Escitalopram Targets Oxidative Stress, Caspase-3, BDNF and MeCP2 in the Hippocampus and Frontal Cortex of a Rat Model of Depression Induced by Chronic Unpredictable Mild Stress
In recent years, escitalopram (ESC) has been suggested to have different mechanisms of action beyond its well known selective serotonin reuptake inhibition. The aim of this study is to investigate the effects of escitalopram on oxidative stress, apoptosis, brain-derived neurotrophic factor (BDNF), M...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-07-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/22/14/7483 |
id |
doaj-6be91e4393784025ad550c6934d7bfcd |
---|---|
record_format |
Article |
spelling |
doaj-6be91e4393784025ad550c6934d7bfcd2021-07-23T13:46:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-01227483748310.3390/ijms22147483Escitalopram Targets Oxidative Stress, Caspase-3, BDNF and MeCP2 in the Hippocampus and Frontal Cortex of a Rat Model of Depression Induced by Chronic Unpredictable Mild StressVlad Dionisie0Adela Magdalena Ciobanu1Vlad Alexandru Toma2Mihnea Costin Manea3Ioana Baldea4Diana Olteanu5Alexandra Sevastre-Berghian6Simona Clichici7Mirela Manea8Sorin Riga9Gabriela Adriana Filip10Department of Psychiatry and Psychology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartment of Psychiatry, ‘Prof. Dr. Alexandru Obregia’ Clinical Hospital of Psychiatry, 041914 Bucharest, RomaniaDepartment of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babes-Bolyai University, 400028 Cluj-Napoca, RomaniaDepartment of Psychiatry and Psychology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartment of Physiology, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400006 Cluj-Napoca, RomaniaDepartment of Physiology, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400006 Cluj-Napoca, RomaniaDepartment of Physiology, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400006 Cluj-Napoca, RomaniaDepartment of Physiology, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400006 Cluj-Napoca, RomaniaDepartment of Psychiatry and Psychology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartment of Stress Research and Prophylaxis, ‘Prof. Dr. Alexandru Obregia’ Clinical Hospital of Psychiatry, 041914 Bucharest, RomaniaDepartment of Physiology, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400006 Cluj-Napoca, RomaniaIn recent years, escitalopram (ESC) has been suggested to have different mechanisms of action beyond its well known selective serotonin reuptake inhibition. The aim of this study is to investigate the effects of escitalopram on oxidative stress, apoptosis, brain-derived neurotrophic factor (BDNF), Methyl-CpG-binding protein 2 (MeCP2), and oligodendrocytes number in the brain of chronic unpredictable mild stress-induced depressed rats. The animals were randomised in four groups (8 in each group): control, stress, stress + ESC 5 and stress + ESC 5/10. ESC was administered for 42 days in a fixed dose (5 mg/kg b.w.) or in an up-titration regimen (21 days ESC 5 mg/kg b.w. then 21 days ESC 10 mg/kg b.w.). Sucrose preference test (SPT) and elevated plus maze (EPM) were also performed. ESC improved the percentage of sucrose preference, locomotion and anxiety. ESC5/10 reduced the oxidative damage in the hippocampus and improved the antioxidant defence in the hippocampus and frontal lobe. ESC5/10 lowered caspase 3 activity in the hippocampus. Escitalopram had a modulatory effect on BDNF and the number of oligodendrocytes in the hippocampus and frontal lobe and also improved the MeCP2 expressions. The results confirm the multiple pathways implicated in the pathogenesis of depression and suggest that escitalopram exerts an antidepressant effect via different intricate mechanisms.https://www.mdpi.com/1422-0067/22/14/7483escitalopramantidepressantchronic unpredictable mild stressdepressioncaspaseoxidative stress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vlad Dionisie Adela Magdalena Ciobanu Vlad Alexandru Toma Mihnea Costin Manea Ioana Baldea Diana Olteanu Alexandra Sevastre-Berghian Simona Clichici Mirela Manea Sorin Riga Gabriela Adriana Filip |
spellingShingle |
Vlad Dionisie Adela Magdalena Ciobanu Vlad Alexandru Toma Mihnea Costin Manea Ioana Baldea Diana Olteanu Alexandra Sevastre-Berghian Simona Clichici Mirela Manea Sorin Riga Gabriela Adriana Filip Escitalopram Targets Oxidative Stress, Caspase-3, BDNF and MeCP2 in the Hippocampus and Frontal Cortex of a Rat Model of Depression Induced by Chronic Unpredictable Mild Stress International Journal of Molecular Sciences escitalopram antidepressant chronic unpredictable mild stress depression caspase oxidative stress |
author_facet |
Vlad Dionisie Adela Magdalena Ciobanu Vlad Alexandru Toma Mihnea Costin Manea Ioana Baldea Diana Olteanu Alexandra Sevastre-Berghian Simona Clichici Mirela Manea Sorin Riga Gabriela Adriana Filip |
