Evaluation of rat plasma proteins after bothropic venom inoculation and treatment with Mesenquimal stem cells

• Main Authors: Thalita Costa Telles, Ana Flávia Ribeiro Machado Michel, Maria Lúcia, Bruno Martins Melo, Adriane Costa-Val Pimenta Bicalho, Natália Melo Ocarino, Marilia Martins Melo
• Format: Article
• Language: English
• Published: Instituto de Tecnologia do Paraná (Tecpar) 2014-02-01
• Series: Brazilian Archives of Biology and Technology
• Subjects: Rhinocerophis alternatus venom; Rats; Mesenquimal stem cell; Inflammatory proteins
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100005&lng=en&tlng=en

The aim of this work was to evaluate the mesenchymal stem cells treatment of rats with myonecrosis caused by Rhinocerophis alternatus venom through acute phase proteins (APP) profile. The animals were distributed into three experimental groups (G1, G2 and G3). G1 and G2 were inoculated with 120 μg of R. alternatus venom diluted in 200 µL of ultra-pure water in gastrocnemic muscle, while G3 received 200 µL of ultra-pure water. Three days after, G1 was treated with 5 X 10(6) MSC diluted in PBS and G2 and G3 only with PBS. Each three days after the treatments (3rd, 6th, 9th, 12th 15th days), blood of five animals in each group was collected in order to evaluate the APP. A decrease (P<0.05) in α2-globulin fraction was observed in G1 on the 6th day. In G1 and G2, a raise (P<0.05) was observed in β globulin, a common occurrence in the late phases of inflammatory process, although no significant difference was observed between them. Concerning gamma globulins levels, on the 6th day after the treatments, in G1 and G2 groups, increase in the levels was observed. These data showed that the MSC treatment after bothropic envenomation in the rats caused alteration in APP.