peptides but further research work is needed to investigate the potential role for urinary proteomics in characterising urinary peptide profiles in OSA.http://www.sciencedirect.com/science/article/pii/S1984006315000383Obstructive sleep apnoeaSevere obesityUrinary proteomicsCPAP |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ian W Seetho Adela Ramírez-Torres Amaya Albalat William Mullen Harald Mischak Robert J Parker Sonya Craig Nick Duffy Kevin J Hardy Jatin G Burniston John PH Wilding |
spellingShingle |
Ian W Seetho Adela Ramírez-Torres Amaya Albalat William Mullen Harald Mischak Robert J Parker Sonya Craig Nick Duffy Kevin J Hardy Jatin G Burniston John PH Wilding Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment Sleep Science Obstructive sleep apnoea Severe obesity Urinary proteomics CPAP |
author_facet |
Ian W Seetho Adela Ramírez-Torres Amaya Albalat William Mullen Harald Mischak Robert J Parker Sonya Craig Nick Duffy Kevin J Hardy Jatin G Burniston John PH Wilding |
author_sort |
Ian W Seetho |
title |
Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment |
title_short |
Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment |
title_full |
Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment |
title_fullStr |
Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment |
title_full_unstemmed |
Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment |
title_sort |
urinary proteomic profiling in severe obesity and obstructive sleep apnoea with cpap treatment |
publisher |
Brazilian Association of Sleep and Latin American Federation of Sleep Societies |
series |
Sleep Science |
issn |
1984-0063 |
publishDate |
2015-04-01 |
description |
Introduction: Obstructive sleep apnoea (OSA) is common in obesity and is associated with cardiovascular and metabolic complications. Continuous positive airway pressure (CPAP) in OSA may lead to physiological changes reflected in the urinary proteome. The aim of this study was to characterise the urinary proteome in severely obese adult subjects with OSA who were receiving CPAP compared with severely obese subjects without OSA.
Methods: Severely obese subjects with and without OSA were recruited. Subjects with OSA were receiving CPAP. Body composition and blood pressure measurements were recorded. Urinary samples were analysed by Capillary Electrophoresis–Mass Spectrometry (CE–MS).
Results: Twenty-seven subjects with OSA-on-CPAP (age 49±7years, BMI 43±7 kg/m2) and 25 controls without OSA (age 52±9years, BMI 39±4 kg/m2) were studied. Age and BMI were not significantly different between groups. Mean CPAP use for OSA patients was 14.5±1.0 months. Metabolic syndrome was present in 14(52%) of those with OSA compared with 6(24%) of controls (p=0.039). A urinary proteome comprising 15 peptides was identified showing differential expression between the groups (p<0.01). Although correction for multiple testing did not reach significance, sequences were determined for 8 peptides demonstrating origins from collagens, fibrinogen beta chain and T-cadherin that may be associated with underlying cardiovascular disease mechanisms in OSA.
Conclusions: The urinary proteome is compared in OSA with CPAP and without OSA in severe obesity. The effects of CPAP on OSA may lead to changes in the urinary peptides but further research work is needed to investigate the potential role for urinary proteomics in characterising urinary peptide profiles in OSA. |
topic |
Obstructive sleep apnoea Severe obesity Urinary proteomics CPAP |
url |
http://www.sciencedirect.com/science/article/pii/S1984006315000383 |
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