author_sort |
Vlad Dionisie |
title |
Escitalopram Targets Oxidative Stress, Caspase-3, BDNF and MeCP2 in the Hippocampus and Frontal Cortex of a Rat Model of Depression Induced by Chronic Unpredictable Mild Stress |
title_short |
Escitalopram Targets Oxidative Stress, Caspase-3, BDNF and MeCP2 in the Hippocampus and Frontal Cortex of a Rat Model of Depression Induced by Chronic Unpredictable Mild Stress |
title_full |
Escitalopram Targets Oxidative Stress, Caspase-3, BDNF and MeCP2 in the Hippocampus and Frontal Cortex of a Rat Model of Depression Induced by Chronic Unpredictable Mild Stress |
title_fullStr |
Escitalopram Targets Oxidative Stress, Caspase-3, BDNF and MeCP2 in the Hippocampus and Frontal Cortex of a Rat Model of Depression Induced by Chronic Unpredictable Mild Stress |
title_full_unstemmed |
Escitalopram Targets Oxidative Stress, Caspase-3, BDNF and MeCP2 in the Hippocampus and Frontal Cortex of a Rat Model of Depression Induced by Chronic Unpredictable Mild Stress |
title_sort |
escitalopram targets oxidative stress, caspase-3, bdnf and mecp2 in the hippocampus and frontal cortex of a rat model of depression induced by chronic unpredictable mild stress |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-07-01 |
description |
In recent years, escitalopram (ESC) has been suggested to have different mechanisms of action beyond its well known selective serotonin reuptake inhibition. The aim of this study is to investigate the effects of escitalopram on oxidative stress, apoptosis, brain-derived neurotrophic factor (BDNF), Methyl-CpG-binding protein 2 (MeCP2), and oligodendrocytes number in the brain of chronic unpredictable mild stress-induced depressed rats. The animals were randomised in four groups (8 in each group): control, stress, stress + ESC 5 and stress + ESC 5/10. ESC was administered for 42 days in a fixed dose (5 mg/kg b.w.) or in an up-titration regimen (21 days ESC 5 mg/kg b.w. then 21 days ESC 10 mg/kg b.w.). Sucrose preference test (SPT) and elevated plus maze (EPM) were also performed. ESC improved the percentage of sucrose preference, locomotion and anxiety. ESC5/10 reduced the oxidative damage in the hippocampus and improved the antioxidant defence in the hippocampus and frontal lobe. ESC5/10 lowered caspase 3 activity in the hippocampus. Escitalopram had a modulatory effect on BDNF and the number of oligodendrocytes in the hippocampus and frontal lobe and also improved the MeCP2 expressions. The results confirm the multiple pathways implicated in the pathogenesis of depression and suggest that escitalopram exerts an antidepressant effect via different intricate mechanisms. |
topic |
escitalopram antidepressant chronic unpredictable mild stress depression caspase oxidative stress |
url |
https://www.mdpi.com/1422-0067/22/14/7483 |
work_keys_str_mv |
AT vladdionisie escitalopramtargetsoxidativestresscaspase3bdnfandmecp2inthehippocampusandfrontalcortexofaratmodelofdepressioninducedbychronicunpredictablemildstress AT adelamagdalenaciobanu escitalopramtargetsoxidativestresscaspase3bdnfandmecp2inthehippocampusandfrontalcortexofaratmodelofdepressioninducedbychronicunpredictablemildstress AT vladalexandrutoma escitalopramtargetsoxidativestresscaspase3bdnfandmecp2inthehippocampusandfrontalcortexofaratmodelofdepressioninducedbychronicunpredictablemildstress AT mihneacostinmanea escitalopramtargetsoxidativestresscaspase3bdnfandmecp2inthehippocampusandfrontalcortexofaratmodelofdepressioninducedbychronicunpredictablemildstress AT ioanabaldea escitalopramtargetsoxidativestresscaspase3bdnfandmecp2inthehippocampusandfrontalcortexofaratmodelofdepressioninducedbychronicunpredictablemildstress AT dianaolteanu escitalopramtargetsoxidativestresscaspase3bdnfandmecp2inthehippocampusandfrontalcortexofaratmodelofdepressioninducedbychronicunpredictablemildstress AT alexandrasevastreberghian escitalopramtargetsoxidativestresscaspase3bdnfandmecp2inthehippocampusandfrontalcortexofaratmodelofdepressioninducedbychronicunpredictablemildstress AT simonaclichici escitalopramtargetsoxidativestresscaspase3bdnfandmecp2inthehippocampusandfrontalcortexofaratmodelofdepressioninducedbychronicunpredictablemildstress AT mirelamanea escitalopramtargetsoxidativestresscaspase3bdnfandmecp2inthehippocampusandfrontalcortexofaratmodelofdepressioninducedbychronicunpredictablemildstress AT sorinriga escitalopramtargetsoxidativestresscaspase3bdnfandmecp2inthehippocampusandfrontalcortexofaratmodelofdepressioninducedbychronicunpredictablemildstress AT gabrielaadrianafilip escitalopramtargetsoxidativestresscaspase3bdnfandmecp2inthehippocampusandfrontalcortexofaratmodelofdepressioninducedbychronicunpredictablemildstress |
_version_ |
1721287910217482240 |