Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs
<p>Abstract</p> <p>Background</p> <p>Avian leukosis virus (ALV) is a major infectious disease that impacts the poultry industry worldwide. Despite intensive efforts, no effective vaccine has been developed against ALV because of mutations that lead to resistant forms. T...
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doaj-6af3854c90214c95a6484ce7765879ae2020-11-24T22:16:22ZengBMCVirology Journal1743-422X2011-12-018155610.1186/1743-422X-8-556Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAsMeng Qing-WenZhang Zai-PingWang WeiTian JinXiao Zhi-Guang<p>Abstract</p> <p>Background</p> <p>Avian leukosis virus (ALV) is a major infectious disease that impacts the poultry industry worldwide. Despite intensive efforts, no effective vaccine has been developed against ALV because of mutations that lead to resistant forms. Therefore, there is a dire need to develop antiviral agents for the treatment of ALV infections and RNA interference (RNAi) is considered an effective antiviral strategy.</p> <p>Results</p> <p>In this study, the avian leukosis virus subgroup J (ALV-J) proviral genome, including the <it>gag </it>genes, were treated as targets for RNAi. Four pairs of miRNA sequences were designed and synthesized that targeted different regions of the <it>gag </it>gene. The screened target (i.e., the <it>gag </it>genes) was shown to effectively suppress the replication of ALV-J by 19.0-77.3%. To avoid the generation of escape variants during virus infection, expression vectors of multi-target miRNAs were constructed using the multi-target serial strategy (against different regions of the <it>gag</it>, <it>pol</it>, and <it>env </it>genes). Multi-target miRNAs were shown to play a synergistic role in the inhibition of ALV-J replication, with an inhibition efficiency of viral replication ranging from 85.0-91.2%.</p> <p>Conclusion</p> <p>The strategy of multi-target miRNAs might be an effective method for inhibiting ALV replication and the acquisition of resistant mutations.</p> http://www.virologyj.com/content/8/1/556ALVmiRNAInhibitionGagMulti-target series |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Meng Qing-Wen Zhang Zai-Ping Wang Wei Tian Jin Xiao Zhi-Guang |
spellingShingle |
Meng Qing-Wen Zhang Zai-Ping Wang Wei Tian Jin Xiao Zhi-Guang Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs Virology Journal ALV miRNA Inhibition Gag Multi-target series |
author_facet |
Meng Qing-Wen Zhang Zai-Ping Wang Wei Tian Jin Xiao Zhi-Guang |
author_sort |
Meng Qing-Wen |
title |
Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs |
title_short |
Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs |
title_full |
Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs |
title_fullStr |
Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs |
title_full_unstemmed |
Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs |
title_sort |
enhanced inhibition of avian leukosis virus subgroup j replication by multi-target mirnas |
publisher |
BMC |
series |
Virology Journal |
issn |
1743-422X |
publishDate |
2011-12-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Avian leukosis virus (ALV) is a major infectious disease that impacts the poultry industry worldwide. Despite intensive efforts, no effective vaccine has been developed against ALV because of mutations that lead to resistant forms. Therefore, there is a dire need to develop antiviral agents for the treatment of ALV infections and RNA interference (RNAi) is considered an effective antiviral strategy.</p> <p>Results</p> <p>In this study, the avian leukosis virus subgroup J (ALV-J) proviral genome, including the <it>gag </it>genes, were treated as targets for RNAi. Four pairs of miRNA sequences were designed and synthesized that targeted different regions of the <it>gag </it>gene. The screened target (i.e., the <it>gag </it>genes) was shown to effectively suppress the replication of ALV-J by 19.0-77.3%. To avoid the generation of escape variants during virus infection, expression vectors of multi-target miRNAs were constructed using the multi-target serial strategy (against different regions of the <it>gag</it>, <it>pol</it>, and <it>env </it>genes). Multi-target miRNAs were shown to play a synergistic role in the inhibition of ALV-J replication, with an inhibition efficiency of viral replication ranging from 85.0-91.2%.</p> <p>Conclusion</p> <p>The strategy of multi-target miRNAs might be an effective method for inhibiting ALV replication and the acquisition of resistant mutations.</p> |
topic |
ALV miRNA Inhibition Gag Multi-target series |
url |
http://www.virologyj.com/content/8/1/556 |
work_keys_str_mv |
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1725790336219873280 |