Diagnostic Assay for Rickettsia japonica

Diagnostic Assay for Rickettsia japonica

We developed a specific and rapid detection system for Rickettsia japonica and R. heilongjiangensis, the causative agents of spotted fever, using a TaqMan minor groove binder probe for a particular open reading frame (ORF) identified by the R. japonica genome project. The target ORF was present only...

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Bibliographic Details
Main Authors: Nozomu Hanaoka, Minenosuke Matsutani, Hiroki Kawabata, Seigo Yamamoto, Hiromi Fujita, Akiko Sakata, Yoshinao Azuma, Motohiko Ogawa, Ai Takano, Haruo Watanabe, Toshio Kishimoto, Mutsunori Shirai, Ichiro Kurane, Ichiro Takajo
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2009-12-01
Series:Emerging Infectious Diseases
Subjects:
Rickettsia japonica
specific ORF
real-time PCR
diagnosis
genome
bacteria
Online Access:https://wwwnc.cdc.gov/eid/article/15/12/09-0252_article
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spelling doaj-6bea9af511234b0b9cd6f66e42e9dc262020-11-24T23:35:30ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592009-12-0115121994199710.3201/eid1512.090252Diagnostic Assay for Rickettsia japonicaNozomu HanaokaMinenosuke MatsutaniHiroki KawabataSeigo YamamotoHiromi FujitaAkiko SakataYoshinao AzumaMotohiko OgawaAi TakanoHaruo WatanabeToshio KishimotoMutsunori ShiraiIchiro KuraneIchiro TakajoWe developed a specific and rapid detection system for Rickettsia japonica and R. heilongjiangensis, the causative agents of spotted fever, using a TaqMan minor groove binder probe for a particular open reading frame (ORF) identified by the R. japonica genome project. The target ORF was present only in R. japonica–related strains.https://wwwnc.cdc.gov/eid/article/15/12/09-0252_articleRickettsia japonicaspecific ORFreal-time PCRdiagnosisgenomebacteria
collection DOAJ
language English
format Article
sources DOAJ
author Nozomu Hanaoka
Minenosuke Matsutani
Hiroki Kawabata
Seigo Yamamoto
Hiromi Fujita
Akiko Sakata
Yoshinao Azuma
Motohiko Ogawa
Ai Takano
Haruo Watanabe
Toshio Kishimoto
Mutsunori Shirai
Ichiro Kurane
Ichiro Takajo
spellingShingle Nozomu Hanaoka
Minenosuke Matsutani
Hiroki Kawabata
Seigo Yamamoto
Hiromi Fujita
Akiko Sakata
Yoshinao Azuma
Motohiko Ogawa
Ai Takano
Haruo Watanabe
Toshio Kishimoto
Mutsunori Shirai
Ichiro Kurane
Ichiro Takajo
Diagnostic Assay for Rickettsia japonica
Emerging Infectious Diseases
Rickettsia japonica
specific ORF
real-time PCR
diagnosis
genome
bacteria
author_facet Nozomu Hanaoka
Minenosuke Matsutani
Hiroki Kawabata
Seigo Yamamoto
Hiromi Fujita
Akiko Sakata
Yoshinao Azuma
Motohiko Ogawa
Ai Takano
Haruo Watanabe
Toshio Kishimoto
Mutsunori Shirai
Ichiro Kurane
Ichiro Takajo
author_sort Nozomu Hanaoka
title Diagnostic Assay for Rickettsia japonica
title_short Diagnostic Assay for Rickettsia japonica
title_full Diagnostic Assay for Rickettsia japonica
title_fullStr Diagnostic Assay for Rickettsia japonica
title_full_unstemmed Diagnostic Assay for Rickettsia japonica
title_sort diagnostic assay for rickettsia japonica
publisher Centers for Disease Control and Prevention
series Emerging Infectious Diseases
issn 1080-6040
1080-6059
publishDate 2009-12-01
description We developed a specific and rapid detection system for Rickettsia japonica and R. heilongjiangensis, the causative agents of spotted fever, using a TaqMan minor groove binder probe for a particular open reading frame (ORF) identified by the R. japonica genome project. The target ORF was present only in R. japonica–related strains.
topic Rickettsia japonica
specific ORF
real-time PCR
diagnosis
genome
bacteria
url https://wwwnc.cdc.gov/eid/article/15/12/09-0252_article
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