Differences of aroma development and metabolic pathway gene expression between Kyoho and 87-1 grapes
Aroma is an important quality trait of grapes and often the focus of consumers, viticulturists and grapevine breeders. Kyoho is a hybrid between Vitis vinifera and Vitis labrusca with a strawberry-like scent, while 87-1 is an early-ripening mutant of Muscat hamburg, belonging to Vitis vinifera, with...
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doaj-6c2b96932eab47099a9c7e7fe61d79362021-06-08T04:43:21ZengElsevierJournal of Integrative Agriculture2095-31192021-06-0120615251539Differences of aroma development and metabolic pathway gene expression between Kyoho and 87-1 grapesXiao-hao JI0Bao-liang WANG1Xiao-di WANG2Xiao-long WANG3Feng-zhi LIU4Hai-bo WANG5JI Xiao-hao, Mobile: +86-13610890936; Research Institute of Pomology, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Germplasm Resources Utilization), Ministry of Agriculture/Key Laboratory of Mineral Nutrition and Efficient Fertilization for Deciduous Fruits, Liaoning Province, Xingcheng 125100, P.R.ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Germplasm Resources Utilization), Ministry of Agriculture/Key Laboratory of Mineral Nutrition and Efficient Fertilization for Deciduous Fruits, Liaoning Province, Xingcheng 125100, P.R.ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Germplasm Resources Utilization), Ministry of Agriculture/Key Laboratory of Mineral Nutrition and Efficient Fertilization for Deciduous Fruits, Liaoning Province, Xingcheng 125100, P.R.ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Germplasm Resources Utilization), Ministry of Agriculture/Key Laboratory of Mineral Nutrition and Efficient Fertilization for Deciduous Fruits, Liaoning Province, Xingcheng 125100, P.R.ChinaCorrespondence LIU Feng-zhi; Research Institute of Pomology, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Germplasm Resources Utilization), Ministry of Agriculture/Key Laboratory of Mineral Nutrition and Efficient Fertilization for Deciduous Fruits, Liaoning Province, Xingcheng 125100, P.R.ChinaCorrespondence WANG Hai-bo; Research Institute of Pomology, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Germplasm Resources Utilization), Ministry of Agriculture/Key Laboratory of Mineral Nutrition and Efficient Fertilization for Deciduous Fruits, Liaoning Province, Xingcheng 125100, P.R.ChinaAroma is an important quality trait of grapes and often the focus of consumers, viticulturists and grapevine breeders. Kyoho is a hybrid between Vitis vinifera and Vitis labrusca with a strawberry-like scent, while 87-1 is an early-ripening mutant of Muscat hamburg, belonging to Vitis vinifera, with a rose scent. In this study, we compared their aroma compositions and concentrations during berry development by headspace-SPME combined with gas chromatography-mass spectrometry (GC-MS), and analyzed the expression differences of enzyme-encoding genes in the LOX-HPL, MEP and MVA metabolic pathways by qRT-PCR. Twelve esters were detected in Kyoho during the whole berry development and they were abundant after veraison, but no esters were detected in 87-1 berries. Linalool was the dominant terpene among the 14 terpenes detected in 87-1 berries, while limited amounts of terpenes were detected in Kyoho berries. qRT-PCR analysis indicated that the low expression of VvAAT might explain the low content of ester volatiles in 87-1 berries, and the low expression of coding genes in the MEP pathway, especially VvPNLinNer1, might be the reason for the low content of volatile terpenes in Kyoho berries. The results from this work will promote our understanding of aroma metabolic mechanisms of grapes, and offer some suggestions for grape aromatic quality improvement.http://www.sciencedirect.com/science/article/pii/S2095311920634815grapeKyoho87-1aromaLOX-HPLMEP |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiao-hao JI Bao-liang WANG Xiao-di WANG Xiao-long WANG Feng-zhi LIU Hai-bo WANG |
spellingShingle |
Xiao-hao JI Bao-liang WANG Xiao-di WANG Xiao-long WANG Feng-zhi LIU Hai-bo WANG Differences of aroma development and metabolic pathway gene expression between Kyoho and 87-1 grapes Journal of Integrative Agriculture grape Kyoho 87-1 aroma LOX-HPL MEP |
author_facet |
Xiao-hao JI Bao-liang WANG Xiao-di WANG Xiao-long WANG Feng-zhi LIU Hai-bo WANG |
author_sort |
Xiao-hao JI |
title |
Differences of aroma development and metabolic pathway gene expression between Kyoho and 87-1 grapes |
title_short |
Differences of aroma development and metabolic pathway gene expression between Kyoho and 87-1 grapes |
title_full |
Differences of aroma development and metabolic pathway gene expression between Kyoho and 87-1 grapes |
title_fullStr |
Differences of aroma development and metabolic pathway gene expression between Kyoho and 87-1 grapes |
title_full_unstemmed |
Differences of aroma development and metabolic pathway gene expression between Kyoho and 87-1 grapes |
title_sort |
differences of aroma development and metabolic pathway gene expression between kyoho and 87-1 grapes |
publisher |
Elsevier |
series |
Journal of Integrative Agriculture |
issn |
2095-3119 |
publishDate |
2021-06-01 |
description |
Aroma is an important quality trait of grapes and often the focus of consumers, viticulturists and grapevine breeders. Kyoho is a hybrid between Vitis vinifera and Vitis labrusca with a strawberry-like scent, while 87-1 is an early-ripening mutant of Muscat hamburg, belonging to Vitis vinifera, with a rose scent. In this study, we compared their aroma compositions and concentrations during berry development by headspace-SPME combined with gas chromatography-mass spectrometry (GC-MS), and analyzed the expression differences of enzyme-encoding genes in the LOX-HPL, MEP and MVA metabolic pathways by qRT-PCR. Twelve esters were detected in Kyoho during the whole berry development and they were abundant after veraison, but no esters were detected in 87-1 berries. Linalool was the dominant terpene among the 14 terpenes detected in 87-1 berries, while limited amounts of terpenes were detected in Kyoho berries. qRT-PCR analysis indicated that the low expression of VvAAT might explain the low content of ester volatiles in 87-1 berries, and the low expression of coding genes in the MEP pathway, especially VvPNLinNer1, might be the reason for the low content of volatile terpenes in Kyoho berries. The results from this work will promote our understanding of aroma metabolic mechanisms of grapes, and offer some suggestions for grape aromatic quality improvement. |
topic |
grape Kyoho 87-1 aroma LOX-HPL MEP |
url |
http://www.sciencedirect.com/science/article/pii/S2095311920634815 |
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