A Simple and Reliable Single Tube Septuple PCR Assay for Simultaneous Identification of Seven Meat Species

Multiplex PCR methods have been frequently used for authentication of meat product adulteration. Through screening of new species-specific primers designed based on the mitochondrial DNA sequences, a septuple PCR method is ultimately developed and optimized to simultaneously detect seven species inc...

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Main Authors:	Zhendong Cai, Song Zhou, Qianqian Liu, Hui Ma, Xinyi Yuan, Jiaqi Gao, Jinxuan Cao, Daodong Pan
Format:	Article
Language:	English
Published:	MDPI AG 2021-05-01
Series:	Foods
Subjects:	septuple PCR adulteration meat species mitochondrial genes multiplex PCR
Online Access:	https://www.mdpi.com/2304-8158/10/5/1083

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record_format	Article
spelling	doaj-6bbe33c1edc749f7a3236f02db02ad322021-05-31T23:58:33ZengMDPI AGFoods2304-81582021-05-01101083108310.3390/foods10051083A Simple and Reliable Single Tube Septuple PCR Assay for Simultaneous Identification of Seven Meat SpeciesZhendong Cai0Song Zhou1Qianqian Liu2Hui Ma3Xinyi Yuan4Jiaqi Gao5Jinxuan Cao6Daodong Pan7State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, ChinaInstitute of Environmental Research at Greater Bay Area, Guangzhou University, Guangzhou 510006, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, ChinaMultiplex PCR methods have been frequently used for authentication of meat product adulteration. Through screening of new species-specific primers designed based on the mitochondrial DNA sequences, a septuple PCR method is ultimately developed and optimized to simultaneously detect seven species including turkey (110 bp), goose (194 bp), pig (254 bp), sheep (329 bp), beef (473 bp), chicken (612 bp) and duck (718 bp) in one reaction. The proposed method has been validated to be specific, sensitive, robust and inexpensive. Taken together, the developed septuple PCR assay is reliable and efficient, not only to authenticate animal species in commercial meat products, but also easily feasible in a general laboratory without special infrastructures.https://www.mdpi.com/2304-8158/10/5/1083septuple PCRadulterationmeat speciesmitochondrial genesmultiplex PCR
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Zhendong Cai Song Zhou Qianqian Liu Hui Ma Xinyi Yuan Jiaqi Gao Jinxuan Cao Daodong Pan
spellingShingle	Zhendong Cai Song Zhou Qianqian Liu Hui Ma Xinyi Yuan Jiaqi Gao Jinxuan Cao Daodong Pan A Simple and Reliable Single Tube Septuple PCR Assay for Simultaneous Identification of Seven Meat Species Foods septuple PCR adulteration meat species mitochondrial genes multiplex PCR
author_facet	Zhendong Cai Song Zhou Qianqian Liu Hui Ma Xinyi Yuan Jiaqi Gao Jinxuan Cao Daodong Pan
author_sort	Zhendong Cai
title	A Simple and Reliable Single Tube Septuple PCR Assay for Simultaneous Identification of Seven Meat Species
title_short	A Simple and Reliable Single Tube Septuple PCR Assay for Simultaneous Identification of Seven Meat Species
title_full	A Simple and Reliable Single Tube Septuple PCR Assay for Simultaneous Identification of Seven Meat Species
title_fullStr	A Simple and Reliable Single Tube Septuple PCR Assay for Simultaneous Identification of Seven Meat Species
title_full_unstemmed	A Simple and Reliable Single Tube Septuple PCR Assay for Simultaneous Identification of Seven Meat Species
title_sort	simple and reliable single tube septuple pcr assay for simultaneous identification of seven meat species
publisher	MDPI AG
series	Foods
issn	2304-8158
publishDate	2021-05-01
description	Multiplex PCR methods have been frequently used for authentication of meat product adulteration. Through screening of new species-specific primers designed based on the mitochondrial DNA sequences, a septuple PCR method is ultimately developed and optimized to simultaneously detect seven species including turkey (110 bp), goose (194 bp), pig (254 bp), sheep (329 bp), beef (473 bp), chicken (612 bp) and duck (718 bp) in one reaction. The proposed method has been validated to be specific, sensitive, robust and inexpensive. Taken together, the developed septuple PCR assay is reliable and efficient, not only to authenticate animal species in commercial meat products, but also easily feasible in a general laboratory without special infrastructures.
topic	septuple PCR adulteration meat species mitochondrial genes multiplex PCR
url	https://www.mdpi.com/2304-8158/10/5/1083
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A Simple and Reliable Single Tube Septuple PCR Assay for Simultaneous Identification of Seven Meat Species

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