Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus
Abstract Background Maize dwarf mosaic virus (MDMV), a member of the genus Potyvirus, infects maize and is non-persistently transmitted by aphids. Several plant viruses have been developed as tools for gene expression and gene silencing in plants. The capacity of MDMV for both gene expression and ge...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2021-05-01
|
Series: | BMC Plant Biology |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12870-021-02971-1 |
id |
doaj-6a559405793d4ffa8c51c4b6670055d3 |
---|---|
record_format |
Article |
spelling |
doaj-6a559405793d4ffa8c51c4b6670055d32021-05-09T11:17:23ZengBMCBMC Plant Biology1471-22292021-05-0121111710.1186/s12870-021-02971-1Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virusWenshuang Xie0Dee Marie Marty1Junhuan Xu2Nitika Khatri3Kristen Willie4Wanderson Bucker Moraes5Lucy R. Stewart6Department of Plant Pathology, Ohio State UniversityUSDA-ARS Corn Soybean and Wheat Quality Research UnitDepartment of Plant Pathology, Ohio State UniversityDepartment of Plant Pathology, Ohio State UniversityUSDA-ARS Corn Soybean and Wheat Quality Research UnitDepartment of Plant Pathology, Ohio State UniversityUSDA-ARS Corn Soybean and Wheat Quality Research UnitAbstract Background Maize dwarf mosaic virus (MDMV), a member of the genus Potyvirus, infects maize and is non-persistently transmitted by aphids. Several plant viruses have been developed as tools for gene expression and gene silencing in plants. The capacity of MDMV for both gene expression and gene silencing were examined. Results Infectious clones of an Ohio isolate of MDMV, MDMV OH5, were obtained, and engineered for gene expression only, and for simultaneous marker gene expression and virus-induced gene silencing (VIGS) of three endogenous maize target genes. Single gene expression in single insertion constructs and simultaneous expression of green fluorescent protein (GFP) and silencing of three maize genes in a double insertion construct was demonstrated. Constructs with GFP inserted in the N-terminus of HCPro were more stable than those with insertion at the N-terminus of CP in our study. Unexpectedly, the construct with two insertion sites also retained insertions at a higher rate than single-insertion constructs. Engineered MDMV expression and VIGS constructs were transmissible by aphids (Rhopalosiphum padi). Conclusions These results demonstrate that MDMV-based vector can be used as a tool for simultaneous gene expression and multi-gene silencing in maize.https://doi.org/10.1186/s12870-021-02971-1Gene expressionMultigene silencingPhotobleachingPotyvirusVascular puncture inoculation (VPI)Virus-induced gene silencing (VIGS) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wenshuang Xie Dee Marie Marty Junhuan Xu Nitika Khatri Kristen Willie Wanderson Bucker Moraes Lucy R. Stewart |
spellingShingle |
Wenshuang Xie Dee Marie Marty Junhuan Xu Nitika Khatri Kristen Willie Wanderson Bucker Moraes Lucy R. Stewart Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus BMC Plant Biology Gene expression Multigene silencing Photobleaching Potyvirus Vascular puncture inoculation (VPI) Virus-induced gene silencing (VIGS) |
author_facet |
Wenshuang Xie Dee Marie Marty Junhuan Xu Nitika Khatri Kristen Willie Wanderson Bucker Moraes Lucy R. Stewart |
author_sort |
Wenshuang Xie |
title |
Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus |
title_short |
Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus |
title_full |
Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus |
title_fullStr |
Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus |
title_full_unstemmed |
Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus |
title_sort |
simultaneous gene expression and multi-gene silencing in zea mays using maize dwarf mosaic virus |
publisher |
BMC |
series |
BMC Plant Biology |
issn |
1471-2229 |
publishDate |
2021-05-01 |
description |
Abstract Background Maize dwarf mosaic virus (MDMV), a member of the genus Potyvirus, infects maize and is non-persistently transmitted by aphids. Several plant viruses have been developed as tools for gene expression and gene silencing in plants. The capacity of MDMV for both gene expression and gene silencing were examined. Results Infectious clones of an Ohio isolate of MDMV, MDMV OH5, were obtained, and engineered for gene expression only, and for simultaneous marker gene expression and virus-induced gene silencing (VIGS) of three endogenous maize target genes. Single gene expression in single insertion constructs and simultaneous expression of green fluorescent protein (GFP) and silencing of three maize genes in a double insertion construct was demonstrated. Constructs with GFP inserted in the N-terminus of HCPro were more stable than those with insertion at the N-terminus of CP in our study. Unexpectedly, the construct with two insertion sites also retained insertions at a higher rate than single-insertion constructs. Engineered MDMV expression and VIGS constructs were transmissible by aphids (Rhopalosiphum padi). Conclusions These results demonstrate that MDMV-based vector can be used as a tool for simultaneous gene expression and multi-gene silencing in maize. |
topic |
Gene expression Multigene silencing Photobleaching Potyvirus Vascular puncture inoculation (VPI) Virus-induced gene silencing (VIGS) |
url |
https://doi.org/10.1186/s12870-021-02971-1 |
work_keys_str_mv |
AT wenshuangxie simultaneousgeneexpressionandmultigenesilencinginzeamaysusingmaizedwarfmosaicvirus AT deemariemarty simultaneousgeneexpressionandmultigenesilencinginzeamaysusingmaizedwarfmosaicvirus AT junhuanxu simultaneousgeneexpressionandmultigenesilencinginzeamaysusingmaizedwarfmosaicvirus AT nitikakhatri simultaneousgeneexpressionandmultigenesilencinginzeamaysusingmaizedwarfmosaicvirus AT kristenwillie simultaneousgeneexpressionandmultigenesilencinginzeamaysusingmaizedwarfmosaicvirus AT wandersonbuckermoraes simultaneousgeneexpressionandmultigenesilencinginzeamaysusingmaizedwarfmosaicvirus AT lucyrstewart simultaneousgeneexpressionandmultigenesilencinginzeamaysusingmaizedwarfmosaicvirus |
_version_ |
1721454495995527168 |