Impact of metabolism and growth phase on the hydrogen isotopic composition of microbial fatty acids
Microorganisms are involved in all elemental cycles and therefore it is important to study their metabolism in the natural environment. A recent technique to investigate this is the hydrogen isotopic composition of microbial fatty acids, i.e. heterotrophic microorganisms produce fatty acids enriched...
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doaj-6cb5ca426c664140ab60427f7425c3ae2020-11-24T22:12:42ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-05-01610.3389/fmicb.2015.00408125619Impact of metabolism and growth phase on the hydrogen isotopic composition of microbial fatty acidsSandra Mariam Heinzelmann0Laura eVillanueva1Danielle eSinke-Schoen2Jaap Smede Sinninghe Damsté3Jaap Smede Sinninghe Damsté4Stefan eSchouten5Stefan eSchouten6Marcel Teunis Jan van der Meer7Royal Netherlands Institute for Sea ResearchRoyal Netherlands Institute for Sea ResearchRoyal Netherlands Institute for Sea ResearchRoyal Netherlands Institute for Sea ResearchUtrecht UniversityRoyal Netherlands Institute for Sea ResearchUtrecht UniversityRoyal Netherlands Institute for Sea ResearchMicroorganisms are involved in all elemental cycles and therefore it is important to study their metabolism in the natural environment. A recent technique to investigate this is the hydrogen isotopic composition of microbial fatty acids, i.e. heterotrophic microorganisms produce fatty acids enriched in deuterium (D) while photoautotrophic and chemoautotrophic microorganisms produce fatty acids depleted in D compared to the water in the culture medium (growth water). However, the impact of factors other than metabolism have not been investigated. Here, we evaluate the impact of growth phase compared to metabolism on the hydrogen isotopic composition of fatty acids of different environmentally relevant microorganisms with heterotrophic, photoautotrophic and chemoautotrophic metabolisms. Fatty acids produced by heterotrophs are enriched in D compared to growth water with εlipid/water between 82 ‰ and 359 ‰ when grown on glucose or acetate, respectively. Photoautrophs (εlipid/water between -149 ‰ and -264 ‰) and chemoautotrophs (εlipid/water between -217 ‰ and -275 ‰) produce fatty acids depleted in D. Fatty acids become, in general, enriched by between 4 and 46 ‰ with growth phase which is minor compared to the influence of different metabolisms. Therefore, the D/H ratio of fatty acids is a promising tool to investigate community metabolism in nature.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00408/fullDeuteriumFatty AcidsMetabolismchemoautotrophyheterotrophymicroorganisms |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sandra Mariam Heinzelmann Laura eVillanueva Danielle eSinke-Schoen Jaap Smede Sinninghe Damsté Jaap Smede Sinninghe Damsté Stefan eSchouten Stefan eSchouten Marcel Teunis Jan van der Meer |
spellingShingle |
Sandra Mariam Heinzelmann Laura eVillanueva Danielle eSinke-Schoen Jaap Smede Sinninghe Damsté Jaap Smede Sinninghe Damsté Stefan eSchouten Stefan eSchouten Marcel Teunis Jan van der Meer Impact of metabolism and growth phase on the hydrogen isotopic composition of microbial fatty acids Frontiers in Microbiology Deuterium Fatty Acids Metabolism chemoautotrophy heterotrophy microorganisms |
author_facet |
Sandra Mariam Heinzelmann Laura eVillanueva Danielle eSinke-Schoen Jaap Smede Sinninghe Damsté Jaap Smede Sinninghe Damsté Stefan eSchouten Stefan eSchouten Marcel Teunis Jan van der Meer |
author_sort |
Sandra Mariam Heinzelmann |
title |
Impact of metabolism and growth phase on the hydrogen isotopic composition of microbial fatty acids |
title_short |
Impact of metabolism and growth phase on the hydrogen isotopic composition of microbial fatty acids |
title_full |
Impact of metabolism and growth phase on the hydrogen isotopic composition of microbial fatty acids |
title_fullStr |
Impact of metabolism and growth phase on the hydrogen isotopic composition of microbial fatty acids |
title_full_unstemmed |
Impact of metabolism and growth phase on the hydrogen isotopic composition of microbial fatty acids |
title_sort |
impact of metabolism and growth phase on the hydrogen isotopic composition of microbial fatty acids |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2015-05-01 |
description |
Microorganisms are involved in all elemental cycles and therefore it is important to study their metabolism in the natural environment. A recent technique to investigate this is the hydrogen isotopic composition of microbial fatty acids, i.e. heterotrophic microorganisms produce fatty acids enriched in deuterium (D) while photoautotrophic and chemoautotrophic microorganisms produce fatty acids depleted in D compared to the water in the culture medium (growth water). However, the impact of factors other than metabolism have not been investigated. Here, we evaluate the impact of growth phase compared to metabolism on the hydrogen isotopic composition of fatty acids of different environmentally relevant microorganisms with heterotrophic, photoautotrophic and chemoautotrophic metabolisms. Fatty acids produced by heterotrophs are enriched in D compared to growth water with εlipid/water between 82 ‰ and 359 ‰ when grown on glucose or acetate, respectively. Photoautrophs (εlipid/water between -149 ‰ and -264 ‰) and chemoautotrophs (εlipid/water between -217 ‰ and -275 ‰) produce fatty acids depleted in D. Fatty acids become, in general, enriched by between 4 and 46 ‰ with growth phase which is minor compared to the influence of different metabolisms. Therefore, the D/H ratio of fatty acids is a promising tool to investigate community metabolism in nature. |
topic |
Deuterium Fatty Acids Metabolism chemoautotrophy heterotrophy microorganisms |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00408/full |
work_keys_str_mv |
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