Characterization of Cecal Smooth Muscle Contraction in Laying Hens
The ceca play an important role in the physiology of the gastrointestinal tract in chickens. Nevertheless, there is a gap of knowledge regarding the functionality of the ceca in poultry, especially with respect to physiological cecal smooth muscle contraction. The aim of the current study is the ex...
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doaj-6b769531c0da40bb845f8b2452b17cd02021-06-01T01:07:24ZengMDPI AGVeterinary Sciences2306-73812021-05-018919110.3390/vetsci8060091Characterization of Cecal Smooth Muscle Contraction in Laying HensKatrin Röhm0Martin Diener1Korinna Huber2Jana Seifert3Institute of Animal Science, University of Hohenheim, 70593 Stuttgart, GermanyInstitute of Veterinary Physiology and Biochemistry, Justus-Liebig University, 35392 Gießen, GermanyInstitute of Animal Science, University of Hohenheim, 70593 Stuttgart, GermanyInstitute of Animal Science, University of Hohenheim, 70593 Stuttgart, GermanyThe ceca play an important role in the physiology of the gastrointestinal tract in chickens. Nevertheless, there is a gap of knowledge regarding the functionality of the ceca in poultry, especially with respect to physiological cecal smooth muscle contraction. The aim of the current study is the ex vivo characterization of cecal smooth muscle contraction in laying hens. Muscle strips of circular cecal smooth muscle from eleven hens are prepared to investigate their contraction ex vivo. Contraction is detected using an isometric force transducer, determining its frequency, height and intensity. Spontaneous contraction of the chicken cecal smooth muscle and the influence of buffers (calcium-free buffer and potassium-enriched buffer) and drugs (carbachol, nitroprusside, isoprenaline and Verapamil) affecting smooth muscle contraction at different levels are characterized. A decrease in smooth muscle contraction is observed when a calcium-free buffer is used. Carbachol causes an increase in smooth muscle contraction, whereas atropine inhibits contraction. Nitroprusside, isoprenaline and Verapamil result in a depression of smooth muscle contraction. In conclusion, the present results confirm a similar contraction behavior of cecal smooth muscles in laying hens as shown previously in other species.https://www.mdpi.com/2306-7381/8/6/91<i>Gallus gallus</i>laying hensmooth musclecontractionceca |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katrin Röhm Martin Diener Korinna Huber Jana Seifert |
spellingShingle |
Katrin Röhm Martin Diener Korinna Huber Jana Seifert Characterization of Cecal Smooth Muscle Contraction in Laying Hens Veterinary Sciences <i>Gallus gallus</i> laying hen smooth muscle contraction ceca |
author_facet |
Katrin Röhm Martin Diener Korinna Huber Jana Seifert |
author_sort |
Katrin Röhm |
title |
Characterization of Cecal Smooth Muscle Contraction in Laying Hens |
title_short |
Characterization of Cecal Smooth Muscle Contraction in Laying Hens |
title_full |
Characterization of Cecal Smooth Muscle Contraction in Laying Hens |
title_fullStr |
Characterization of Cecal Smooth Muscle Contraction in Laying Hens |
title_full_unstemmed |
Characterization of Cecal Smooth Muscle Contraction in Laying Hens |
title_sort |
characterization of cecal smooth muscle contraction in laying hens |
publisher |
MDPI AG |
series |
Veterinary Sciences |
issn |
2306-7381 |
publishDate |
2021-05-01 |
description |
The ceca play an important role in the physiology of the gastrointestinal tract in chickens. Nevertheless, there is a gap of knowledge regarding the functionality of the ceca in poultry, especially with respect to physiological cecal smooth muscle contraction. The aim of the current study is the ex vivo characterization of cecal smooth muscle contraction in laying hens. Muscle strips of circular cecal smooth muscle from eleven hens are prepared to investigate their contraction ex vivo. Contraction is detected using an isometric force transducer, determining its frequency, height and intensity. Spontaneous contraction of the chicken cecal smooth muscle and the influence of buffers (calcium-free buffer and potassium-enriched buffer) and drugs (carbachol, nitroprusside, isoprenaline and Verapamil) affecting smooth muscle contraction at different levels are characterized. A decrease in smooth muscle contraction is observed when a calcium-free buffer is used. Carbachol causes an increase in smooth muscle contraction, whereas atropine inhibits contraction. Nitroprusside, isoprenaline and Verapamil result in a depression of smooth muscle contraction. In conclusion, the present results confirm a similar contraction behavior of cecal smooth muscles in laying hens as shown previously in other species. |
topic |
<i>Gallus gallus</i> laying hen smooth muscle contraction ceca |
url |
https://www.mdpi.com/2306-7381/8/6/91 |
work_keys_str_mv |
AT katrinrohm characterizationofcecalsmoothmusclecontractioninlayinghens AT martindiener characterizationofcecalsmoothmusclecontractioninlayinghens AT korinnahuber characterizationofcecalsmoothmusclecontractioninlayinghens AT janaseifert characterizationofcecalsmoothmusclecontractioninlayinghens |
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1721413073730797568 |