Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital food
Abstract Background Pathogenic biotypes of the Methicillin-resistant Staphylococcus aureus (MRSA) strains are considered to be one of the major cause of food-borne diseases in hospitals. The present investigation was done to study the pattern of antibiotic resistance and prevalence of antibiotic res...
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doaj-6c789dea108545d1921fe45896dc00882020-11-24T23:51:18ZengBMCAntimicrobial Resistance and Infection Control2047-29942017-10-016111110.1186/s13756-017-0257-1Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital foodFarhad Safarpoor Dehkordi0Hasan Gandomi1Afshin Akhondzadeh Basti2Ali Misaghi3Ebrahim Rahimi4Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of TehranDepartment of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of TehranDepartment of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of TehranDepartment of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of TehranDepartment of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad UniversityAbstract Background Pathogenic biotypes of the Methicillin-resistant Staphylococcus aureus (MRSA) strains are considered to be one of the major cause of food-borne diseases in hospitals. The present investigation was done to study the pattern of antibiotic resistance and prevalence of antibiotic resistance genes of different biotypes of the MRSA strains isolated from various types of hospital food samples. Methods Four-hundred and eighty-five raw and cooked hospital food samples were cultured and MRSA strains were identified using the oxacillin and cefoxitin disk diffusion tests and mecA-based PCR amplification. Isolated strains were subjected to biotyping and their antibiotic resistance patterns were analyzed using the disk diffusion and PCR methods. Results Prevalence of S. aureus and MRSA were 9.69 and 7.62%, respectively. Meat and chicken barbecues had the highest prevalence of MRSA. Prevalence of bovine, ovine, poultry and human-based biotypes in the MRSA strains were 8.10, 8.10, 32.43 and 48.64%, respectively. All of the MRSA strains recovered from soup, salad and rice samples were related to human-based biotypes. MRSA strains harbored the highest prevalence of resistance against penicillin (100%), ceftaroline (100%), tetracycline (100%), erythromycin (89.18%) and trimethoprim-sulfamethoxazole (83.78%). TetK (72.97%), ermA (72.97%), msrA (64.86%) and aacA-D (62.16%) were the most commonly detected antibiotic resistance genes. Conclusions Pattern of antibiotic resistance and also distribution of antibiotic resistance genes were related to the biotype of MRSA strains. Presence of multi-drug resistance and also simultaneous presence of several antibiotic resistance genes in some MRSA isolates showed an important public health issue Further researches are required to found additional epidemiological aspects of the MRSA strains in hospital food samples.http://link.springer.com/article/10.1186/s13756-017-0257-1Methicillin-resistant Staphylococcus aureusBiotypesAntimicrobial resistanceHospital food |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Farhad Safarpoor Dehkordi Hasan Gandomi Afshin Akhondzadeh Basti Ali Misaghi Ebrahim Rahimi |
spellingShingle |
Farhad Safarpoor Dehkordi Hasan Gandomi Afshin Akhondzadeh Basti Ali Misaghi Ebrahim Rahimi Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital food Antimicrobial Resistance and Infection Control Methicillin-resistant Staphylococcus aureus Biotypes Antimicrobial resistance Hospital food |
author_facet |
Farhad Safarpoor Dehkordi Hasan Gandomi Afshin Akhondzadeh Basti Ali Misaghi Ebrahim Rahimi |
author_sort |
Farhad Safarpoor Dehkordi |
title |
Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital food |
title_short |
Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital food |
title_full |
Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital food |
title_fullStr |
Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital food |
title_full_unstemmed |
Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital food |
title_sort |
phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant staphylococcus aureus isolated from hospital food |
publisher |
BMC |
series |
Antimicrobial Resistance and Infection Control |
issn |
2047-2994 |
publishDate |
2017-10-01 |
description |
Abstract Background Pathogenic biotypes of the Methicillin-resistant Staphylococcus aureus (MRSA) strains are considered to be one of the major cause of food-borne diseases in hospitals. The present investigation was done to study the pattern of antibiotic resistance and prevalence of antibiotic resistance genes of different biotypes of the MRSA strains isolated from various types of hospital food samples. Methods Four-hundred and eighty-five raw and cooked hospital food samples were cultured and MRSA strains were identified using the oxacillin and cefoxitin disk diffusion tests and mecA-based PCR amplification. Isolated strains were subjected to biotyping and their antibiotic resistance patterns were analyzed using the disk diffusion and PCR methods. Results Prevalence of S. aureus and MRSA were 9.69 and 7.62%, respectively. Meat and chicken barbecues had the highest prevalence of MRSA. Prevalence of bovine, ovine, poultry and human-based biotypes in the MRSA strains were 8.10, 8.10, 32.43 and 48.64%, respectively. All of the MRSA strains recovered from soup, salad and rice samples were related to human-based biotypes. MRSA strains harbored the highest prevalence of resistance against penicillin (100%), ceftaroline (100%), tetracycline (100%), erythromycin (89.18%) and trimethoprim-sulfamethoxazole (83.78%). TetK (72.97%), ermA (72.97%), msrA (64.86%) and aacA-D (62.16%) were the most commonly detected antibiotic resistance genes. Conclusions Pattern of antibiotic resistance and also distribution of antibiotic resistance genes were related to the biotype of MRSA strains. Presence of multi-drug resistance and also simultaneous presence of several antibiotic resistance genes in some MRSA isolates showed an important public health issue Further researches are required to found additional epidemiological aspects of the MRSA strains in hospital food samples. |
topic |
Methicillin-resistant Staphylococcus aureus Biotypes Antimicrobial resistance Hospital food |
url |
http://link.springer.com/article/10.1186/s13756-017-0257-1 |
work_keys_str_mv |
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