Comparación de dos protocolos de extracción de ADN de Trypanosoma cruzi cultivados en medio axénico

Comparación de dos protocolos de extracción de ADN de Trypanosoma cruzi cultivados en medio axénico

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Objetivos. Comparar dos protocolos de extracción de ADN de Trypanosoma cruzi para su uso en la amplificación de ADN de minicírculos de kinetoplasto (ADNk) mediante la técnica de Reacción en Cadena de Polimerasa (PCR). Materiales y métodos. Se cultivaron epimastigotas de T. cruzi en condiciones exéni...

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Bibliographic Details
Main Authors:	Mariela López, María G Rivera, Mercedes Viettri, María Lares, Antonio Morocoima, Leidi Herrera, Elizabeth Ferrer
Format:	Article
Language:	Spanish
Published:	Instituto Nacional de Salud
Series:	Revista Peruana de Medicina Experimental y Salud Pública
Subjects:	Trypanosoma cruzi Chagas disease DNA Diagnosis Polymerase chain reaction
Online Access:	http://www.scielo.org.pe/scielo.php?script=sci_arttext&pid=S1726-46342014000200005&lng=en&tlng=en

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