DNA Self-assembly Catalyzed by Artificial Agents
Abstract Nucleic acids have been shown to be versatile molecules and engineered to produce various nanostructures. However, the poor rate of these uncatalyzed nucleic acid reactions has restricted the development and applications. Herein, we reported a novel finding that DNA self-assembly could be n...
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2017-07-01
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Online Access: | https://doi.org/10.1038/s41598-017-07210-y |
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doaj-6ca7fd9b0a054bc7a9610cd2b750a4392020-12-08T03:09:41ZengNature Publishing GroupScientific Reports2045-23222017-07-01711510.1038/s41598-017-07210-yDNA Self-assembly Catalyzed by Artificial AgentsChao Shi0Yifan Wang1Menghua Zhang2Cuiping Ma3College of Life Sciences, Qingdao UniversityKey Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Marine Science and Biological Engineering, Qingdao University of Science and TechnologyKey Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Marine Science and Biological Engineering, Qingdao University of Science and TechnologyKey Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Marine Science and Biological Engineering, Qingdao University of Science and TechnologyAbstract Nucleic acids have been shown to be versatile molecules and engineered to produce various nanostructures. However, the poor rate of these uncatalyzed nucleic acid reactions has restricted the development and applications. Herein, we reported a novel finding that DNA self-assembly could be nonenzymatically catalyzed by artificial agents with an increasing dissociation rate constant K2. The catalytic role of several artificial agents in DNA self-assembly was verified by real-time fluorescent detection or agarose gel electrophoresis. We found that 20% PEG 200 could significantly catalyze DNA self-assembly and increase the reaction efficiency, such as linear hybridization chain reaction (HCR) and exponential hairpin assembly (EHA). Therefore, we foresee that a fast and efficient DNA self-assembly in structural DNA nanotechnology will be desirable.https://doi.org/10.1038/s41598-017-07210-y |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chao Shi Yifan Wang Menghua Zhang Cuiping Ma |
spellingShingle |
Chao Shi Yifan Wang Menghua Zhang Cuiping Ma DNA Self-assembly Catalyzed by Artificial Agents Scientific Reports |
author_facet |
Chao Shi Yifan Wang Menghua Zhang Cuiping Ma |
author_sort |
Chao Shi |
title |
DNA Self-assembly Catalyzed by Artificial Agents |
title_short |
DNA Self-assembly Catalyzed by Artificial Agents |
title_full |
DNA Self-assembly Catalyzed by Artificial Agents |
title_fullStr |
DNA Self-assembly Catalyzed by Artificial Agents |
title_full_unstemmed |
DNA Self-assembly Catalyzed by Artificial Agents |
title_sort |
dna self-assembly catalyzed by artificial agents |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-07-01 |
description |
Abstract Nucleic acids have been shown to be versatile molecules and engineered to produce various nanostructures. However, the poor rate of these uncatalyzed nucleic acid reactions has restricted the development and applications. Herein, we reported a novel finding that DNA self-assembly could be nonenzymatically catalyzed by artificial agents with an increasing dissociation rate constant K2. The catalytic role of several artificial agents in DNA self-assembly was verified by real-time fluorescent detection or agarose gel electrophoresis. We found that 20% PEG 200 could significantly catalyze DNA self-assembly and increase the reaction efficiency, such as linear hybridization chain reaction (HCR) and exponential hairpin assembly (EHA). Therefore, we foresee that a fast and efficient DNA self-assembly in structural DNA nanotechnology will be desirable. |
url |
https://doi.org/10.1038/s41598-017-07210-y |
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AT chaoshi dnaselfassemblycatalyzedbyartificialagents AT yifanwang dnaselfassemblycatalyzedbyartificialagents AT menghuazhang dnaselfassemblycatalyzedbyartificialagents AT cuipingma dnaselfassemblycatalyzedbyartificialagents |
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