Drug target discovery by magnetic nanoparticles coupled mass spectrometry
Drug target discovery is the basis of drug screening. It elucidates the cause of disease and the mechanism of drug action, which is the essential of drug innovation. Target discovery performed in biological systems is complicated as proteins are in low abundance and endogenous compounds may interfer...
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2021-02-01
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doaj-6a6210d3472d4af6b018e6d4ee18c4202021-04-02T21:38:13ZengElsevierJournal of Pharmaceutical Analysis2095-17792021-02-01111122127Drug target discovery by magnetic nanoparticles coupled mass spectrometryDandan Xia0Baoling Liu1Xiaowei Xu2Ya Ding3Qiuling Zheng4Department of Pharmaceutical Analysis, School of Pharmacy, China Pharmaceutical University, Nanjing, 210009, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, ChinaDepartment of Pharmaceutical Analysis, School of Pharmacy, China Pharmaceutical University, Nanjing, 210009, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China; Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, 210009, ChinaDepartment of Pharmaceutical Analysis, School of Pharmacy, China Pharmaceutical University, Nanjing, 210009, China; Corresponding author.Department of Pharmaceutical Analysis, School of Pharmacy, China Pharmaceutical University, Nanjing, 210009, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China; Corresponding author. Department of Pharmaceutical Analysis, School of Pharmacy, China Pharmaceutical University, Nanjing, 210009, ChinaDrug target discovery is the basis of drug screening. It elucidates the cause of disease and the mechanism of drug action, which is the essential of drug innovation. Target discovery performed in biological systems is complicated as proteins are in low abundance and endogenous compounds may interfere with drug binding. Therefore, methods to track drug-target interactions in biological matrices are urgently required. In this work, a Fe3O4 nanoparticle-based approach was developed for drug-target screening in biofluids. A known ligand-protein complex was selected as a principle-to-proof example to validate the feasibility. After incubation in cell lysates, ligand-modified Fe3O4 nanoparticles bound to the target protein and formed complexes that were separated from the lysates by a magnet for further analysis. The large surface-to-volume ratio of the nanoparticles provides more active sites for the modification of chemical drugs. It enhances the opportunity for ligand-protein interactions, which is beneficial for capturing target proteins, especially for those with low abundance. Additionally, a one-step magnetic separation simplifies the pre-processing of ligand-protein complexes, so it effectively reduces the endogenous interference. Therefore, the present nanoparticle-based approach has the potential to be used for drug target screening in biological systems.http://www.sciencedirect.com/science/article/pii/S2095177919307178Drug-target discoveryNanoparticleMass spectrometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dandan Xia Baoling Liu Xiaowei Xu Ya Ding Qiuling Zheng |
spellingShingle |
Dandan Xia Baoling Liu Xiaowei Xu Ya Ding Qiuling Zheng Drug target discovery by magnetic nanoparticles coupled mass spectrometry Journal of Pharmaceutical Analysis Drug-target discovery Nanoparticle Mass spectrometry |
author_facet |
Dandan Xia Baoling Liu Xiaowei Xu Ya Ding Qiuling Zheng |
author_sort |
Dandan Xia |
title |
Drug target discovery by magnetic nanoparticles coupled mass spectrometry |
title_short |
Drug target discovery by magnetic nanoparticles coupled mass spectrometry |
title_full |
Drug target discovery by magnetic nanoparticles coupled mass spectrometry |
title_fullStr |
Drug target discovery by magnetic nanoparticles coupled mass spectrometry |
title_full_unstemmed |
Drug target discovery by magnetic nanoparticles coupled mass spectrometry |
title_sort |
drug target discovery by magnetic nanoparticles coupled mass spectrometry |
publisher |
Elsevier |
series |
Journal of Pharmaceutical Analysis |
issn |
2095-1779 |
publishDate |
2021-02-01 |
description |
Drug target discovery is the basis of drug screening. It elucidates the cause of disease and the mechanism of drug action, which is the essential of drug innovation. Target discovery performed in biological systems is complicated as proteins are in low abundance and endogenous compounds may interfere with drug binding. Therefore, methods to track drug-target interactions in biological matrices are urgently required. In this work, a Fe3O4 nanoparticle-based approach was developed for drug-target screening in biofluids. A known ligand-protein complex was selected as a principle-to-proof example to validate the feasibility. After incubation in cell lysates, ligand-modified Fe3O4 nanoparticles bound to the target protein and formed complexes that were separated from the lysates by a magnet for further analysis. The large surface-to-volume ratio of the nanoparticles provides more active sites for the modification of chemical drugs. It enhances the opportunity for ligand-protein interactions, which is beneficial for capturing target proteins, especially for those with low abundance. Additionally, a one-step magnetic separation simplifies the pre-processing of ligand-protein complexes, so it effectively reduces the endogenous interference. Therefore, the present nanoparticle-based approach has the potential to be used for drug target screening in biological systems. |
topic |
Drug-target discovery Nanoparticle Mass spectrometry |
url |
http://www.sciencedirect.com/science/article/pii/S2095177919307178 |
work_keys_str_mv |
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1721544849845387264 |