Integrative Analysis of Methylation and Transcriptional Profiles to Reveal the Genetic Stability of Cashmere Traits in the Tβ4 Overexpression of Cashmere Goats
DNA methylation alteration is frequently observed in exogenous gene silencing and may play important roles in the genetic stability of traits. Cashmere is derived from the secondary hair follicles (SHFs) of cashmere goats, which are morphogenetically distinct from primary hair follicles (PHFs). Here...
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doaj-6be49e9147d24ec7af43cdb75cc6eb682020-11-25T01:58:53ZengMDPI AGAnimals2076-26152019-11-01912100210.3390/ani9121002ani9121002Integrative Analysis of Methylation and Transcriptional Profiles to Reveal the Genetic Stability of Cashmere Traits in the Tβ4 Overexpression of Cashmere GoatsBai Dai0Meng Zhang1Jian-Long Yuan2Li-Qing Ren3Xiao-Yu Han4Dong-Jun Liu5State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaDNA methylation alteration is frequently observed in exogenous gene silencing and may play important roles in the genetic stability of traits. Cashmere is derived from the secondary hair follicles (SHFs) of cashmere goats, which are morphogenetically distinct from primary hair follicles (PHFs). Here, in light of having initially produced 15 T<i>β</i>4 overexpression (T<i>β</i>4-OE) cashmere goats which had more SHFs than the wild type (WT) goats, and produced more cashmere, we produced T<i>β</i>4-OE offsprings both via somatic cell nuclear transfer (SCNT) and via natural mating (NM). However, the desired trait exhibited lower fixation in the line-bred offspring compared to the SCNT offspring. Integrative analysis of methylation and transcriptional profiles showed that this might be due to the influence of methylation on the expression of differentially expressed genes (DEGs) between generations, which was mutually consistent with the results of the functional and pathway enrichment analysis of differentially methylated regions (DMRs) and DEGs. Overall, our study systematically describes the DNA methylation characteristics between generations of cashmere goats and provides a basis for improving genetic stability.https://www.mdpi.com/2076-2615/9/12/1002tβ4genetic stabilitymethylationtranscriptomesomatic cell nuclear transfer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bai Dai Meng Zhang Jian-Long Yuan Li-Qing Ren Xiao-Yu Han Dong-Jun Liu |
spellingShingle |
Bai Dai Meng Zhang Jian-Long Yuan Li-Qing Ren Xiao-Yu Han Dong-Jun Liu Integrative Analysis of Methylation and Transcriptional Profiles to Reveal the Genetic Stability of Cashmere Traits in the Tβ4 Overexpression of Cashmere Goats Animals tβ4 genetic stability methylation transcriptome somatic cell nuclear transfer |
author_facet |
Bai Dai Meng Zhang Jian-Long Yuan Li-Qing Ren Xiao-Yu Han Dong-Jun Liu |
author_sort |
Bai Dai |
title |
Integrative Analysis of Methylation and Transcriptional Profiles to Reveal the Genetic Stability of Cashmere Traits in the Tβ4 Overexpression of Cashmere Goats |
title_short |
Integrative Analysis of Methylation and Transcriptional Profiles to Reveal the Genetic Stability of Cashmere Traits in the Tβ4 Overexpression of Cashmere Goats |
title_full |
Integrative Analysis of Methylation and Transcriptional Profiles to Reveal the Genetic Stability of Cashmere Traits in the Tβ4 Overexpression of Cashmere Goats |
title_fullStr |
Integrative Analysis of Methylation and Transcriptional Profiles to Reveal the Genetic Stability of Cashmere Traits in the Tβ4 Overexpression of Cashmere Goats |
title_full_unstemmed |
Integrative Analysis of Methylation and Transcriptional Profiles to Reveal the Genetic Stability of Cashmere Traits in the Tβ4 Overexpression of Cashmere Goats |
title_sort |
integrative analysis of methylation and transcriptional profiles to reveal the genetic stability of cashmere traits in the tβ4 overexpression of cashmere goats |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2019-11-01 |
description |
DNA methylation alteration is frequently observed in exogenous gene silencing and may play important roles in the genetic stability of traits. Cashmere is derived from the secondary hair follicles (SHFs) of cashmere goats, which are morphogenetically distinct from primary hair follicles (PHFs). Here, in light of having initially produced 15 T<i>β</i>4 overexpression (T<i>β</i>4-OE) cashmere goats which had more SHFs than the wild type (WT) goats, and produced more cashmere, we produced T<i>β</i>4-OE offsprings both via somatic cell nuclear transfer (SCNT) and via natural mating (NM). However, the desired trait exhibited lower fixation in the line-bred offspring compared to the SCNT offspring. Integrative analysis of methylation and transcriptional profiles showed that this might be due to the influence of methylation on the expression of differentially expressed genes (DEGs) between generations, which was mutually consistent with the results of the functional and pathway enrichment analysis of differentially methylated regions (DMRs) and DEGs. Overall, our study systematically describes the DNA methylation characteristics between generations of cashmere goats and provides a basis for improving genetic stability. |
topic |
tβ4 genetic stability methylation transcriptome somatic cell nuclear transfer |
url |
https://www.mdpi.com/2076-2615/9/12/1002 |
work_keys_str_mv |
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