Extracellular detection of neuronal coupling

Abstract We developed a method to non-invasively detect synaptic relationships among neurons from in vitro networks. Our method uses microelectrode arrays on which neurons are cultured and from which propagation of extracellular action potentials (eAPs) in single axons are recorded at multiple elect...

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Main Authors:	Elmer Guzman, Zhuowei Cheng, Paul K. Hansma, Kenneth R. Tovar, Linda R. Petzold, Kenneth S. Kosik
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-07-01
Series:	Scientific Reports
Online Access:	https://doi.org/10.1038/s41598-021-94282-6

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spelling	doaj-6ac560e02a154a589e0c0676a99ca60d2021-07-25T11:27:20ZengNature Publishing GroupScientific Reports2045-23222021-07-0111111110.1038/s41598-021-94282-6Extracellular detection of neuronal couplingElmer Guzman0Zhuowei Cheng1Paul K. Hansma2Kenneth R. Tovar3Linda R. Petzold4Kenneth S. Kosik5Department of Molecular, Cellular and Developmental Biology, University of California, Santa BarbaraDepartment of Computer Science, University of California, Santa BarbaraDepartment of Physics, University of California, Santa BarbaraNeuroscience Research Institute, University of California, Santa BarbaraDepartment of Computer Science, University of California, Santa BarbaraDepartment of Molecular, Cellular and Developmental Biology, University of California, Santa BarbaraAbstract We developed a method to non-invasively detect synaptic relationships among neurons from in vitro networks. Our method uses microelectrode arrays on which neurons are cultured and from which propagation of extracellular action potentials (eAPs) in single axons are recorded at multiple electrodes. Detecting eAP propagation bypasses ambiguity introduced by spike sorting. Our methods identify short latency spiking relationships between neurons with properties expected of synaptically coupled neurons, namely they were recapitulated by direct stimulation and were sensitive to changing the number of active synaptic sites. Our methods enabled us to assemble a functional subset of neuronal connectivity in our cultures.https://doi.org/10.1038/s41598-021-94282-6
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Elmer Guzman Zhuowei Cheng Paul K. Hansma Kenneth R. Tovar Linda R. Petzold Kenneth S. Kosik
spellingShingle	Elmer Guzman Zhuowei Cheng Paul K. Hansma Kenneth R. Tovar Linda R. Petzold Kenneth S. Kosik Extracellular detection of neuronal coupling Scientific Reports
author_facet	Elmer Guzman Zhuowei Cheng Paul K. Hansma Kenneth R. Tovar Linda R. Petzold Kenneth S. Kosik
author_sort	Elmer Guzman
title	Extracellular detection of neuronal coupling
title_short	Extracellular detection of neuronal coupling
title_full	Extracellular detection of neuronal coupling
title_fullStr	Extracellular detection of neuronal coupling
title_full_unstemmed	Extracellular detection of neuronal coupling
title_sort	extracellular detection of neuronal coupling
publisher	Nature Publishing Group
series	Scientific Reports
issn	2045-2322
publishDate	2021-07-01
description	Abstract We developed a method to non-invasively detect synaptic relationships among neurons from in vitro networks. Our method uses microelectrode arrays on which neurons are cultured and from which propagation of extracellular action potentials (eAPs) in single axons are recorded at multiple electrodes. Detecting eAP propagation bypasses ambiguity introduced by spike sorting. Our methods identify short latency spiking relationships between neurons with properties expected of synaptically coupled neurons, namely they were recapitulated by direct stimulation and were sensitive to changing the number of active synaptic sites. Our methods enabled us to assemble a functional subset of neuronal connectivity in our cultures.
url	https://doi.org/10.1038/s41598-021-94282-6
work_keys_str_mv	AT elmerguzman extracellulardetectionofneuronalcoupling AT zhuoweicheng extracellulardetectionofneuronalcoupling AT paulkhansma extracellulardetectionofneuronalcoupling AT kennethrtovar extracellulardetectionofneuronalcoupling AT lindarpetzold extracellulardetectionofneuronalcoupling AT kennethskosik extracellulardetectionofneuronalcoupling
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Extracellular detection of neuronal coupling

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