The effect of low concentrations of ethanol on gastric adenocarcinoma cell lines

• Main Authors: Wu Lingjiao, Chen Shaohua, Zhang Yu, Pan Hongming
• Format: Article
• Language: English
• Published: University of Belgrade, University of Novi Sad 2013-01-01
• Series: Archives of Biological Sciences
• Subjects: ethanol; gastric adenocarcinoma; apoptosis; alcohol dehydrogenase
• Online Access: http://www.doiserbia.nb.rs/img/doi/0354-4664/2013/0354-46641302493W.pdf
• ISSN: 0354-4664; 1821-4339

Chronic alcohol consumption was identified as a significant risk factor for cancer in humans. The aim of the study was to analyze the influence of low concentrations of ethanol on gastric adenocarcinoma cell viability, apoptosis, and changes in the expression of alcohol dehydrogenase with ethanol treatment. Gastric adenocarcinoma cell lines (MGC803, MGC823 and SGC7901) were treated with different concentrations of ethanol (0.03125%, 0.0625%, 0.125%, 0.25%, 0.5%, 1%, 2%, and 4%). The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry were used to analyze the effect of ethanol treatment on cell viability and apoptosis. Western blotting was used to analyze the expression of alcohol dehydrogenase in gastric carcinoma cells. Ethanol treatment inhibited cell proliferation in gastric adenocarcinoma cell lines in a significant dose-dependent manner. Ethanol induced apoptosis of gastric adenocarcinoma cells in a dose-dependent manner. The alcohol dehydrogenase activity of gastric adenocarcinoma cells increased with the increase in the concentration of ethanol. Ethanol inhibited cell viability and the growth of gastric adenocarcinoma cell lines. Low concentrations of ethanol also induced apoptosis and increased the expression of alcohol dehydrogenase of the gastric adenocarcinoma cell lines.