Regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil[S]
Omega-3-PUFAs, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), are associated with prevention of various aspects of metabolic syndrome. In the present studies, the effects of oil rich in EPA on gene expression and activation of nuclear receptors was examined and compared with other ω3-P...
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doaj-6acfbf5d933a40049b081db6813884fa2021-04-28T06:05:32ZengElsevierJournal of Lipid Research0022-22752012-08-0153816791689Regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil[S]Peter J. Gillies0Sujata K. Bhatia1Leigh A Belcher2Daniel B. Hannon3Jerry T. Thompson4John P. Vanden Heuvel5Central Research and Development, DuPont Experimental Station, E328/140B, Wilmington, DE 19880Central Research and Development, DuPont Experimental Station, E328/140B, Wilmington, DE 19880DuPont Industrial Biosciences, Experimental Station E356, Wilmington, DE 19880Department of Veterinary and Biomedical Sciences and Center for Molecular Toxicology and Carcinogenesis, Pennsylvania State University, University Park, PA 16802; andDepartment of Veterinary and Biomedical Sciences and Center for Molecular Toxicology and Carcinogenesis, Pennsylvania State University, University Park, PA 16802; andTo whom correspondence should be addressed. e-mail: jpv2@psu.edu.; Department of Veterinary and Biomedical Sciences and Center for Molecular Toxicology and Carcinogenesis, Pennsylvania State University, University Park, PA 16802; and; INDIGO Biosciences, Inc., State College, PA 16801; To whom correspondence should be addressed. e-mail: jpv2@psu.edu.Omega-3-PUFAs, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), are associated with prevention of various aspects of metabolic syndrome. In the present studies, the effects of oil rich in EPA on gene expression and activation of nuclear receptors was examined and compared with other ω3-PUFAs. The EPA-rich oil (EO) altered the expression of FA metabolism genes in THP-1 cells, including stearoyl CoA desaturase (SCD) and FA desaturase-1 and -2 (FASDS1 and -2). Other ω3-PUFAs resulted in a similar gene expression response for a subset of genes involved in lipid metabolism and inflammation. In reporter assays, EO activated human peroxisome proliferator-activated receptor α (PPARα) and PPARβ/γ with minimal effects on PPARγ, liver X receptor, retinoid X receptor, farnesoid X receptor, and retinoid acid receptor γ (RARγ); these effects were similar to that observed for purified EPA. When serum from a 6 week clinical intervention with dietary supplements containing olive oil (control), DHA, or two levels of EPA were applied to THP-1 cells, the expression of SCD and FADS2 decreased in the cells treated with serum from the ω3-PUFA-supplemented individuals. Taken together, these studies indicate regulation of gene expression by EO that is consistent with treating aspects of dyslipidemia and inflammation.http://www.sciencedirect.com/science/article/pii/S0022227520418723omega-3 polyunsaturated fatty acidsteroyl coenzyme A desaturasefatty acid desaturaseperoxisome proliferator-activated receptornuclear receptor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Peter J. Gillies Sujata K. Bhatia Leigh A Belcher Daniel B. Hannon Jerry T. Thompson John P. Vanden Heuvel |
spellingShingle |
Peter J. Gillies Sujata K. Bhatia Leigh A Belcher Daniel B. Hannon Jerry T. Thompson John P. Vanden Heuvel Regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil[S] Journal of Lipid Research omega-3 polyunsaturated fatty acid steroyl coenzyme A desaturase fatty acid desaturase peroxisome proliferator-activated receptor nuclear receptor |
author_facet |
Peter J. Gillies Sujata K. Bhatia Leigh A Belcher Daniel B. Hannon Jerry T. Thompson John P. Vanden Heuvel |
author_sort |
Peter J. Gillies |
title |
Regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil[S] |
title_short |
Regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil[S] |
title_full |
Regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil[S] |
title_fullStr |
Regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil[S] |
title_full_unstemmed |
Regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil[S] |
title_sort |
regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil[s] |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2012-08-01 |
description |
Omega-3-PUFAs, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), are associated with prevention of various aspects of metabolic syndrome. In the present studies, the effects of oil rich in EPA on gene expression and activation of nuclear receptors was examined and compared with other ω3-PUFAs. The EPA-rich oil (EO) altered the expression of FA metabolism genes in THP-1 cells, including stearoyl CoA desaturase (SCD) and FA desaturase-1 and -2 (FASDS1 and -2). Other ω3-PUFAs resulted in a similar gene expression response for a subset of genes involved in lipid metabolism and inflammation. In reporter assays, EO activated human peroxisome proliferator-activated receptor α (PPARα) and PPARβ/γ with minimal effects on PPARγ, liver X receptor, retinoid X receptor, farnesoid X receptor, and retinoid acid receptor γ (RARγ); these effects were similar to that observed for purified EPA. When serum from a 6 week clinical intervention with dietary supplements containing olive oil (control), DHA, or two levels of EPA were applied to THP-1 cells, the expression of SCD and FADS2 decreased in the cells treated with serum from the ω3-PUFA-supplemented individuals. Taken together, these studies indicate regulation of gene expression by EO that is consistent with treating aspects of dyslipidemia and inflammation. |
topic |
omega-3 polyunsaturated fatty acid steroyl coenzyme A desaturase fatty acid desaturase peroxisome proliferator-activated receptor nuclear receptor |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520418723 |
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