Trichostrongylus colubriformis: Possible Most Common Cause of Human Infection in Mazandaran Province, North of Iran

• Main Authors: Shirzad Gholami, Farhang Babamahmoodi, Rohallah Abedian, Mehdi Sharif, Abbas Shahbazi, Abdolsattar Pagheh, Mahdi Fakhar
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2015-03-01
• Series: Iranian Journal of Parasitology
• Subjects: Human; ITS2-PCR; Iran; Trichostrongylus axei; Trichostrongylus colubriformis
• Online Access: https://ijpa.tums.ac.ir/index.php/ijpa/article/view/345

  Background: Infection with Trichostrongylus spp. is common among human and herbivorous in most parts of Iran, especially in southern and northern ar-eas. The aim of present study was to identify Trichostrongylus spp. among hu-man population using excreted egg specimens, by the molecular method, in Mazandaran Province, northern Iran. Methods:Overall, 33 positive fecal specimens were randomly sampled and examined. PCR amplification of ITS2-rDNA region was performed on the isolated egg and then a restriction fragment length polymorphism (RFLP) profile was considered to discriminate of Trichostrongylus spp. Results:A total of 33 positive fecal specimens, 29(78.9%), 4(12.1%) were found T. colubriformis and T. axei respectively. Our data appear the molecular evidence of both human T. colubriformis and T. axei infections in North of Iran. Conclusion:T. colubriformis was the probable most common zoonotic species caus-ing human trichostrongylosis infection in the area