Histological Lesions, Cell Cycle Arrest, Apoptosis and T Cell Subsets Changes of Spleen in Chicken Fed Aflatoxin-contaminated Corn
The purpose of this study was to evaluate the effects of corn naturally contaminated with aflatoxin B1 and aflatoxin B2 on pathological lesions, apoptosis, cell cycle phases and T lymphocyte subsets of spleen, and to provide an experimental basis for understanding the mechanism of aflatoxin-induced...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2014-08-01
|
Series: | International Journal of Environmental Research and Public Health |
Subjects: | |
Online Access: | http://www.mdpi.com/1660-4601/11/8/8567 |
id |
doaj-6d1e96d2312949488470caa825ebd8d4 |
---|---|
record_format |
Article |
spelling |
doaj-6d1e96d2312949488470caa825ebd8d42020-11-25T00:02:49ZengMDPI AGInternational Journal of Environmental Research and Public Health1660-46012014-08-011188567858010.3390/ijerph110808567ijerph110808567Histological Lesions, Cell Cycle Arrest, Apoptosis and T Cell Subsets Changes of Spleen in Chicken Fed Aflatoxin-contaminated CornXi Peng0Keying Zhang1Shiping Bai2Xuemei Ding3Qiufeng Zeng4Jun Yang5Jing Fang6Kejie Chen7College of Veterinary Medicine, Sichuan Agricultural University, Sichuan 625014, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan 625014, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan 625014, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan 625014, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan 625014, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan 625014, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Sichuan 625014, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Sichuan 625014, ChinaThe purpose of this study was to evaluate the effects of corn naturally contaminated with aflatoxin B1 and aflatoxin B2 on pathological lesions, apoptosis, cell cycle phases and T lymphocyte subsets of spleen, and to provide an experimental basis for understanding the mechanism of aflatoxin-induced immunosuppression. A total of 900 COBB500 male broilers were randomly allocated into five groups with six replicates per group and 30 birds per replicate. The experiment lasted for 6 weeks and the five dietary treatments consisted of control, 25% contaminated corn, 50% contaminated corn, 75% contaminated corn and 100% contaminated corn groups. The histopathological spleen lesions from the contaminated corn groups was characterized as congestion of red pulp, increased necrotic cells and vacuoles in the splenic corpuscle and periarterial lymphatic sheath. The contaminated corn intake significantly increased relative weight of spleen, percentages of apoptotic splenocytes, induced cell cycle arrest of splenocytes, increased the percentages of CD3+CD8+ T cells and decreased the ratios of CD3+CD4+ to CD3+CD8+. The results suggest that AFB-induced immunosuppression maybe closely related to the lesions of spleen.http://www.mdpi.com/1660-4601/11/8/8567aflatoxinbroilerpathological lesionapoptosiscell cycle |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xi Peng Keying Zhang Shiping Bai Xuemei Ding Qiufeng Zeng Jun Yang Jing Fang Kejie Chen |
spellingShingle |
Xi Peng Keying Zhang Shiping Bai Xuemei Ding Qiufeng Zeng Jun Yang Jing Fang Kejie Chen Histological Lesions, Cell Cycle Arrest, Apoptosis and T Cell Subsets Changes of Spleen in Chicken Fed Aflatoxin-contaminated Corn International Journal of Environmental Research and Public Health aflatoxin broiler pathological lesion apoptosis cell cycle |
author_facet |
Xi Peng Keying Zhang Shiping Bai Xuemei Ding Qiufeng Zeng Jun Yang Jing Fang Kejie Chen |
author_sort |
Xi Peng |
title |
Histological Lesions, Cell Cycle Arrest, Apoptosis and T Cell Subsets Changes of Spleen in Chicken Fed Aflatoxin-contaminated Corn |
title_short |
Histological Lesions, Cell Cycle Arrest, Apoptosis and T Cell Subsets Changes of Spleen in Chicken Fed Aflatoxin-contaminated Corn |
title_full |
Histological Lesions, Cell Cycle Arrest, Apoptosis and T Cell Subsets Changes of Spleen in Chicken Fed Aflatoxin-contaminated Corn |
title_fullStr |
Histological Lesions, Cell Cycle Arrest, Apoptosis and T Cell Subsets Changes of Spleen in Chicken Fed Aflatoxin-contaminated Corn |
title_full_unstemmed |
Histological Lesions, Cell Cycle Arrest, Apoptosis and T Cell Subsets Changes of Spleen in Chicken Fed Aflatoxin-contaminated Corn |
title_sort |
histological lesions, cell cycle arrest, apoptosis and t cell subsets changes of spleen in chicken fed aflatoxin-contaminated corn |
publisher |
MDPI AG |
series |
International Journal of Environmental Research and Public Health |
issn |
1660-4601 |
publishDate |
2014-08-01 |
description |
The purpose of this study was to evaluate the effects of corn naturally contaminated with aflatoxin B1 and aflatoxin B2 on pathological lesions, apoptosis, cell cycle phases and T lymphocyte subsets of spleen, and to provide an experimental basis for understanding the mechanism of aflatoxin-induced immunosuppression. A total of 900 COBB500 male broilers were randomly allocated into five groups with six replicates per group and 30 birds per replicate. The experiment lasted for 6 weeks and the five dietary treatments consisted of control, 25% contaminated corn, 50% contaminated corn, 75% contaminated corn and 100% contaminated corn groups. The histopathological spleen lesions from the contaminated corn groups was characterized as congestion of red pulp, increased necrotic cells and vacuoles in the splenic corpuscle and periarterial lymphatic sheath. The contaminated corn intake significantly increased relative weight of spleen, percentages of apoptotic splenocytes, induced cell cycle arrest of splenocytes, increased the percentages of CD3+CD8+ T cells and decreased the ratios of CD3+CD4+ to CD3+CD8+. The results suggest that AFB-induced immunosuppression maybe closely related to the lesions of spleen. |
topic |
aflatoxin broiler pathological lesion apoptosis cell cycle |
url |
http://www.mdpi.com/1660-4601/11/8/8567 |
work_keys_str_mv |
AT xipeng histologicallesionscellcyclearrestapoptosisandtcellsubsetschangesofspleeninchickenfedaflatoxincontaminatedcorn AT keyingzhang histologicallesionscellcyclearrestapoptosisandtcellsubsetschangesofspleeninchickenfedaflatoxincontaminatedcorn AT shipingbai histologicallesionscellcyclearrestapoptosisandtcellsubsetschangesofspleeninchickenfedaflatoxincontaminatedcorn AT xuemeiding histologicallesionscellcyclearrestapoptosisandtcellsubsetschangesofspleeninchickenfedaflatoxincontaminatedcorn AT qiufengzeng histologicallesionscellcyclearrestapoptosisandtcellsubsetschangesofspleeninchickenfedaflatoxincontaminatedcorn AT junyang histologicallesionscellcyclearrestapoptosisandtcellsubsetschangesofspleeninchickenfedaflatoxincontaminatedcorn AT jingfang histologicallesionscellcyclearrestapoptosisandtcellsubsetschangesofspleeninchickenfedaflatoxincontaminatedcorn AT kejiechen histologicallesionscellcyclearrestapoptosisandtcellsubsetschangesofspleeninchickenfedaflatoxincontaminatedcorn |
_version_ |
1725436435697238016 |