Light Mediated Generation of Silver Nanoparticles by Spinach Thylakoids/Chloroplasts.
The unique potential of chloroplasts/thylakoids to harness light energy to transport electrons from H2O to various entities was exploited for reduction of Ag+ to generate nanoparticles (NPs). Spinach thylakoids/chloroplasts turned AgNO3 solutions brown in light, but not in dark. Besides showing Ag-N...
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doaj-6c6cc2a7e11147198e746ae8f45c15032020-11-24T22:11:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011112e016793710.1371/journal.pone.0167937Light Mediated Generation of Silver Nanoparticles by Spinach Thylakoids/Chloroplasts.Nisha ShabnamP SharmilaHyunook KimP Pardha-SaradhiThe unique potential of chloroplasts/thylakoids to harness light energy to transport electrons from H2O to various entities was exploited for reduction of Ag+ to generate nanoparticles (NPs). Spinach thylakoids/chloroplasts turned AgNO3 solutions brown in light, but not in dark. Besides showing Ag-NPs specific surface plasmon resonance band, these brown solutions showed presence of 5-30 nm crystalline NPs composed of Ag. Powder X-ray diffraction (PXRD) analysis revealed that Ag-NPs were biphasic composed of face-centered cubic Ag0 and cubic Ag2O. X-ray photoelectron spectroscopy (XPS) data further corroborated the presence of Ag2O in Ag-NPs. Limited formation of Ag-NPs in dark and increased generation of Ag0/Ag2O-NPs with increase in light intensity (photon flux density) by thylakoids/chloroplasts, established the role of light-harvesting photosynthetic machinery in generation of Ag0/Ag2O-NPs. Potential of thylakoids/chloroplasts to generate Ag-NPs from Ag+ on exposure to red and blue wavelength regions of visible light of electromagnetic spectrum, further confirmed the involvement of photosynthetic electron transport in reduction of Ag+ and generation of Ag-NPs. While light energy mediated photosynthetic electron transport donates energized electrons extracted from H2O to Ag+ to form Ag0-NPs, O2 released as a by-product during photolysis of H2O oxidizes Ag0 to form Ag2O-NPs. Our findings furnish a novel, simple, economic and green method that can be exploited for commercial production of Ag0/Ag2O-NPs.http://europepmc.org/articles/PMC5148069?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nisha Shabnam P Sharmila Hyunook Kim P Pardha-Saradhi |
spellingShingle |
Nisha Shabnam P Sharmila Hyunook Kim P Pardha-Saradhi Light Mediated Generation of Silver Nanoparticles by Spinach Thylakoids/Chloroplasts. PLoS ONE |
author_facet |
Nisha Shabnam P Sharmila Hyunook Kim P Pardha-Saradhi |
author_sort |
Nisha Shabnam |
title |
Light Mediated Generation of Silver Nanoparticles by Spinach Thylakoids/Chloroplasts. |
title_short |
Light Mediated Generation of Silver Nanoparticles by Spinach Thylakoids/Chloroplasts. |
title_full |
Light Mediated Generation of Silver Nanoparticles by Spinach Thylakoids/Chloroplasts. |
title_fullStr |
Light Mediated Generation of Silver Nanoparticles by Spinach Thylakoids/Chloroplasts. |
title_full_unstemmed |
Light Mediated Generation of Silver Nanoparticles by Spinach Thylakoids/Chloroplasts. |
title_sort |
light mediated generation of silver nanoparticles by spinach thylakoids/chloroplasts. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
The unique potential of chloroplasts/thylakoids to harness light energy to transport electrons from H2O to various entities was exploited for reduction of Ag+ to generate nanoparticles (NPs). Spinach thylakoids/chloroplasts turned AgNO3 solutions brown in light, but not in dark. Besides showing Ag-NPs specific surface plasmon resonance band, these brown solutions showed presence of 5-30 nm crystalline NPs composed of Ag. Powder X-ray diffraction (PXRD) analysis revealed that Ag-NPs were biphasic composed of face-centered cubic Ag0 and cubic Ag2O. X-ray photoelectron spectroscopy (XPS) data further corroborated the presence of Ag2O in Ag-NPs. Limited formation of Ag-NPs in dark and increased generation of Ag0/Ag2O-NPs with increase in light intensity (photon flux density) by thylakoids/chloroplasts, established the role of light-harvesting photosynthetic machinery in generation of Ag0/Ag2O-NPs. Potential of thylakoids/chloroplasts to generate Ag-NPs from Ag+ on exposure to red and blue wavelength regions of visible light of electromagnetic spectrum, further confirmed the involvement of photosynthetic electron transport in reduction of Ag+ and generation of Ag-NPs. While light energy mediated photosynthetic electron transport donates energized electrons extracted from H2O to Ag+ to form Ag0-NPs, O2 released as a by-product during photolysis of H2O oxidizes Ag0 to form Ag2O-NPs. Our findings furnish a novel, simple, economic and green method that can be exploited for commercial production of Ag0/Ag2O-NPs. |
url |
http://europepmc.org/articles/PMC5148069?pdf=render |
work_keys_str_mv |
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