T follicular helper cells are elevated in a rat model of autoimmune myocarditis
Myocarditis is an inflammatory disease of the myocardium that is associated with immune dysfunction. Earlier studies have suggested that T helper 1/2 cell imbalance plays an important role in the development of myocarditis, but the role of T follicular helper (Tfh) cells in the development of autoim...
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doaj-6a950e527dbc403aaa39da92eec24d552020-11-25T03:47:14ZengWileyFEBS Open Bio2211-54632020-07-011071304131510.1002/2211-5463.12894T follicular helper cells are elevated in a rat model of autoimmune myocarditisQi Xue0Yuan Ma1Lihong Wang2Hong Shao3Department of Cardiology People’s Hospital of Hangzhou Medical College Zhejiang Provincial People’s Hospital Hangzhou ChinaDepartment of Cardiology People’s Hospital of Hangzhou Medical College Zhejiang Provincial People’s Hospital Hangzhou ChinaDepartment of Cardiology People’s Hospital of Hangzhou Medical College Zhejiang Provincial People’s Hospital Hangzhou ChinaDepartment of Cardiology People’s Hospital of Hangzhou Medical College Zhejiang Provincial People’s Hospital Hangzhou ChinaMyocarditis is an inflammatory disease of the myocardium that is associated with immune dysfunction. Earlier studies have suggested that T helper 1/2 cell imbalance plays an important role in the development of myocarditis, but the role of T follicular helper (Tfh) cells in the development of autoimmune myocarditis has not previously been reported. Here, we investigated this involvement by using a rat model of experimental autoimmune myocarditis (EAM). Inflammatory cell infiltration, myocardial structure destruction and tissue necrosis were observed in EAM myocardial tissues, and the percentages of CD4+CXCR5+ Tfh cells and CD19+ B cells were both significantly higher in spleen and myocardial tissues of the EAM model as compared with the control group. Furthermore, the expression levels of interleukin‐21, CXCL13 and myosin antibody were significantly higher in the serum of rats with EAM compared with the control group on days 14 and 35 after immunization. Fourteen or 35 days after immunization, the expression levels of interleukin‐21 and CXCL13 were both significantly higher in myocardial tissues of rats with EAM as compared with the control group. Our findings suggest that Tfh cell balance is disrupted during the pathological process of autoimmune myocarditis.https://doi.org/10.1002/2211-5463.12894B cellsCXCL13experimental autoimmune myocarditisinterleukin‐21T follicular helper cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qi Xue Yuan Ma Lihong Wang Hong Shao |
spellingShingle |
Qi Xue Yuan Ma Lihong Wang Hong Shao T follicular helper cells are elevated in a rat model of autoimmune myocarditis FEBS Open Bio B cells CXCL13 experimental autoimmune myocarditis interleukin‐21 T follicular helper cells |
author_facet |
Qi Xue Yuan Ma Lihong Wang Hong Shao |
author_sort |
Qi Xue |
title |
T follicular helper cells are elevated in a rat model of autoimmune myocarditis |
title_short |
T follicular helper cells are elevated in a rat model of autoimmune myocarditis |
title_full |
T follicular helper cells are elevated in a rat model of autoimmune myocarditis |
title_fullStr |
T follicular helper cells are elevated in a rat model of autoimmune myocarditis |
title_full_unstemmed |
T follicular helper cells are elevated in a rat model of autoimmune myocarditis |
title_sort |
t follicular helper cells are elevated in a rat model of autoimmune myocarditis |
publisher |
Wiley |
series |
FEBS Open Bio |
issn |
2211-5463 |
publishDate |
2020-07-01 |
description |
Myocarditis is an inflammatory disease of the myocardium that is associated with immune dysfunction. Earlier studies have suggested that T helper 1/2 cell imbalance plays an important role in the development of myocarditis, but the role of T follicular helper (Tfh) cells in the development of autoimmune myocarditis has not previously been reported. Here, we investigated this involvement by using a rat model of experimental autoimmune myocarditis (EAM). Inflammatory cell infiltration, myocardial structure destruction and tissue necrosis were observed in EAM myocardial tissues, and the percentages of CD4+CXCR5+ Tfh cells and CD19+ B cells were both significantly higher in spleen and myocardial tissues of the EAM model as compared with the control group. Furthermore, the expression levels of interleukin‐21, CXCL13 and myosin antibody were significantly higher in the serum of rats with EAM compared with the control group on days 14 and 35 after immunization. Fourteen or 35 days after immunization, the expression levels of interleukin‐21 and CXCL13 were both significantly higher in myocardial tissues of rats with EAM as compared with the control group. Our findings suggest that Tfh cell balance is disrupted during the pathological process of autoimmune myocarditis. |
topic |
B cells CXCL13 experimental autoimmune myocarditis interleukin‐21 T follicular helper cells |
url |
https://doi.org/10.1002/2211-5463.12894 |
work_keys_str_mv |
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