Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899
Rhizobium tropici is a Gram-negative bacterium that induces nodules and fixed atmospheric nitrogen in symbiotic association with Phaseolus vulgaris (common bean) and some other leguminous species. Lectins are proteins that specifically bind to carbohydrates and, consequently, modulate different biol...
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doaj-6c83132be7c048b199383866dc89b4362020-11-24T23:44:18ZengMDPI AGMolecules1420-30492013-05-011855792580310.3390/molecules18055792Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899Mayron Alves de VasconcelosCláudio Oliveira CunhaFrancisco Vassiliepe Sousa ArrudaVictor Alves CarneiroRafaela Mesquita BastosFábio Martins MercanteKyria Santiago do NascimentoBenildo Sousa CavadaRicardo Pires dos SantosEdson Holanda TeixeiraRhizobium tropici is a Gram-negative bacterium that induces nodules and fixed atmospheric nitrogen in symbiotic association with Phaseolus vulgaris (common bean) and some other leguminous species. Lectins are proteins that specifically bind to carbohydrates and, consequently, modulate different biological functions. In this study, the d-glucose/ d-mannose-binding lectins (from seeds of Dioclea megacarpa, D. rostrata and D. violacea) and D-galactose-binding lectins (from seeds of Bauhinia variegata, Erythina velutina and Vatairea macrocarpa) were purified using chromatographic techniques and evaluated for their effect on the growth of R. tropici CIAT899. All lectins were assayed with a satisfactory degree of purity according to SDS-PAGE analysis, and stimulated bacterial growth; in particular, the Dioclea rostrata lectin was the most active among all tested proteins. As confirmed in the present study, both d-galactose- and d-glucose/d-mannose-binding lectins purified from the seeds of leguminous plants may be powerful biotechnological tools to stimulate the growth of R. tropici CIAT99, thus improving symbiotic interaction between rhizobia and common bean and, hence, the production of this field crop.http://www.mdpi.com/1420-3049/18/5/5792rhizobiaPhaseolus vulgarisstimulusinoculant additive |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mayron Alves de Vasconcelos Cláudio Oliveira Cunha Francisco Vassiliepe Sousa Arruda Victor Alves Carneiro Rafaela Mesquita Bastos Fábio Martins Mercante Kyria Santiago do Nascimento Benildo Sousa Cavada Ricardo Pires dos Santos Edson Holanda Teixeira |
spellingShingle |
Mayron Alves de Vasconcelos Cláudio Oliveira Cunha Francisco Vassiliepe Sousa Arruda Victor Alves Carneiro Rafaela Mesquita Bastos Fábio Martins Mercante Kyria Santiago do Nascimento Benildo Sousa Cavada Ricardo Pires dos Santos Edson Holanda Teixeira Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899 Molecules rhizobia Phaseolus vulgaris stimulus inoculant additive |
author_facet |
Mayron Alves de Vasconcelos Cláudio Oliveira Cunha Francisco Vassiliepe Sousa Arruda Victor Alves Carneiro Rafaela Mesquita Bastos Fábio Martins Mercante Kyria Santiago do Nascimento Benildo Sousa Cavada Ricardo Pires dos Santos Edson Holanda Teixeira |
author_sort |
Mayron Alves de Vasconcelos |
title |
Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899 |
title_short |
Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899 |
title_full |
Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899 |
title_fullStr |
Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899 |
title_full_unstemmed |
Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899 |
title_sort |
effect of leguminous lectins on the growth of rhizobium tropici ciat899 |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2013-05-01 |
description |
Rhizobium tropici is a Gram-negative bacterium that induces nodules and fixed atmospheric nitrogen in symbiotic association with Phaseolus vulgaris (common bean) and some other leguminous species. Lectins are proteins that specifically bind to carbohydrates and, consequently, modulate different biological functions. In this study, the d-glucose/ d-mannose-binding lectins (from seeds of Dioclea megacarpa, D. rostrata and D. violacea) and D-galactose-binding lectins (from seeds of Bauhinia variegata, Erythina velutina and Vatairea macrocarpa) were purified using chromatographic techniques and evaluated for their effect on the growth of R. tropici CIAT899. All lectins were assayed with a satisfactory degree of purity according to SDS-PAGE analysis, and stimulated bacterial growth; in particular, the Dioclea rostrata lectin was the most active among all tested proteins. As confirmed in the present study, both d-galactose- and d-glucose/d-mannose-binding lectins purified from the seeds of leguminous plants may be powerful biotechnological tools to stimulate the growth of R. tropici CIAT99, thus improving symbiotic interaction between rhizobia and common bean and, hence, the production of this field crop. |
topic |
rhizobia Phaseolus vulgaris stimulus inoculant additive |
url |
http://www.mdpi.com/1420-3049/18/5/5792 |
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