Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria
An efficient chassis for heterologous expression of biosynthetic gene clusters (BGCs) from Gram-negative bacteria is still unavailable. Here, the authors report rational construction of genome-reduced Burkholderials chassis to facilitate production of a class of new compounds by expressing BGC from...
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Nature Publishing Group
2021-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-24645-0 |
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doaj-6cad4f276e794a0a8b1e4afc89aa72cf2021-07-25T11:37:56ZengNature Publishing GroupNature Communications2041-17232021-07-0112111610.1038/s41467-021-24645-0Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteriaJiaqi Liu0Haibo Zhou1Zhiyu Yang2Xue Wang3Hanna Chen4Lin Zhong5Wentao Zheng6Weijing Niu7Sen Wang8Xiangmei Ren9Guannan Zhong10Yan Wang11Xiaoming Ding12Rolf Müller13Youming Zhang14Xiaoying Bian15Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong UniversityHelmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong UniversityHelmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong UniversityHelmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong UniversityHelmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong UniversityHelmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong UniversityHelmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong UniversityHelmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong UniversityCore Facilities for Life and Environmental Sciences, Shandong UniversityCore Facilities for Life and Environmental Sciences, Shandong UniversityHelmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong UniversityCollege of Marine Life Sciences, and Institute of Evolution & Marine Biodiversity, Ocean University of ChinaCollaborative Innovation Center for Genetics and Development, State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences, Fudan UniversityHelmholtz International Lab for Anti-Infectives, Department of Microbial Natural Products, Helmholtz Institute for Pharmaceutical Research, Helmholtz Centre for Infection Research and Department of Pharmacy, Saarland UniversityHelmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong UniversityHelmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong UniversityAn efficient chassis for heterologous expression of biosynthetic gene clusters (BGCs) from Gram-negative bacteria is still unavailable. Here, the authors report rational construction of genome-reduced Burkholderials chassis to facilitate production of a class of new compounds by expressing BGC from Chitinimonas koreensis.https://doi.org/10.1038/s41467-021-24645-0 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Jiaqi Liu Haibo Zhou Zhiyu Yang Xue Wang Hanna Chen Lin Zhong Wentao Zheng Weijing Niu Sen Wang Xiangmei Ren Guannan Zhong Yan Wang Xiaoming Ding Rolf Müller Youming Zhang Xiaoying Bian |
| spellingShingle |
Jiaqi Liu Haibo Zhou Zhiyu Yang Xue Wang Hanna Chen Lin Zhong Wentao Zheng Weijing Niu Sen Wang Xiangmei Ren Guannan Zhong Yan Wang Xiaoming Ding Rolf Müller Youming Zhang Xiaoying Bian Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria Nature Communications |
| author_facet |
Jiaqi Liu Haibo Zhou Zhiyu Yang Xue Wang Hanna Chen Lin Zhong Wentao Zheng Weijing Niu Sen Wang Xiangmei Ren Guannan Zhong Yan Wang Xiaoming Ding Rolf Müller Youming Zhang Xiaoying Bian |
| author_sort |
Jiaqi Liu |
| title |
Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria |
| title_short |
Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria |
| title_full |
Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria |
| title_fullStr |
Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria |
| title_full_unstemmed |
Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria |
| title_sort |
rational construction of genome-reduced burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2021-07-01 |
| description |
An efficient chassis for heterologous expression of biosynthetic gene clusters (BGCs) from Gram-negative bacteria is still unavailable. Here, the authors report rational construction of genome-reduced Burkholderials chassis to facilitate production of a class of new compounds by expressing BGC from Chitinimonas koreensis. |
| url |
https://doi.org/10.1038/s41467-021-24645-0 |
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