Modulation of TLR 3, 7 and 8 Expressions in HCV Genotype 3 Infected Individuals: Potential Correlations of Pathogenesis and Spontaneous Clearance
Background. Hepatitis C virus is the major cause of chronic hepatitis worldwide which finally leads to the development of hepatocellular carcinoma. Toll like receptors (TLRs) play an important role in the course of many viral infections, but the role of TLRs in HCV pathogenesis has not been well elu...
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doaj-6c4d459dc5ce431aa6ec5096c4c916f02020-11-24T21:06:31ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/491064491064Modulation of TLR 3, 7 and 8 Expressions in HCV Genotype 3 Infected Individuals: Potential Correlations of Pathogenesis and Spontaneous ClearanceRushna Firdaus0Aritra Biswas1Kallol Saha2Anirban Mukherjee3Falguni Pal4Sujit Chaudhuri5Alok Chandra6Asokananda Konar7Provash Chandra Sadhukhan8I.C.M.R. Virus Unit Kolkata, ID and BG Hospital Campus, GB-4 (East Wing), 1st Floor, 57 Dr. Suresh Chandra Banerjee Road, Beliaghata, Kolkata, West Bengal 700010, IndiaI.C.M.R. Virus Unit Kolkata, ID and BG Hospital Campus, GB-4 (East Wing), 1st Floor, 57 Dr. Suresh Chandra Banerjee Road, Beliaghata, Kolkata, West Bengal 700010, IndiaI.C.M.R. Virus Unit Kolkata, ID and BG Hospital Campus, GB-4 (East Wing), 1st Floor, 57 Dr. Suresh Chandra Banerjee Road, Beliaghata, Kolkata, West Bengal 700010, IndiaI.C.M.R. Virus Unit Kolkata, ID and BG Hospital Campus, GB-4 (East Wing), 1st Floor, 57 Dr. Suresh Chandra Banerjee Road, Beliaghata, Kolkata, West Bengal 700010, IndiaI.C.M.R. Virus Unit Kolkata, ID and BG Hospital Campus, GB-4 (East Wing), 1st Floor, 57 Dr. Suresh Chandra Banerjee Road, Beliaghata, Kolkata, West Bengal 700010, IndiaDepartment of Gastroenterology, AMRI Hospitals Salt Lake, JC - 16 & 17, Salt Lake City, Kolkata 700091, IndiaDepartment of Medicine and Gastroenterology, Eastern Command Hospital, Alipore road, Kolkata 700027, IndiaDepartment of Gastroenterology, Peerless Hospital and B.K. Roy Research Centre, Kolkata 700094, IndiaI.C.M.R. Virus Unit Kolkata, ID and BG Hospital Campus, GB-4 (East Wing), 1st Floor, 57 Dr. Suresh Chandra Banerjee Road, Beliaghata, Kolkata, West Bengal 700010, IndiaBackground. Hepatitis C virus is the major cause of chronic hepatitis worldwide which finally leads to the development of hepatocellular carcinoma. Toll like receptors (TLRs) play an important role in the course of many viral infections, but the role of TLRs in HCV pathogenesis has not been well elucidated so far. Objective. The aim of this study was to analyse the mRNA expression of TLRs 3, 7, and 8 in different stages of HCV infection including chronic, cirrhosis, interferon treated resolved, and relapsed cases. Methodology. Total RNA from whole blood was extracted and mRNA expression of TLRs 3, 7, and 8 genes was analyzed by quantitative real-time RT-PCR using β-Actin gene as an internal control. Results. This study consisted of 100 HCV infected individuals and twenty healthy controls. TLR 3 expression was found to be significantly elevated in individuals who had spontaneously cleared the virus (p<0.001), whereas TLR 7 was found to be 3.26 times more elevated in patients with cirrhosis of liver. In IFN induced individuals, TLR 8 expression levels were found to be 2.28-fold elevated as compared to control population. Conclusion. TLRs 3, 7, and 8 are prime biomarker candidates for HCV infection mRNA expression analysis which might improve current therapeutic approaches.http://dx.doi.org/10.1155/2014/491064 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rushna Firdaus Aritra Biswas Kallol Saha Anirban Mukherjee Falguni Pal Sujit Chaudhuri Alok Chandra Asokananda Konar Provash Chandra Sadhukhan |
spellingShingle |
Rushna Firdaus Aritra Biswas Kallol Saha Anirban Mukherjee Falguni Pal Sujit Chaudhuri Alok Chandra Asokananda Konar Provash Chandra Sadhukhan Modulation of TLR 3, 7 and 8 Expressions in HCV Genotype 3 Infected Individuals: Potential Correlations of Pathogenesis and Spontaneous Clearance BioMed Research International |
author_facet |
Rushna Firdaus Aritra Biswas Kallol Saha Anirban Mukherjee Falguni Pal Sujit Chaudhuri Alok Chandra Asokananda Konar Provash Chandra Sadhukhan |
author_sort |
Rushna Firdaus |
title |
Modulation of TLR 3, 7 and 8 Expressions in HCV Genotype 3 Infected Individuals: Potential Correlations of Pathogenesis and Spontaneous Clearance |
title_short |
Modulation of TLR 3, 7 and 8 Expressions in HCV Genotype 3 Infected Individuals: Potential Correlations of Pathogenesis and Spontaneous Clearance |
title_full |
Modulation of TLR 3, 7 and 8 Expressions in HCV Genotype 3 Infected Individuals: Potential Correlations of Pathogenesis and Spontaneous Clearance |
title_fullStr |
Modulation of TLR 3, 7 and 8 Expressions in HCV Genotype 3 Infected Individuals: Potential Correlations of Pathogenesis and Spontaneous Clearance |
title_full_unstemmed |
Modulation of TLR 3, 7 and 8 Expressions in HCV Genotype 3 Infected Individuals: Potential Correlations of Pathogenesis and Spontaneous Clearance |
title_sort |
modulation of tlr 3, 7 and 8 expressions in hcv genotype 3 infected individuals: potential correlations of pathogenesis and spontaneous clearance |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2014-01-01 |
description |
Background. Hepatitis C virus is the major cause of chronic hepatitis worldwide which finally leads to the development of hepatocellular carcinoma. Toll like receptors (TLRs) play an important role in the course of many viral infections, but the role of TLRs in HCV pathogenesis has not been well elucidated so far. Objective. The aim of this study was to analyse the mRNA expression of TLRs 3, 7, and 8 in different stages of HCV infection including chronic, cirrhosis, interferon treated resolved, and relapsed cases. Methodology. Total RNA from whole blood was extracted and mRNA expression of TLRs 3, 7, and 8 genes was analyzed by quantitative real-time RT-PCR using β-Actin gene as an internal control. Results. This study consisted of 100 HCV infected individuals and twenty healthy controls. TLR 3 expression was found to be significantly elevated in individuals who had spontaneously cleared the virus (p<0.001), whereas TLR 7 was found to be 3.26 times more elevated in patients with cirrhosis of liver. In IFN induced individuals, TLR 8 expression levels were found to be 2.28-fold elevated as compared to control population. Conclusion. TLRs 3, 7, and 8 are prime biomarker candidates for HCV infection mRNA expression analysis which might improve current therapeutic approaches. |
url |
http://dx.doi.org/10.1155/2014/491064 |
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