Establishing Cell Lines from Fresh or Cryopreserved Tissue from the Great Crested Newt (<i>Triturus cristatus</i>):A Preliminary Protocol

Establishing Cell Lines from Fresh or Cryopreserved Tissue from the Great Crested Newt (<i>Triturus cristatus</i>):A Preliminary Protocol

This study describes a successful protocol for establishing cell lines from the threatened <i>Triturus cristatus</i> in terms of collection, preparing, establishing, cryopreserving, thawing and quality checking. Different parameters such as media, media change, fresh vs. cryopreserved ti...

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Bibliographic Details
Main Authors:	Julie Strand, Henrik Callesen, Cino Pertoldi, Stig Purup
Format:	Article
Language:	English
Published:	MDPI AG 2021-02-01
Series:	Animals
Subjects:	cell culture morphology culture conditions xCELLigence<sup>®</sup>, real-time cell analysis amphibian cryopreservation
Online Access:	https://www.mdpi.com/2076-2615/11/2/367

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