THE PECULIARITIES OF CLONAL MICROPROPAGATION OF CANNA GARDEN (Canna × Hybrida hort.)

• Main Authors: Tevfik A. Sh., Mitrofanova I. V., Kuzmina T. N.
• Format: Article
• Language: English
• Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2014-10-01
• Series: Biotechnologia Acta
• Subjects: canna; regeneration; meristemoid
• Online Access: http://biotechnology.kiev.ua/images/storage/5_2014/tevfic.pdf

Regenerants of Canna (Canna × hybrida hort.) cvs. Suevia, Dar Vostoka and Livadia have been obtained. Dependence of viability and regenerative capacity of Canna microshoots on genotype has been found. The optimal concentrations of growth regulators that influence on morphogenesis in vitro have been determined. At the micropropagation stage of different cultivars of Canna it needs the addition of 1.24–1.91 mg/l thidiazuron to culture medium. The active rooting of Canna microshoots cvs. Dar Vostoka and Suevia on the culture medium Murashige & Skoog and Pierik with auxins β-indol-3- acetic acid and α-naphthylacetic acid have been observed. The ability of Canna cv. Suevia microshoots to spontaneous root formation has been shown. The formation of meristemoids in culture in vitro of Canna explants have been confirmed by the results of histological investigations.