Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis
Abstract Cytomegalovirus (CMV) reactivation in the colon is common in patients with severe ulcerative colitis (UC). Ganciclovir (GCV) resistance conferring CMV UL97 gene mutations have been reported in recent years. However, the prevalence of UL97 gene mutations in GCV-naive CMV infection in the col...
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doaj-6b4d912ca3a04a1d8a4240916d4ef4692021-07-04T11:28:12ZengNature Publishing GroupScientific Reports2045-23222021-07-011111810.1038/s41598-021-93168-xPrevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitisSatoshi Tamura0Satoshi Osawa1Natsuki Ishida2Takahiro Miyazu3Shinya Tani4Mihoko Yamade5Moriya Iwaizumi6Yasushi Hamaya7Isao Kosugi8Takahisa Furuta9Ken Sugimoto10Department of Endoscopic and Photodynamic Medicine, Hamamatsu University School of MedicineDepartment of Endoscopic and Photodynamic Medicine, Hamamatsu University School of MedicineFirst Department of Medicine, Hamamatsu University School of MedicineFirst Department of Medicine, Hamamatsu University School of MedicineFirst Department of Medicine, Hamamatsu University School of MedicineFirst Department of Medicine, Hamamatsu University School of MedicineDepartment of Laboratory Medicine, Hamamatsu University School of MedicineFirst Department of Medicine, Hamamatsu University School of MedicineDepartment of Regenerative and Infectious Pathology, Hamamatsu University School of MedicineCenter for Clinical Research, Hamamatsu University School of MedicineFirst Department of Medicine, Hamamatsu University School of MedicineAbstract Cytomegalovirus (CMV) reactivation in the colon is common in patients with severe ulcerative colitis (UC). Ganciclovir (GCV) resistance conferring CMV UL97 gene mutations have been reported in recent years. However, the prevalence of UL97 gene mutations in GCV-naive CMV infection in the colon remains unknown. We investigated the prevalence of CMV UL97 gene mutations in patients with colonic CMV infection associated with or without UC. Twenty-two GCV-naive patients with colonic CMV infection, 15 with UC and 7 with other diseases, were enrolled. Frozen biopsy samples or formalin-fixed paraffin-embedded samples were used for nested polymerase chain reaction (PCR) amplification of the UL97 gene. Sanger DNA sequencing was performed. In comparison with AD169 reference strain, natural polymorphisms were frequently detected in codons N68D (100%), I244V (100%), and D605E (86.4%). Seven polymorphisms were detected infrequently (< 10%) outside the kinase domain. However, no known GCV resistance mutations were found. There seemed to be no difference between the ratio of polymorphisms in patients with and without UC. In conclusions, we did not detect UL97 gene mutations associated with GCV resistance in GCV-naive patients with or without UC. Consistent with previous reports, D605E polymorphism may be used as a genetic marker for CMV in East Asian countries.https://doi.org/10.1038/s41598-021-93168-x |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Satoshi Tamura Satoshi Osawa Natsuki Ishida Takahiro Miyazu Shinya Tani Mihoko Yamade Moriya Iwaizumi Yasushi Hamaya Isao Kosugi Takahisa Furuta Ken Sugimoto |
spellingShingle |
Satoshi Tamura Satoshi Osawa Natsuki Ishida Takahiro Miyazu Shinya Tani Mihoko Yamade Moriya Iwaizumi Yasushi Hamaya Isao Kosugi Takahisa Furuta Ken Sugimoto Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis Scientific Reports |
author_facet |
Satoshi Tamura Satoshi Osawa Natsuki Ishida Takahiro Miyazu Shinya Tani Mihoko Yamade Moriya Iwaizumi Yasushi Hamaya Isao Kosugi Takahisa Furuta Ken Sugimoto |
author_sort |
Satoshi Tamura |
title |
Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis |
title_short |
Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis |
title_full |
Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis |
title_fullStr |
Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis |
title_full_unstemmed |
Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis |
title_sort |
prevalence of ul97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-07-01 |
description |
Abstract Cytomegalovirus (CMV) reactivation in the colon is common in patients with severe ulcerative colitis (UC). Ganciclovir (GCV) resistance conferring CMV UL97 gene mutations have been reported in recent years. However, the prevalence of UL97 gene mutations in GCV-naive CMV infection in the colon remains unknown. We investigated the prevalence of CMV UL97 gene mutations in patients with colonic CMV infection associated with or without UC. Twenty-two GCV-naive patients with colonic CMV infection, 15 with UC and 7 with other diseases, were enrolled. Frozen biopsy samples or formalin-fixed paraffin-embedded samples were used for nested polymerase chain reaction (PCR) amplification of the UL97 gene. Sanger DNA sequencing was performed. In comparison with AD169 reference strain, natural polymorphisms were frequently detected in codons N68D (100%), I244V (100%), and D605E (86.4%). Seven polymorphisms were detected infrequently (< 10%) outside the kinase domain. However, no known GCV resistance mutations were found. There seemed to be no difference between the ratio of polymorphisms in patients with and without UC. In conclusions, we did not detect UL97 gene mutations associated with GCV resistance in GCV-naive patients with or without UC. Consistent with previous reports, D605E polymorphism may be used as a genetic marker for CMV in East Asian countries. |
url |
https://doi.org/10.1038/s41598-021-93168-x |
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