Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (<i>Sigmodon hispidus</i>)
Most mammalian ovarian follicles contain only a single oocyte having a single nucleus. However, two or more oocytes and nuclei are observed within one follicle and one oocyte, respectively, in several species, including cotton rat (CR, <i>Sigmodon hispidus</i>). The present study compare...
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doaj-6af3580966e6448383f227e875afb7862021-07-01T00:03:51ZengMDPI AGAnimals2076-26152021-06-01111768176810.3390/ani11061768Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (<i>Sigmodon hispidus</i>)Md Rashedul Islam0Osamu Ichii1Teppei Nakamura2Takao Irie3Akio Shinohara4Md Abdul Masum5Yuki Otani6Takashi Namba7Tsolmon Chuluunbaatar8Yaser Hosny Ali Elewa9Yasuhiro Kon10Laboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, JapanLaboratory of Veterinary Parasitology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, JapanFrontier Science Research Center, University of Miyazaki, Miyazaki 889-1692, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, JapanMost mammalian ovarian follicles contain only a single oocyte having a single nucleus. However, two or more oocytes and nuclei are observed within one follicle and one oocyte, respectively, in several species, including cotton rat (CR, <i>Sigmodon hispidus</i>). The present study compared ovarian histology, focusing on folliculogenesis, between two inbred CR strains, HIS/Hiph and HIS/Mz. At 4 weeks of age, ovarian sections from both the strains were analyzed histologically. Multi-oocyte follicles (MOFs) and double-nucleated oocytes (DNOs) were observed in all stages of developing follicles in HIS/Hiph, whereas HIS/Mz had MOFs up to secondary stages and lacked DNOs. The estimated total follicles in HIS/Mz were almost half that of HIS/Hiph, but interstitial cells were well developed in HIS/Mz. Furthermore, immunostaining revealed no clear strain differences in the appearance of oocytes positive for Ki67, PCNA, and p63 in MOF or DNOs; no cell death was observed in these oocytes. Ultrastructural analysis revealed more abundant mitochondrial clouds in oocytes of HIS/Hiph than HIS/Mz. Thus, we clarified the strain differences in the CR ovary. These findings indicate that early events during folliculogenesis affect the unique ovarian phenotypes found in CRs, including MOFs or DNOs, and their strain differences.https://www.mdpi.com/2076-2615/11/6/1768cotton ratmulti-oocyte folliclesdouble nucleated oocytesproliferative cellsmitochondrial clouds |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Md Rashedul Islam Osamu Ichii Teppei Nakamura Takao Irie Akio Shinohara Md Abdul Masum Yuki Otani Takashi Namba Tsolmon Chuluunbaatar Yaser Hosny Ali Elewa Yasuhiro Kon |
spellingShingle |
Md Rashedul Islam Osamu Ichii Teppei Nakamura Takao Irie Akio Shinohara Md Abdul Masum Yuki Otani Takashi Namba Tsolmon Chuluunbaatar Yaser Hosny Ali Elewa Yasuhiro Kon Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (<i>Sigmodon hispidus</i>) Animals cotton rat multi-oocyte follicles double nucleated oocytes proliferative cells mitochondrial clouds |
author_facet |
Md Rashedul Islam Osamu Ichii Teppei Nakamura Takao Irie Akio Shinohara Md Abdul Masum Yuki Otani Takashi Namba Tsolmon Chuluunbaatar Yaser Hosny Ali Elewa Yasuhiro Kon |
author_sort |
Md Rashedul Islam |
title |
Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (<i>Sigmodon hispidus</i>) |
title_short |
Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (<i>Sigmodon hispidus</i>) |
title_full |
Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (<i>Sigmodon hispidus</i>) |
title_fullStr |
Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (<i>Sigmodon hispidus</i>) |
title_full_unstemmed |
Comparison of Ovarian Morphology and Follicular Disturbances between Two Inbred Strains of Cotton Rats (<i>Sigmodon hispidus</i>) |
title_sort |
comparison of ovarian morphology and follicular disturbances between two inbred strains of cotton rats (<i>sigmodon hispidus</i>) |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2021-06-01 |
description |
Most mammalian ovarian follicles contain only a single oocyte having a single nucleus. However, two or more oocytes and nuclei are observed within one follicle and one oocyte, respectively, in several species, including cotton rat (CR, <i>Sigmodon hispidus</i>). The present study compared ovarian histology, focusing on folliculogenesis, between two inbred CR strains, HIS/Hiph and HIS/Mz. At 4 weeks of age, ovarian sections from both the strains were analyzed histologically. Multi-oocyte follicles (MOFs) and double-nucleated oocytes (DNOs) were observed in all stages of developing follicles in HIS/Hiph, whereas HIS/Mz had MOFs up to secondary stages and lacked DNOs. The estimated total follicles in HIS/Mz were almost half that of HIS/Hiph, but interstitial cells were well developed in HIS/Mz. Furthermore, immunostaining revealed no clear strain differences in the appearance of oocytes positive for Ki67, PCNA, and p63 in MOF or DNOs; no cell death was observed in these oocytes. Ultrastructural analysis revealed more abundant mitochondrial clouds in oocytes of HIS/Hiph than HIS/Mz. Thus, we clarified the strain differences in the CR ovary. These findings indicate that early events during folliculogenesis affect the unique ovarian phenotypes found in CRs, including MOFs or DNOs, and their strain differences. |
topic |
cotton rat multi-oocyte follicles double nucleated oocytes proliferative cells mitochondrial clouds |
url |
https://www.mdpi.com/2076-2615/11/6/1768 |
work_keys_str_mv |
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