Metabolism study of morpholinium 2- ((4- (2-methoxyphenyl)-5-(pyridin-4-yl) -4H-1,2,4-triazole-3-yl)thio) acetate
Aim. The purpose of this study is to reveal the determination of the structure of the main metabolite of morpholinium 2-(4-(2-methoxyphenyl)-5-(pyridin-4-yl)-4H-1,2,4-triazole-3-yl)thio)acetate (an active pharmaceutical ingredient – API) by way of chromatography through mass spectrometry detection,...
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Online Access: | https://doi.org/10.2478/cipms-2020-0014 |
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doaj-6a849acabdaa4752b860dcfc9b26ec552021-09-06T19:22:04ZengSciendoCurrent Issues in Pharmacy and Medical Sciences2084-980X2300-66762020-06-01332727510.2478/cipms-2020-0014cipms-2020-0014Metabolism study of morpholinium 2- ((4- (2-methoxyphenyl)-5-(pyridin-4-yl) -4H-1,2,4-triazole-3-yl)thio) acetateVarynskyi Borys0Kaplaushenko Andriy1State Medical University, Pharmaceutical Faculty, Zaporozhye, UkraineState Medical University, Pharmaceutical Faculty, Zaporozhye, UkraineAim. The purpose of this study is to reveal the determination of the structure of the main metabolite of morpholinium 2-(4-(2-methoxyphenyl)-5-(pyridin-4-yl)-4H-1,2,4-triazole-3-yl)thio)acetate (an active pharmaceutical ingredient – API) by way of chromatography through mass spectrometry detection, utilizing the liquid chromatography system Agilent 1260 Infinity and mass spectrometry detector (single quadrupole detector Agilent 6120). In the work, the graph of the change in the area of the peak of the metabolite from time after the introduction of the API solution was constructed. Moreover, the charges on the atoms of 2-((4-(2-methoxyphenyl)-5-(pyridin-4-yl)-4H-1,2,4-triazol-3-yl)thio) acetate were calculated and the structure of the methyl derivative was proposed. We saw that the methylation of the active substance during metabolism formed a 5-((carboxymethyl)thio)-4-(2-methoxyphenyl)-1-methyl-3-(pyridin-4-yl)-4H-1,2,4-triazol-1-um cation through N-methyltransferase.https://doi.org/10.2478/cipms-2020-0014124-triazolemetabolismliquid chromatographymass spectrometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Varynskyi Borys Kaplaushenko Andriy |
spellingShingle |
Varynskyi Borys Kaplaushenko Andriy Metabolism study of morpholinium 2- ((4- (2-methoxyphenyl)-5-(pyridin-4-yl) -4H-1,2,4-triazole-3-yl)thio) acetate Current Issues in Pharmacy and Medical Sciences 1 2 4-triazole metabolism liquid chromatography mass spectrometry |
author_facet |
Varynskyi Borys Kaplaushenko Andriy |
author_sort |
Varynskyi Borys |
title |
Metabolism study of morpholinium 2- ((4- (2-methoxyphenyl)-5-(pyridin-4-yl) -4H-1,2,4-triazole-3-yl)thio) acetate |
title_short |
Metabolism study of morpholinium 2- ((4- (2-methoxyphenyl)-5-(pyridin-4-yl) -4H-1,2,4-triazole-3-yl)thio) acetate |
title_full |
Metabolism study of morpholinium 2- ((4- (2-methoxyphenyl)-5-(pyridin-4-yl) -4H-1,2,4-triazole-3-yl)thio) acetate |
title_fullStr |
Metabolism study of morpholinium 2- ((4- (2-methoxyphenyl)-5-(pyridin-4-yl) -4H-1,2,4-triazole-3-yl)thio) acetate |
title_full_unstemmed |
Metabolism study of morpholinium 2- ((4- (2-methoxyphenyl)-5-(pyridin-4-yl) -4H-1,2,4-triazole-3-yl)thio) acetate |
title_sort |
metabolism study of morpholinium 2- ((4- (2-methoxyphenyl)-5-(pyridin-4-yl) -4h-1,2,4-triazole-3-yl)thio) acetate |
publisher |
Sciendo |
series |
Current Issues in Pharmacy and Medical Sciences |
issn |
2084-980X 2300-6676 |
publishDate |
2020-06-01 |
description |
Aim. The purpose of this study is to reveal the determination of the structure of the main metabolite of morpholinium 2-(4-(2-methoxyphenyl)-5-(pyridin-4-yl)-4H-1,2,4-triazole-3-yl)thio)acetate (an active pharmaceutical ingredient – API) by way of chromatography through mass spectrometry detection, utilizing the liquid chromatography system Agilent 1260 Infinity and mass spectrometry detector (single quadrupole detector Agilent 6120). In the work, the graph of the change in the area of the peak of the metabolite from time after the introduction of the API solution was constructed. Moreover, the charges on the atoms of 2-((4-(2-methoxyphenyl)-5-(pyridin-4-yl)-4H-1,2,4-triazol-3-yl)thio) acetate were calculated and the structure of the methyl derivative was proposed. We saw that the methylation of the active substance during metabolism formed a 5-((carboxymethyl)thio)-4-(2-methoxyphenyl)-1-methyl-3-(pyridin-4-yl)-4H-1,2,4-triazol-1-um cation through N-methyltransferase. |
topic |
1 2 4-triazole metabolism liquid chromatography mass spectrometry |
url |
https://doi.org/10.2478/cipms-2020-0014 |
work_keys_str_mv |
AT varynskyiborys metabolismstudyofmorpholinium242methoxyphenyl5pyridin4yl4h124triazole3ylthioacetate AT kaplaushenkoandriy metabolismstudyofmorpholinium242methoxyphenyl5pyridin4yl4h124triazole3ylthioacetate |
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1717772793999785984 |