Three‐month outcomes of aortic valve reconstruction using collagenous membranes (biosheets) produced by in‐body tissue architecture in a goat model: a preliminary study
Abstract Background Autologous pericardium is widely used as a plastic material in intracardiac structures, in the pulmonary artery, and in aortic valve leaflets. For aortic valve reconstruction (AVRec) using the Ozaki procedure, it has produced excellent clinical results over a 10-year period. In-b...
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doaj-6aa9b17896c1477293368ec61abdca9f2021-04-18T11:27:08ZengBMCBMC Cardiovascular Disorders1471-22612021-04-012111910.1186/s12872-021-01988-6Three‐month outcomes of aortic valve reconstruction using collagenous membranes (biosheets) produced by in‐body tissue architecture in a goat model: a preliminary studyKeitaro Okamoto0Tadashi Umeno1Takashi Shuto2Tomoyuki Wada3Hirofumi Anai4Haruto Nishida5Yasuhide Nakayama6Shinji Miyamoto7Department of Cardiovascular Surgery, Faculty of Medicine, Oita UniversityDepartment of Cardiovascular Surgery, Oita University HospitalDepartment of Cardiovascular Surgery, Oita University HospitalDepartment of Cardiovascular Surgery, Oita University HospitalClinical Engineering, Faculty of Medicine, Oita UniversityDepartment of Pathology, Oita University HospitalBiotube Co., LtdDepartment of Cardiovascular Surgery, Oita University HospitalAbstract Background Autologous pericardium is widely used as a plastic material in intracardiac structures, in the pulmonary artery, and in aortic valve leaflets. For aortic valve reconstruction (AVRec) using the Ozaki procedure, it has produced excellent clinical results over a 10-year period. In-body tissue architecture (iBTA), which is based on the phenomenon of tissue encapsulation of foreign materials, can be used to prepare autologous prosthetic tissues. In this preliminary study, we examined whether biosheets can be used as valve leaflet material for glutaraldehyde-free AVRec by subchronic implantation experiments in goats and evaluated its performance compared with glutaraldehyde-treated autologous pericardium for AVRec. Methods Biosheets were prepared by embedding molds for two months into the dorsal subcutaneous spaces of goats. Autogenic biosheets (n = 4) cut into the shape of the valve were then implanted to the aortic valve annulus of four goats for three months without glutaraldehyde treatment. Autologous pericardium (n = 4) was used in four goats as a control. Valve function was observed using echocardiography. Results All goats survived the three-month study period. With biosheets, the leaflet surfaces were very smooth and, on histology, partially covered with a thin neointima (including endothelial cells). Biosheets were more thoroughly assimilated into the aortic root compared with autologous pericardium. Conclusions For the first time, biosheets were used for large animal AVRec. Biosheets could function as leaflets in the aortic position and may have the ability to assimilate into native valves.https://doi.org/10.1186/s12872-021-01988-6Aortic rootValve repairTissue EngineeringValve replacement |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Keitaro Okamoto Tadashi Umeno Takashi Shuto Tomoyuki Wada Hirofumi Anai Haruto Nishida Yasuhide Nakayama Shinji Miyamoto |
spellingShingle |
Keitaro Okamoto Tadashi Umeno Takashi Shuto Tomoyuki Wada Hirofumi Anai Haruto Nishida Yasuhide Nakayama Shinji Miyamoto Three‐month outcomes of aortic valve reconstruction using collagenous membranes (biosheets) produced by in‐body tissue architecture in a goat model: a preliminary study BMC Cardiovascular Disorders Aortic root Valve repair Tissue Engineering Valve replacement |
author_facet |
Keitaro Okamoto Tadashi Umeno Takashi Shuto Tomoyuki Wada Hirofumi Anai Haruto Nishida Yasuhide Nakayama Shinji Miyamoto |
author_sort |
Keitaro Okamoto |
title |
Three‐month outcomes of aortic valve reconstruction using collagenous membranes (biosheets) produced by in‐body tissue architecture in a goat model: a preliminary study |
title_short |
Three‐month outcomes of aortic valve reconstruction using collagenous membranes (biosheets) produced by in‐body tissue architecture in a goat model: a preliminary study |
title_full |
Three‐month outcomes of aortic valve reconstruction using collagenous membranes (biosheets) produced by in‐body tissue architecture in a goat model: a preliminary study |
title_fullStr |
Three‐month outcomes of aortic valve reconstruction using collagenous membranes (biosheets) produced by in‐body tissue architecture in a goat model: a preliminary study |
title_full_unstemmed |
Three‐month outcomes of aortic valve reconstruction using collagenous membranes (biosheets) produced by in‐body tissue architecture in a goat model: a preliminary study |
title_sort |
three‐month outcomes of aortic valve reconstruction using collagenous membranes (biosheets) produced by in‐body tissue architecture in a goat model: a preliminary study |
publisher |
BMC |
series |
BMC Cardiovascular Disorders |
issn |
1471-2261 |
publishDate |
2021-04-01 |
description |
Abstract Background Autologous pericardium is widely used as a plastic material in intracardiac structures, in the pulmonary artery, and in aortic valve leaflets. For aortic valve reconstruction (AVRec) using the Ozaki procedure, it has produced excellent clinical results over a 10-year period. In-body tissue architecture (iBTA), which is based on the phenomenon of tissue encapsulation of foreign materials, can be used to prepare autologous prosthetic tissues. In this preliminary study, we examined whether biosheets can be used as valve leaflet material for glutaraldehyde-free AVRec by subchronic implantation experiments in goats and evaluated its performance compared with glutaraldehyde-treated autologous pericardium for AVRec. Methods Biosheets were prepared by embedding molds for two months into the dorsal subcutaneous spaces of goats. Autogenic biosheets (n = 4) cut into the shape of the valve were then implanted to the aortic valve annulus of four goats for three months without glutaraldehyde treatment. Autologous pericardium (n = 4) was used in four goats as a control. Valve function was observed using echocardiography. Results All goats survived the three-month study period. With biosheets, the leaflet surfaces were very smooth and, on histology, partially covered with a thin neointima (including endothelial cells). Biosheets were more thoroughly assimilated into the aortic root compared with autologous pericardium. Conclusions For the first time, biosheets were used for large animal AVRec. Biosheets could function as leaflets in the aortic position and may have the ability to assimilate into native valves. |
topic |
Aortic root Valve repair Tissue Engineering Valve replacement |
url |
https://doi.org/10.1186/s12872-021-01988-6 |
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