Respiratory syncytial virus B sequence analysis reveals a novel early genotype
Abstract Respiratory syncytial virus (RSV) is a major cause of respiratory infections and is classified in two main groups, RSV-A and RSV-B, with multiple genotypes within each of them. For RSV-B, more than 30 genotypes have been described, without consensus on their definition. The lack of genotype...
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2021-02-01
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Online Access: | https://doi.org/10.1038/s41598-021-83079-2 |
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doaj-6bd4dbf975dd46d39d2a4da56eac95b62021-02-14T12:31:31ZengNature Publishing GroupScientific Reports2045-23222021-02-0111111110.1038/s41598-021-83079-2Respiratory syncytial virus B sequence analysis reveals a novel early genotypeJuan C. Muñoz-Escalante0Andreu Comas-García1Sofía Bernal-Silva2Daniel E. Noyola3Microbiology Department, Facultad de Medicina, Universidad Autónoma de San Luis PotosíMicrobiology Department, Facultad de Medicina, Universidad Autónoma de San Luis PotosíMicrobiology Department, Facultad de Medicina, Universidad Autónoma de San Luis PotosíMicrobiology Department, Facultad de Medicina, Universidad Autónoma de San Luis PotosíAbstract Respiratory syncytial virus (RSV) is a major cause of respiratory infections and is classified in two main groups, RSV-A and RSV-B, with multiple genotypes within each of them. For RSV-B, more than 30 genotypes have been described, without consensus on their definition. The lack of genotype assignation criteria has a direct impact on viral evolution understanding, development of viral detection methods as well as vaccines design. Here we analyzed the totality of complete RSV-B G gene ectodomain sequences published in GenBank until September 2018 (n = 2190) including 478 complete genome sequences using maximum likelihood and Bayesian phylogenetic analyses, as well as intergenotypic and intragenotypic distance matrices, in order to generate a systematic genotype assignation. Individual RSV-B genes were also assessed using maximum likelihood phylogenetic analyses and multiple sequence alignments were used to identify molecular markers associated to specific genotypes. Analyses of the complete G gene ectodomain region, sequences clustering patterns, and the presence of molecular markers of each individual gene indicate that the 37 previously described genotypes can be classified into fifteen distinct genotypes: BA, BA-C, BA-CC, CB1-THB, GB1-GB4, GB6, JAB1-NZB2, SAB1, SAB2, SAB4, URU2 and a novel early circulating genotype characterized in the present study and designated GB0.https://doi.org/10.1038/s41598-021-83079-2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juan C. Muñoz-Escalante Andreu Comas-García Sofía Bernal-Silva Daniel E. Noyola |
spellingShingle |
Juan C. Muñoz-Escalante Andreu Comas-García Sofía Bernal-Silva Daniel E. Noyola Respiratory syncytial virus B sequence analysis reveals a novel early genotype Scientific Reports |
author_facet |
Juan C. Muñoz-Escalante Andreu Comas-García Sofía Bernal-Silva Daniel E. Noyola |
author_sort |
Juan C. Muñoz-Escalante |
title |
Respiratory syncytial virus B sequence analysis reveals a novel early genotype |
title_short |
Respiratory syncytial virus B sequence analysis reveals a novel early genotype |
title_full |
Respiratory syncytial virus B sequence analysis reveals a novel early genotype |
title_fullStr |
Respiratory syncytial virus B sequence analysis reveals a novel early genotype |
title_full_unstemmed |
Respiratory syncytial virus B sequence analysis reveals a novel early genotype |
title_sort |
respiratory syncytial virus b sequence analysis reveals a novel early genotype |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-02-01 |
description |
Abstract Respiratory syncytial virus (RSV) is a major cause of respiratory infections and is classified in two main groups, RSV-A and RSV-B, with multiple genotypes within each of them. For RSV-B, more than 30 genotypes have been described, without consensus on their definition. The lack of genotype assignation criteria has a direct impact on viral evolution understanding, development of viral detection methods as well as vaccines design. Here we analyzed the totality of complete RSV-B G gene ectodomain sequences published in GenBank until September 2018 (n = 2190) including 478 complete genome sequences using maximum likelihood and Bayesian phylogenetic analyses, as well as intergenotypic and intragenotypic distance matrices, in order to generate a systematic genotype assignation. Individual RSV-B genes were also assessed using maximum likelihood phylogenetic analyses and multiple sequence alignments were used to identify molecular markers associated to specific genotypes. Analyses of the complete G gene ectodomain region, sequences clustering patterns, and the presence of molecular markers of each individual gene indicate that the 37 previously described genotypes can be classified into fifteen distinct genotypes: BA, BA-C, BA-CC, CB1-THB, GB1-GB4, GB6, JAB1-NZB2, SAB1, SAB2, SAB4, URU2 and a novel early circulating genotype characterized in the present study and designated GB0. |
url |
https://doi.org/10.1038/s41598-021-83079-2 |
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