Developing Two Rapid Protein Extraction Methods Using Focused-Ultrasonication and Zirconia-Silica Beads for Filamentous Fungi Identification by MALDI-TOF MS

Developing Two Rapid Protein Extraction Methods Using Focused-Ultrasonication and Zirconia-Silica Beads for Filamentous Fungi Identification by MALDI-TOF MS

Filamentous fungi identification by Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been challenging due to the lack of simple and rapid protein extraction methods and insufficient species coverage in the database. In this study, we created two rapid p...

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Bibliographic Details
Main Authors: Ya-Ting Ning, Wen-Hang Yang, Wei Zhang, Meng Xiao, Yao Wang, Jing-Jia Zhang, Ge Zhang, Si-Meng Duan, Ai-Ying Dong, Da-Wen Guo, Gui-Ling Zou, Hai-Nan Wen, Yan-Yan Guo, Li-Ping Chen, Miao Chai, Jing-Dong He, Qiong Duan, Li-Xia Zhang, Li Zhang, Ying-Chun Xu
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-07-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
filamentous fungi
MALDI-TOF MS
protein extraction
sample processing
zirconia-silica beads
focused-ultrasonication
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2021.687240/full

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