Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma
Semaphorins were discovered as guidance signals that mediate neural development. Recent studies suggest that semaphorin 3A (Sema3A), a member of the semaphorin family, is involved in the development of several cancers. This study aimed to analyze the association of Sema3A with the clinical features...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/3/423 |
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doaj-6ce2d1a4814344d5b2241fafe63bdf97 |
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Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Tomoko Imoto Satoru Kondo Naohiro Wakisaka Pham Tahnh Hai Noriko Seishima Makoto Kano Takayoshi Ueno Harue Mizokami Yosuke Nakanishi Miyako Hatano Kazuhira Endo Hisashi Sugimoto Makiko Moriyama-Kita Tomokazu Yoshizaki |
| spellingShingle |
Tomoko Imoto Satoru Kondo Naohiro Wakisaka Pham Tahnh Hai Noriko Seishima Makoto Kano Takayoshi Ueno Harue Mizokami Yosuke Nakanishi Miyako Hatano Kazuhira Endo Hisashi Sugimoto Makiko Moriyama-Kita Tomokazu Yoshizaki Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma Microorganisms nasopharyngeal carcinoma semaphorin epstein–barr virus latent membrane protein 1 (lmp1) prognosis |
| author_facet |
Tomoko Imoto Satoru Kondo Naohiro Wakisaka Pham Tahnh Hai Noriko Seishima Makoto Kano Takayoshi Ueno Harue Mizokami Yosuke Nakanishi Miyako Hatano Kazuhira Endo Hisashi Sugimoto Makiko Moriyama-Kita Tomokazu Yoshizaki |
| author_sort |
Tomoko Imoto |
| title |
Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma |
| title_short |
Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma |
| title_full |
Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma |
| title_fullStr |
Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma |
| title_full_unstemmed |
Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma |
| title_sort |
overexpression of semaphorin 3a is a marker associated with poor prognosis in patients with nasopharyngeal carcinoma |
| publisher |
MDPI AG |
| series |
Microorganisms |
| issn |
2076-2607 |
| publishDate |
2020-03-01 |
| description |
Semaphorins were discovered as guidance signals that mediate neural development. Recent studies suggest that semaphorin 3A (Sema3A), a member of the semaphorin family, is involved in the development of several cancers. This study aimed to analyze the association of Sema3A with the clinical features of nasopharyngeal carcinoma (NPC), an Epstein−Barr virus-associated carcinoma, and the Epstein−Barr virus primary oncogene latent membrane protein 1 (LMP1). The expression of Sema3A and LMP1 was immunohistochemically examined in the 35 NPC specimens. The mean expression scores for Sema3A and LMP1 were 20.8% ± 14.5% and 13.9% ± 14.8%, respectively. The expression of Sema3A significantly correlated with that of LMP1 (r = 0.41, <i>p</i> = 0.014). In addition, the Sema3A high cohort showed significantly poorer prognosis than the Sema3A low cohort. Sema3A expression was higher in the LMP1-positive KH-1 and KR-4 cell lines compared to the LMP1-negative HeLa cells. Overexpression of LMP1 in the LMP1-negative AdAH cell line upregulated Sema3A expression, both at the transcriptional and translational level. Finally, Sema3A expression was associated with poor prognosis in patients with NPC. Our data suggest that LMP1 induces the expression of Sema3A, which may promote tumor progression in NPC. |
| topic |
nasopharyngeal carcinoma semaphorin epstein–barr virus latent membrane protein 1 (lmp1) prognosis |
| url |
https://www.mdpi.com/2076-2607/8/3/423 |
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doaj-6ce2d1a4814344d5b2241fafe63bdf972020-11-25T02:39:34ZengMDPI AGMicroorganisms2076-26072020-03-018342310.3390/microorganisms8030423microorganisms8030423Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal CarcinomaTomoko Imoto0Satoru Kondo1Naohiro Wakisaka2Pham Tahnh Hai3Noriko Seishima4Makoto Kano5Takayoshi Ueno6Harue Mizokami7Yosuke Nakanishi8Miyako Hatano9Kazuhira Endo10Hisashi Sugimoto11Makiko Moriyama-Kita12Tomokazu Yoshizaki13Division of Otolaryngology and Head and Neck Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara, Kanazawa, Ishikawa 920-8641, JapanDivision of Otolaryngology and Head and Neck Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara, Kanazawa, Ishikawa 920-8641, JapanDivision of Otolaryngology and Head and Neck Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara, Kanazawa, Ishikawa 920-8641, JapanDivision of Otolaryngology and Head and Neck Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara, Kanazawa, Ishikawa 920-8641, JapanDivision of Otolaryngology and Head and Neck Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara, Kanazawa, Ishikawa 920-8641, JapanDivision of Otolaryngology and Head and Neck Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara, Kanazawa, Ishikawa 920-8641, JapanDivision of Otolaryngology and Head and Neck Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara, Kanazawa, Ishikawa 920-8641, JapanDivision of Otolaryngology and Head and Neck Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara, Kanazawa, Ishikawa 920-8641, JapanDivision of Otolaryngology and Head and Neck Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara, Kanazawa, Ishikawa 920-8641, JapanDivision of Otolaryngology and Head and Neck Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara, Kanazawa, Ishikawa 920-8641, JapanDivision of Otolaryngology and Head and Neck Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara, Kanazawa, Ishikawa 920-8641, JapanDivision of Otolaryngology and Head and Neck Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara, Kanazawa, Ishikawa 920-8641, JapanDivision of Otolaryngology and Head and Neck Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara, Kanazawa, Ishikawa 920-8641, JapanDivision of Otolaryngology and Head and Neck Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara, Kanazawa, Ishikawa 920-8641, JapanSemaphorins were discovered as guidance signals that mediate neural development. Recent studies suggest that semaphorin 3A (Sema3A), a member of the semaphorin family, is involved in the development of several cancers. This study aimed to analyze the association of Sema3A with the clinical features of nasopharyngeal carcinoma (NPC), an Epstein−Barr virus-associated carcinoma, and the Epstein−Barr virus primary oncogene latent membrane protein 1 (LMP1). The expression of Sema3A and LMP1 was immunohistochemically examined in the 35 NPC specimens. The mean expression scores for Sema3A and LMP1 were 20.8% ± 14.5% and 13.9% ± 14.8%, respectively. The expression of Sema3A significantly correlated with that of LMP1 (r = 0.41, <i>p</i> = 0.014). In addition, the Sema3A high cohort showed significantly poorer prognosis than the Sema3A low cohort. Sema3A expression was higher in the LMP1-positive KH-1 and KR-4 cell lines compared to the LMP1-negative HeLa cells. Overexpression of LMP1 in the LMP1-negative AdAH cell line upregulated Sema3A expression, both at the transcriptional and translational level. Finally, Sema3A expression was associated with poor prognosis in patients with NPC. Our data suggest that LMP1 induces the expression of Sema3A, which may promote tumor progression in NPC.https://www.mdpi.com/2076-2607/8/3/423nasopharyngeal carcinomasemaphorinepstein–barr viruslatent membrane protein 1 (lmp1)prognosis |