Interleukin 8 (-251 T>A) polymorphism in children and teenagers infected with Helicobacter pylori
Abstract Background Helicobacter pylori (H. pylori) is a gram-negative bacterium that colonizes the human stomach and causes a variety of gastric diseases. This study evaluated the correlations between the -251 (T>A) (rs4073) polymorphism of interleukin-8 (IL-8), the etiology of gastric disease,...
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doaj-6ad472441ae6462480d6214efef00db32020-11-24T21:37:06ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992017-04-012311310.1186/s40409-017-0113-zInterleukin 8 (-251 T>A) polymorphism in children and teenagers infected with Helicobacter pyloriMarina Saes0Roger Willian de Labio1Lucas Trevizani Rasmussen2Spencer Luiz Marques Payão3Faculdade de Medicina de Marília (FAMEMA)Faculdade de Medicina de Marília (FAMEMA)Universidade do Sagrado CoraçãoFaculdade de Medicina de Marília (FAMEMA)Abstract Background Helicobacter pylori (H. pylori) is a gram-negative bacterium that colonizes the human stomach and causes a variety of gastric diseases. This study evaluated the correlations between the -251 (T>A) (rs4073) polymorphism of interleukin-8 (IL-8), the etiology of gastric disease, and H. pylori infection in pediatric and adolescent patients. Methods DNA samples were obtained from 285 gastric biopsies from pediatric patients. H. pylori was detected by PCR, whereas PCR-RFLP was used to characterize the -251 (T>A) polymorphism of IL-8. Results The histological analysis revealed the presence of gastritis in 158 patients (55.44%). H. pylori was found in 71 samples (24.9%). The -251 (T>A) polymorphism revealed that 58 (29.47%) samples were TT, 143 (50.18%) samples were TA, and 84 (20.35%) samples were AA. Conclusions Our findings suggest that IL8-251 A allele may be an important risk factor for the development of gastric disease when associated with H. pylori infection.http://link.springer.com/article/10.1186/s40409-017-0113-zHelicobacter pyloriInterleukin 8Polymorphism-251 (T>A)Gastric disease |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marina Saes Roger Willian de Labio Lucas Trevizani Rasmussen Spencer Luiz Marques Payão |
spellingShingle |
Marina Saes Roger Willian de Labio Lucas Trevizani Rasmussen Spencer Luiz Marques Payão Interleukin 8 (-251 T>A) polymorphism in children and teenagers infected with Helicobacter pylori Journal of Venomous Animals and Toxins including Tropical Diseases Helicobacter pylori Interleukin 8 Polymorphism -251 (T>A) Gastric disease |
author_facet |
Marina Saes Roger Willian de Labio Lucas Trevizani Rasmussen Spencer Luiz Marques Payão |
author_sort |
Marina Saes |
title |
Interleukin 8 (-251 T>A) polymorphism in children and teenagers infected with Helicobacter pylori |
title_short |
Interleukin 8 (-251 T>A) polymorphism in children and teenagers infected with Helicobacter pylori |
title_full |
Interleukin 8 (-251 T>A) polymorphism in children and teenagers infected with Helicobacter pylori |
title_fullStr |
Interleukin 8 (-251 T>A) polymorphism in children and teenagers infected with Helicobacter pylori |
title_full_unstemmed |
Interleukin 8 (-251 T>A) polymorphism in children and teenagers infected with Helicobacter pylori |
title_sort |
interleukin 8 (-251 t>a) polymorphism in children and teenagers infected with helicobacter pylori |
publisher |
SciELO |
series |
Journal of Venomous Animals and Toxins including Tropical Diseases |
issn |
1678-9199 |
publishDate |
2017-04-01 |
description |
Abstract Background Helicobacter pylori (H. pylori) is a gram-negative bacterium that colonizes the human stomach and causes a variety of gastric diseases. This study evaluated the correlations between the -251 (T>A) (rs4073) polymorphism of interleukin-8 (IL-8), the etiology of gastric disease, and H. pylori infection in pediatric and adolescent patients. Methods DNA samples were obtained from 285 gastric biopsies from pediatric patients. H. pylori was detected by PCR, whereas PCR-RFLP was used to characterize the -251 (T>A) polymorphism of IL-8. Results The histological analysis revealed the presence of gastritis in 158 patients (55.44%). H. pylori was found in 71 samples (24.9%). The -251 (T>A) polymorphism revealed that 58 (29.47%) samples were TT, 143 (50.18%) samples were TA, and 84 (20.35%) samples were AA. Conclusions Our findings suggest that IL8-251 A allele may be an important risk factor for the development of gastric disease when associated with H. pylori infection. |
topic |
Helicobacter pylori Interleukin 8 Polymorphism -251 (T>A) Gastric disease |
url |
http://link.springer.com/article/10.1186/s40409-017-0113-z |
work_keys_str_mv |
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